不同采收期乌天麻中多糖的提取及含量测定
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摘要
采用水提醇沉法制备天麻多糖,在单因素试验基础上,考察提取时间、提取温度、液固比对天麻多糖提取工艺的影响,通过Box-Behnken法优化天麻多糖的提取工艺参数,确定最佳提取工艺条件为:液固比34∶1(mL/g)、提取温度77℃、提取时间78 min,在此提取条件下,天麻多糖的得率为31.14%。通过对贵州省大方县不同采收期(立冬、冬至、立春)乌天麻中的多糖含量进行测定,结果显示不同采收期乌天麻中多糖的得率差异显著,其中冬至采收的乌天麻中多糖得率最高,最高可达32.2%,但不同采收期乌天麻中的多糖得率均大于20%,表明在这一段时间内采收均能保证天麻质量。
The polysaccharide of Rhizoma Gastrodiae was prepared by hot water extraction and alcohol precipitation method. The extraction conditions were selected by single factor test and optimized by response surface methodology,and the optimum extraction parameters were as follows:the ratio of solvent to material was 34 ∶ 1(mL/g),the extraction temperature was 77 ℃,and the extraction time was 78 min. Under this condition,the extraction rate of polysaccharides from Rhizoma Gastrodiae was 31.14 %. The optimized extraction conditions were adopted for polysaccharide content determination from Rhizoma Gastrodiae of different harvest periods. There are significant different among the harvest periods. The Gastrodiae Rhizome polysaccharide content is highest in samples harvested in the winter solstice,reaching to 32.2 %. However,the polysaccharide content of Rhizoma Gastrodiae obtained were all greater than 20 %,implying a suitable time for Rhizoma Gastrodiae harvest during this period.
The polysaccharide of Rhizoma Gastrodiae was prepared by hot water extraction and alcohol precipitation method. The extraction conditions were selected by single factor test and optimized by response surface methodology,and the optimum extraction parameters were as follows:the ratio of solvent to material was 34 ∶ 1(mL/g),the extraction temperature was 77 ℃,and the extraction time was 78 min. Under this condition,the extraction rate of polysaccharides from Rhizoma Gastrodiae was 31.14 %. The optimized extraction conditions were adopted for polysaccharide content determination from Rhizoma Gastrodiae of different harvest periods. There are significant different among the harvest periods. The Gastrodiae Rhizome polysaccharide content is highest in samples harvested in the winter solstice,reaching to 32.2 %. However,the polysaccharide content of Rhizoma Gastrodiae obtained were all greater than 20 %,implying a suitable time for Rhizoma Gastrodiae harvest during this period.
引文
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[16]陈哲,冯佳悦,曹诚,等.襄麦冬多糖及皂苷体外活性研究[J].食品研究与开发,2018,39(13):7-12
[17]DUBOIS M,GILLES K A,HAMILTON J K,et al.Colorimetric method for determination of sugars and related substances[J].Analytical chemistry,1956,28(3):350-356
[18]MA F,KONG S,TAN H,et al.Structural characterization and antiviral effect of a novel polysaccharide PSP-2B from Prunellae Spica[J].Carbohydrate Polymers,2016,152(1):699-709
[2]MING J,LIU J,WU S,et al.Structural Characterization and Hypolipidemic Activity of a Polysaccharide PGEB-3H from the Fruiting Bodies of Gastrodia elata Blume[J].Procedia Engineering,2012,37(1):169-173
[3]ZHAN H-D,ZHOU H-Y,SUI Y-P,et al.The rhizome of Gastrodia elata Blume-An ethnopharmacological review[J].Journal of Ethnopharmacology,2016,189(1):361-385
[4]邓向亮.枸杞多糖通过改善肿瘤免疫微环境增强荷瘤小鼠抗肿瘤免疫功能[C].上海:中国化学会·2016全国多糖研讨会,2016:15
[5]陈蕊,高颖,顾宁.黄芪多糖对THP-1源性巨噬细胞TLR4、Syk、Erk及Paxillin蛋白磷酸化水平与细胞吞噬功能的影响[J].中华中医药杂志,2016,31(5):1679-1683
[6]孙雪芳,王洪新,梁灵君,等.黄芪多糖通过TLR4/NF-κB信号通路抑制脂多糖诱导的大鼠心肌细胞肥大[J].中国药理学通报,2013,29(2):208-212
[7]武剑.柴胡多糖对巨噬细胞免疫功能的调节及对TLR4信号通路的影响[D].上海:复旦大学,2012:37-47
[8]邹荣灿,王乾,孙俊,等.国内近10年铁皮石斛多糖研究进展[J].食品研究与开发,2018,39(12):209-214
[9]王晓敏,林霖雨,李康丽,等.超声波辅助提取山药多糖及体外抗氧化性研究[J].食品研究与开发,2018,39(12):24-28
[10]汪瑞敏,朱秋劲,张春花,等.不同提取方法对天麻多糖抗氧化活性的影响[J].食品科技,2015,40(3):208-213
[11]李峰,朱洁平,王艳梅,等.天麻多糖对小鼠免疫性肝损伤的保护作用[J].中药药理与临床,2015,31(1):111-113
[12]刘明学,李琼芳,黄治强,等.天麻多糖提取与自由基清除作用研究[J].食品科技,2008(9):165-168
[13]汪植,容辉,段和平.天麻多糖对小鼠免疫功能的影响[J].中国民族民间医药杂志,2007(2):112-114,124
[14]缪化春,沈业寿.天麻多糖的降血压作用[J].高血压杂志,2006(7):531-534
[15]朱振元,韩丹.滑子菇多糖提取工艺优化[J].食品研究与开发,2018,39(4):68-72
[16]陈哲,冯佳悦,曹诚,等.襄麦冬多糖及皂苷体外活性研究[J].食品研究与开发,2018,39(13):7-12
[17]DUBOIS M,GILLES K A,HAMILTON J K,et al.Colorimetric method for determination of sugars and related substances[J].Analytical chemistry,1956,28(3):350-356
[18]MA F,KONG S,TAN H,et al.Structural characterization and antiviral effect of a novel polysaccharide PSP-2B from Prunellae Spica[J].Carbohydrate Polymers,2016,152(1):699-709