摘要
为揭示水鬼蕉生物碱(AHL)对HepG-2细胞作用,采用FITC-Annexin V/PI双染色法检测AHL对HepG-2细胞凋亡的影响,并检测了AHL对HepG-2细胞内Ca~(2+)浓度、活性氧(ROS)、pH的影响。结果表明:AHL可以通过细胞内ROS、Ca~(2+)、pH三者间相互作用而诱导细胞凋亡。
In order to reveal the effect of alkaloids of Amaryllidaceae plant Hymenocallis littoralis(AHL)on HepG-2 cells,FITC-Annexin V/PI double staining was used to detect the effect of AHL on apoptosis of HepG-2 cells and on Ca~(2+) concentration,reactive oxygen species(ROS)and pH in HepG-2 cells.The results show that:AHL could induce apoptosis through the interaction of ROS,Ca~(2+),and pH in cells.
引文
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