线叶金雀花与藏红花水提取物抗疲劳的作用研究
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摘要
目的研究线叶金雀花与藏红花水提物的抗疲劳作用。方法先将32只小鼠随机分为空白对照组、线叶金雀花组、藏红花组,灌胃给药,1次/d,14 d后进行负重游泳实验。然后,将46只小鼠随机分为空白对照组、线叶金雀花+藏红花低剂量组,灌胃给药,1次/d,14 d后进行负重游泳实验并检测血清乳酸含量。根据上述实验结果,将100只小鼠随机分为空白对照组、线叶金雀花+藏红花低剂量组、线叶金雀花+藏红花中剂量组、线叶金雀花+藏红花高剂量组,灌胃给药14 d后,检测肝糖原、肌糖原的含量。结果单独灌胃线叶金雀花、藏红花水提物均不能增加小鼠负重游泳时间,低剂量线叶金雀花与藏红花合用能够延长小鼠负重游泳时间(P<0.05),减少血清乳酸含量(P<0.05)。与空白对照组相比,线叶金雀花+藏红花低、中、高剂量组均能增加小鼠肝脏糖原和肌肉糖原含量(P<0.05或P<0.01)。结论线叶金雀花、藏红花水提物单独给予不具有抗疲劳作用,而二者联合则具有抗疲劳作用。
Objective To study the anti-fatigue effects of aqueous extracts from Xianye Jinquehua(Rooibos,Aspalathus Linears)and Zanghonghua(Saffron,Crocus sativus L.)through classic behavior test(loaded swimming)and detections of blood lactic acid,liver glycogen and muscle glycogen.Methods At first 32 mice were randomly divided into blank control group,Xianye Jinquehua group and Zanghonghua group,and were orally given corresponding medicinals once a day.The test of loaded swimming was conducted in all groups after 14 d.Then 46 mice were randomly divided into blank control group and low-dose XianyeJinquehua+Zanghonghua group,and were orally given corresponding medicinals once a day.The test of loaded swimming and detection of blood lactic acid were conducted in all groups after 14 d.According to above test results,100 mice were randomly divided into blank control group,low-dose Xianye Jinquehua+Zanghonghua group,middose Xianye Jinquehua +Zanghonghua group and high-dose Xianye Jinquehua +Zanghonghua group.After oral medication for 14 d,the content of liver glycogen and muscle glycogen was detected.Results The time of loaded swimming was not improved in Xianye Jinquehuagroup and Zanghonghua group,and it was prolonged(P<0.05)and content of blood lactic acid was decreased(P<0.05)in low-dose Xianye Jinquehua+Zanghonghua group.The content of liver glycogen and muscle glycogen was increased in low-dose Xianye Jinquehua+Zanghonghua group,mid-dose Xianye Jinquehua+Zanghonghua group and high-dose Xianye Jinquehua+Zanghonghua group compared with blank control group(P<0.05 or P<0.01).Conclusion The single administration of single Xianye Jinquehua or single Zanghonghua has no anti-fatigue effect,while combination of them has this effect.
Objective To study the anti-fatigue effects of aqueous extracts from Xianye Jinquehua(Rooibos,Aspalathus Linears)and Zanghonghua(Saffron,Crocus sativus L.)through classic behavior test(loaded swimming)and detections of blood lactic acid,liver glycogen and muscle glycogen.Methods At first 32 mice were randomly divided into blank control group,Xianye Jinquehua group and Zanghonghua group,and were orally given corresponding medicinals once a day.The test of loaded swimming was conducted in all groups after 14 d.Then 46 mice were randomly divided into blank control group and low-dose XianyeJinquehua+Zanghonghua group,and were orally given corresponding medicinals once a day.The test of loaded swimming and detection of blood lactic acid were conducted in all groups after 14 d.According to above test results,100 mice were randomly divided into blank control group,low-dose Xianye Jinquehua+Zanghonghua group,middose Xianye Jinquehua +Zanghonghua group and high-dose Xianye Jinquehua +Zanghonghua group.After oral medication for 14 d,the content of liver glycogen and muscle glycogen was detected.Results The time of loaded swimming was not improved in Xianye Jinquehuagroup and Zanghonghua group,and it was prolonged(P<0.05)and content of blood lactic acid was decreased(P<0.05)in low-dose Xianye Jinquehua+Zanghonghua group.The content of liver glycogen and muscle glycogen was increased in low-dose Xianye Jinquehua+Zanghonghua group,mid-dose Xianye Jinquehua+Zanghonghua group and high-dose Xianye Jinquehua+Zanghonghua group compared with blank control group(P<0.05 or P<0.01).Conclusion The single administration of single Xianye Jinquehua or single Zanghonghua has no anti-fatigue effect,while combination of them has this effect.
