番鸭细小病毒病病原的血清学检测与PCR鉴定
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摘要
为了解安徽省番鸭细小病毒病的流行情况,采用酶联免疫吸附试验(ELISA)对该省内部分番鸭群进行鹅细小病毒的血清抗体检测,同时对疑似病鸭进行了病原的PCR检测。结果发现,在被检测的44份血清样本中鹅细小病毒血清抗体阳性率高达34.1%;PCR检测结果显示,所检测的样品中,有2份为鹅细小病毒阳性,1份为番鸭细小病毒阳性,且3份被检样品均来自雏番鸭。该研究结果表明,该省番鸭群中存在鹅细小病毒和番鸭细小病毒感染,应采取有效的预防控制措施。
To investigate the prevalence of parvovirus disease in Muscovy ducks in Anhui Province, antibodies of the Muscovy ducks serum samples collected from different areas of this province against goose parvovirus was detected by using enzyme-linked immunosorbent assay(ELISA). In addition, PCR assay was used to identify the etiological agents of the Muscovy ducks with clinically suspected parvovirus infection. The results showed that the positive rate of goose parvovirus serum antibody in the44 serum samples tested was as high as 34.1%; PCR results demonstrated that 2 and 1 of the tested samples were positive for the presence of goose parvovirus and Muscovy duck parvovirus, respectively, and all of the 3 positive samples were collected from the Muscovy ducking. Our results identify the prevalence of goose parvovirus and Muscovy duck parvovirus infections in the Muscovy duck population in this region, and effective prevention and control measures should be taken.
To investigate the prevalence of parvovirus disease in Muscovy ducks in Anhui Province, antibodies of the Muscovy ducks serum samples collected from different areas of this province against goose parvovirus was detected by using enzyme-linked immunosorbent assay(ELISA). In addition, PCR assay was used to identify the etiological agents of the Muscovy ducks with clinically suspected parvovirus infection. The results showed that the positive rate of goose parvovirus serum antibody in the44 serum samples tested was as high as 34.1%; PCR results demonstrated that 2 and 1 of the tested samples were positive for the presence of goose parvovirus and Muscovy duck parvovirus, respectively, and all of the 3 positive samples were collected from the Muscovy ducking. Our results identify the prevalence of goose parvovirus and Muscovy duck parvovirus infections in the Muscovy duck population in this region, and effective prevention and control measures should be taken.
引文
[1]吴娟,卢梁萍,戴银,等.番鸭细小病毒病和鹅细小病毒病的特征和防控[J].畜牧与饲料科学,2017,38(8):100-102.
[2]崔国杰,陈少莺.番鸭细小病毒和小鹅瘟病毒混合感染的诊治[J].畜牧与兽医,2015,47(3):147-148.
[3]陈瑶,关淼.雏番鸭细小病毒病的诊断与防制[J].现代畜牧兽医,2010(4):42-44.
[4]程晓霞,陈仕龙,陈少莺,等.番鸭细小病毒和鹅细小病毒的抗原相关性研究[J].福建农业学报,2013,28(9):869-871.
[5]戴银,张丹俊,沈学怀,等.安徽番鸭细小病毒的分离和PCR检测[J].中国兽医杂志,2016,52(8):35-37.
[6]邵红霞,吕亚楠,叶建强,等.2013年苏皖地区鹅细小病毒的分离鉴定及其VP3基因序列分析[J].中国兽医学报,2014,34(12):1916-1921.
[7]黄宇翔,刘力威,李洪彬,等.鹅细小病毒病、副黏病毒病的流行病学调查及病原分离鉴定[J].中国家禽,2013,35(2):22-25.
[8]戴银,张丹俊,赵瑞宏,等.番鸭细小病毒安徽分离株结构基因的克隆及序列分析[J].动物医学进展,2017,38(4):24-27.
[9]张云,耿宏伟,郭东春,等.鹅和番鸭细小病毒全基因克隆与序列分析[J].中国预防兽医学报,2008,30(6):415-419.
[10]刘家森,姜骞,司昌德,等.番鸭细小病毒与鹅细小病毒PCR鉴别诊断方法的建立[J].中国兽医科学,2007,37(6):469-472.
[11]宋永峰,温纳相,宋延华,等.应用PCR快速诊断番鸭细小病毒病和小鹅瘟[J].动物医学进展,2009,30(5):49-52.
[12]鲜思美,文心田,曹三杰,等.鹅细小病毒和番鸭细小病毒双重PCR检测方法的建立[J].畜牧与兽医,2010,42(4):32-35.
[2]崔国杰,陈少莺.番鸭细小病毒和小鹅瘟病毒混合感染的诊治[J].畜牧与兽医,2015,47(3):147-148.
[3]陈瑶,关淼.雏番鸭细小病毒病的诊断与防制[J].现代畜牧兽医,2010(4):42-44.
[4]程晓霞,陈仕龙,陈少莺,等.番鸭细小病毒和鹅细小病毒的抗原相关性研究[J].福建农业学报,2013,28(9):869-871.
[5]戴银,张丹俊,沈学怀,等.安徽番鸭细小病毒的分离和PCR检测[J].中国兽医杂志,2016,52(8):35-37.
[6]邵红霞,吕亚楠,叶建强,等.2013年苏皖地区鹅细小病毒的分离鉴定及其VP3基因序列分析[J].中国兽医学报,2014,34(12):1916-1921.
[7]黄宇翔,刘力威,李洪彬,等.鹅细小病毒病、副黏病毒病的流行病学调查及病原分离鉴定[J].中国家禽,2013,35(2):22-25.
[8]戴银,张丹俊,赵瑞宏,等.番鸭细小病毒安徽分离株结构基因的克隆及序列分析[J].动物医学进展,2017,38(4):24-27.
[9]张云,耿宏伟,郭东春,等.鹅和番鸭细小病毒全基因克隆与序列分析[J].中国预防兽医学报,2008,30(6):415-419.
[10]刘家森,姜骞,司昌德,等.番鸭细小病毒与鹅细小病毒PCR鉴别诊断方法的建立[J].中国兽医科学,2007,37(6):469-472.
[11]宋永峰,温纳相,宋延华,等.应用PCR快速诊断番鸭细小病毒病和小鹅瘟[J].动物医学进展,2009,30(5):49-52.
[12]鲜思美,文心田,曹三杰,等.鹅细小病毒和番鸭细小病毒双重PCR检测方法的建立[J].畜牧与兽医,2010,42(4):32-35.