超滤-纳滤联合用于川贝母生物碱的浓缩工艺
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摘要
目的探索超滤-纳滤联用技术在川贝母生物碱成分浓缩中的应用。方法以总蛋白和西贝母碱透过率为指标,考察超滤过程。在单因素试验的基础上,采用Box-Behnken响应面法,以西贝母碱的截留率为指标,考察滤膜截留相对分子量、超滤液中西贝母碱浓度和p H值对纳滤过程的影响。结果采用截留相对分子量为10 000的滤膜,对总蛋白的去除率为98. 61%,西贝母碱的透过率达到98. 57%。纳滤过程最佳工艺参数:截留相对分子量为500、西贝母碱质量浓度为140μg/ml、超滤液pH为5. 5,对西贝母碱的实际截留率为93. 57%。结论超滤-纳滤联用技术可有效完成对川贝母生物碱成分的浓缩,相比传统热浓缩优势明显。
Objective To explore the application of ultrafiltration-nanofiltration in the concentration of alkaloids fromSichuan Fritillaria. Methods Passing rate of total protein and imperialine were chosen as indicators to investigate the ultrafiltration process. On the basis of single factor test and with cut-off rate of imperialine as indicators,Box-Behnken response surface method was used to investigate the effect of molecular weight cut-off,imperialine concentration of ultrafiltrate solution and pH with indicators such as cut-off rate of imperialine. Results Removal rate of total protein was 98. 61%,and the passing rate of imperialine was 98. 57% by membrane with molecular weight cut-off as 10 000. Optimal nanofiltration process parameters were as follows: molecular weight cut-off was 500,imperialine concentration was 140 μg/ml and p H was 5. 5,and actual cut-off rate of imperialine was 93. 57%. Conclusion Ultrafiltration-nanofiltration coupling technology is more effective in the concentration of alkaloids from Sichuan Fritillaria than traditional thermal concentration.
Objective To explore the application of ultrafiltration-nanofiltration in the concentration of alkaloids fromSichuan Fritillaria. Methods Passing rate of total protein and imperialine were chosen as indicators to investigate the ultrafiltration process. On the basis of single factor test and with cut-off rate of imperialine as indicators,Box-Behnken response surface method was used to investigate the effect of molecular weight cut-off,imperialine concentration of ultrafiltrate solution and pH with indicators such as cut-off rate of imperialine. Results Removal rate of total protein was 98. 61%,and the passing rate of imperialine was 98. 57% by membrane with molecular weight cut-off as 10 000. Optimal nanofiltration process parameters were as follows: molecular weight cut-off was 500,imperialine concentration was 140 μg/ml and p H was 5. 5,and actual cut-off rate of imperialine was 93. 57%. Conclusion Ultrafiltration-nanofiltration coupling technology is more effective in the concentration of alkaloids from Sichuan Fritillaria than traditional thermal concentration.
引文
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[14] Yaroshchuk A,Bruening ML,Bernal EEL. Solution-diffusionelectro-migration model and its uses for analysis of nanofiltration,pressure-retarded osmosis and forw ard osmosis in multi-ionic solutions[J]. J M embr Sci,2013,447(22):463-476.
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[16] Fang J,Deng BL. Rejection and modeling of arsenate by nanofiltration:contributions of convection,diffusion and electromigration to arsenic transport[J]. M embr Sci Technol,2014,453(453):42-51
[2]张荣发.川贝母的研究进展[J].中国药业,2006,15(8):62-64.
[3]李滢,匡雪君,孙超,等.川贝母转录组密码子使用偏好性分析[J].中国中药杂志,2016,41(11):2055-2060.
[4]曹新伟.川贝母的化学成分研究与贝母属药用植物质量评价[D].北京:中国协和医科大学,2008.
[5]曹新伟,张萌,李军,等.川贝母生物碱类成分的研究[J].中草药,2009,40(1):15-17.
[6]刘薇,张文娟,程显隆,等.中药川贝母质量控制方法研究[J].亚太传统医药,2015,11(2):41-46.
[7]杨远姗.渗滤法提取川贝母工艺的研究[J].中国实用医药,2012,7(1):16-17.
[8]张剑光,徐彦,刘圆,等.响应面设计法优化川贝母茎和叶中总生物碱的提取工艺[J].食品科学,2013,34(10):11-15.
[9]王道东,邵珠德,刘元涛.川贝母中西贝母碱提取工艺优选[J].辽宁中医药大学学报,2016,18(5):9-11.
[10]普文英,张卫东,赵汉臣.纳滤--新型的分离小分子有机物的膜技术[J].中国药房,2000,11(5):234.
[11]李存玉,钱祥,杨泽秋,等.基于纳滤技术的消癌平注射液中的钾离子去除工艺优化[J].中成药,2015,37(2):294-297.
[12]林立刚,叶卉,赵莉芝,等.膜吸附和膜吸收研究进展[J].中国工程科学,2014,(12):59-66,75.
[13]陈萍,朱胤龙,韦强,等.均匀设计法优选川贝母渗滤提取工艺[J].中国实验方剂学杂志,2003,9(3):12-13.
[14] Yaroshchuk A,Bruening ML,Bernal EEL. Solution-diffusionelectro-migration model and its uses for analysis of nanofiltration,pressure-retarded osmosis and forw ard osmosis in multi-ionic solutions[J]. J M embr Sci,2013,447(22):463-476.
[15]赵彦彦,袁其朋.有机相纳滤分离过程中浓度、电荷、溶剂对溶质截留行为的影响[J].膜科学与技术,2006,26(5):31-36.
[16] Fang J,Deng BL. Rejection and modeling of arsenate by nanofiltration:contributions of convection,diffusion and electromigration to arsenic transport[J]. M embr Sci Technol,2014,453(453):42-51