引文
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[11]豆瑞,马莉,郭晓冬,等.红景天苷对不同运动状态下小鼠糖、脂肪及蛋白质代谢的影响[J].同济大学学报(医学版),2018,39(1):59-63.Dou R,Ma L,Guo XD,et al.Effects of salidroside on glucose,fat and protein metabolism in mice under different motion status[J].Journal of Tongji University(Medical Science),2018,39(1):59-63.
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[13]戴艳.运动性疲劳的产生机制及恢复措施的研究[J].沈阳体育学院学报,2004,23(3):333-335.Dai Y.Research on mechanism of the sports fatigue and recovery measures[J].Journal of Shenyang Institute of Physical Education,2004,23(3):333-335.
[14]窦丽娜,王秀全,张桂英,等.九味至宝胶囊抗疲劳作用的研究[J].吉林中医药,2008,28(1):57-58.Dou LN,Wang XQ,Zhang GY,et al.Study on antifatigue effect of Jiuwei Zhibao Capsule[J].Jilin Journal of Traditional Chinese Medicine,2008,28(1):57-58.
[15]牛占忠,张喜平,周红军,等.补肾疏肝汤对大鼠抗疲劳的作用机制研究[J].河北中医,2015,37(9):1363-1365.Niu ZZ,Zhang XP,Zhou HJ,et al.Investigation of anti-fatigue effect of Bushen-shugan decoction in rats and its mechanism[J].Hebei J TCM,2015,37(9):1363-1365.
[16]李东哲,马维民.锁阳煎剂对运动大鼠肝脏自由基代谢及肝糖原代谢的影响[J].现代预防医学,2013,40(8):1478-1480.Li DZ,Ma WM.Effects of cynomorium decoction upon the metabolism of free radical and liver glycogen of liver tissue of exercise rats[J].Modern Preventive Medicine,2013,40(8):1478-1480.
[17]In-Sun H,Hwa-Yong L,Hyun-Pyo K,et al.AntiOxidative Effects of Rooibos Tea(Aspalathus linearis)on Immobilization-Induced Oxidative Stress in Rat Brain[J/OL].PLoS ONE,2014,9(1):e87061[2014-01-21].https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897768/.
[18]Iswaldi I,Arráez-Román D,Rodríguez-Medina I,et al.Identification of phenolic compounds in aqueous and ethanolic rooibos extracts(Aspalathus linearis)by HPLC-ESI-MS(TOF/IT)[J].Analytical&Bioanalytical Chemistry,2011,400(10):3643-3654.
[19]Bramati L,Minoggio M,Gardana C,et al.Quantitative characterization of flavonoid compounds in Rooibos tea(Aspalathus linearis)by LC-UV/DAD[J].J Agric Food Chem,2002,50(20):5513-5519.
[2]Kroyer GT.Rooibos Tea:A rich source of antioxidant active polyphenols with health promoting effects,October 13-16,2008[C].Cairo,Egypt,2008.
[3]马青,李亚,唐民科.线叶金雀花对小鼠脑缺血再灌注损伤的保护作用[J].北京中医药大学学报,2017,40(3):193-199.Ma Q,Li Y,Tang MK.Protective effect of Rooibos on cerebral ischemia-reperfusion injury in mice[J].Journal of Beijing University of Traditional Chinese Medicin,2017,40(3):193-199.
[4]Snijman PW,Joubert E,Ferreira D,et al.Antioxidant activity of the dihydrochalcones Aspalathin and Nothofagin and their corresponding flavones in relation to other Rooibos(Aspalathus linearis)Flavonoids,Epigallocatechin Gallate,and Trolox[J].J Agric Food Chem,2009,57(15):6678-6684.
[5]国家药典委员会.中华人民共和国药典:一部[M].2015版.北京:中国医药科技出版社,2015:129.National Pharmacopoeia Committee.Pharmacopoeia of the People's Republic of China:Vol.1[M].2015ed.Beijing:China Medical Science and Technology Press,2015:129.
[6]胡春,谭晓东.某保健品抗疲劳作用的实验研究[J].武汉科技大学学报(自然科学版),2006,29(2):197-198.Hu C,Tan XD.Laboratory study of a health food in antifatigue effect[J].J.of Wuhan Uni of Sci&Tech(Natural Science Edition),2006,29(2):197-198.
[7]史丽萍,马东明,解丽芳,等.力竭性运动对小鼠肝脏超微结构及肝糖原肌糖原含量的影响[J].辽宁中医杂志,2005,32(9):971-972.Shi LP,Ma DM,Xie LF,et al.Influence of exhaustive exercise on liver ultrastructure and content of hepatic glycogen and muscle glycogen in mice[J].Liaoning Journal of Traditional Chinese Medicine,2005,32(9):971-972.
[8]吴涛,杨建雄,李宝茹,等.芦丁抗疲劳作用的实验研究[J].临床医学,2013,33(3):90-92.Wu T,Yang JX,Li BR,et al.Study on anti-fatigue effect of rutin[J].Clinical Medicine,2013,33(3):90-92.
[9]冯玉华,阎润红,段剑飞.升阳益胃汤抗疲劳的实验研究[J].中国实验方剂学杂志,2008,14(8):60-62.Feng YH,Yan RH,Duan JF.The anti-fatigue study of the decoction of Shengyang Yiqi[J].Chinese Journal of Experimental Traditional Medical Formulae,2008,14(8):60-62.
[10]曹晶晶,杨卫杰,曹轶.刺梨多糖的抗氧化和抗疲劳研究[J].中国中医基础医学杂志,2018,24(4):474-476.Cao JJ,Yang WJ,Cao Y.Anti-fatigue and antioxidant activity of the polysaccharides isolated fromRosa Roxburghii Tratt.F.Normalis Rehd.Et Wils[J].Journal of Basic Chinese Medicine,2018,24(4):474-476.
[11]豆瑞,马莉,郭晓冬,等.红景天苷对不同运动状态下小鼠糖、脂肪及蛋白质代谢的影响[J].同济大学学报(医学版),2018,39(1):59-63.Dou R,Ma L,Guo XD,et al.Effects of salidroside on glucose,fat and protein metabolism in mice under different motion status[J].Journal of Tongji University(Medical Science),2018,39(1):59-63.
[12]陈飞,何先元,周卯勤,等.筋骨草对小鼠和大鼠抗疲劳抗氧化的影响[J].中药药理与临床,2015,31(6):100-102.Chen F,He XY,Zhou MQ,et al.Study on the antifatigue and anti-oxidant effects of herbs Schnabelia tetradonta in mice and rats[J].Pharmacology and Clinic of Traditional Chinese Medicine,2015,31(6):100-102.
[13]戴艳.运动性疲劳的产生机制及恢复措施的研究[J].沈阳体育学院学报,2004,23(3):333-335.Dai Y.Research on mechanism of the sports fatigue and recovery measures[J].Journal of Shenyang Institute of Physical Education,2004,23(3):333-335.
[14]窦丽娜,王秀全,张桂英,等.九味至宝胶囊抗疲劳作用的研究[J].吉林中医药,2008,28(1):57-58.Dou LN,Wang XQ,Zhang GY,et al.Study on antifatigue effect of Jiuwei Zhibao Capsule[J].Jilin Journal of Traditional Chinese Medicine,2008,28(1):57-58.
[15]牛占忠,张喜平,周红军,等.补肾疏肝汤对大鼠抗疲劳的作用机制研究[J].河北中医,2015,37(9):1363-1365.Niu ZZ,Zhang XP,Zhou HJ,et al.Investigation of anti-fatigue effect of Bushen-shugan decoction in rats and its mechanism[J].Hebei J TCM,2015,37(9):1363-1365.
[16]李东哲,马维民.锁阳煎剂对运动大鼠肝脏自由基代谢及肝糖原代谢的影响[J].现代预防医学,2013,40(8):1478-1480.Li DZ,Ma WM.Effects of cynomorium decoction upon the metabolism of free radical and liver glycogen of liver tissue of exercise rats[J].Modern Preventive Medicine,2013,40(8):1478-1480.
[17]In-Sun H,Hwa-Yong L,Hyun-Pyo K,et al.AntiOxidative Effects of Rooibos Tea(Aspalathus linearis)on Immobilization-Induced Oxidative Stress in Rat Brain[J/OL].PLoS ONE,2014,9(1):e87061[2014-01-21].https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897768/.
[18]Iswaldi I,Arráez-Román D,Rodríguez-Medina I,et al.Identification of phenolic compounds in aqueous and ethanolic rooibos extracts(Aspalathus linearis)by HPLC-ESI-MS(TOF/IT)[J].Analytical&Bioanalytical Chemistry,2011,400(10):3643-3654.
[19]Bramati L,Minoggio M,Gardana C,et al.Quantitative characterization of flavonoid compounds in Rooibos tea(Aspalathus linearis)by LC-UV/DAD[J].J Agric Food Chem,2002,50(20):5513-5519.