骨通贴膏对大鼠急性炎症的抗炎作用研究
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摘要
目的:研究骨通贴膏对大鼠急性炎症的抗炎作用及机制。方法:60只SD大鼠随机分为空白对照组、模型对照组、骨通贴膏低、中、高剂量组(0.594,1.188,2.376 g/贴,以生药计分别为0.48,0.96,1.92 g/贴)、醋酸泼尼松组(0.005 4 g·kg~(-1))。于大鼠右足跖皮下注入5%甲醛,制备大鼠急性炎症模型,测量足跖容积,观察抗炎效果;采用酶联免疫吸附(ELISA)法检测血清和炎症组织中一氧化氮(NO)、5-羟色胺(5-HT)和组胺(HIS)含量,及炎症组织中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)和前列腺素E_2(PGE_2)含量;以HE染色法制备炎症组织病理切片,观察病理变化并进行评分。结果:与模型对照组相比,骨通贴膏可显著抑制致炎大鼠足跖肿胀(P<0.05或P<0.01);同时,对血清和炎症组织中NO、5-HT、HIS的含量有着不同程度的降低作用(P<0.01),并可显著降低炎症组织中PGE_2的含量(P<0.05或P<0.01),升高炎症组织中IL-6的含量,以及改善炎症组织病理变化(病理学评分显著降低)。部分指标与醋酸泼尼松效果相当(P>0.05)。此外,在抑制足跖肿胀,影响炎症介质及改善病理变化方面,骨通贴膏高、中、低剂量组间并未表现出明显的剂量依赖性(P>0.05)。结论:骨通贴膏对甲醛致炎大鼠具有较好的抗炎作用,可能通过降低血清和炎症组织中NO、5-HT、HIS及炎症组织中PGE_2的含量,升高炎症组织中IL-6的含量,改善局部炎症组织的皮下水肿和炎症细胞浸润来发挥作用。
Objective: To study the anti-inflammatory effect and mechanism of Gutong plasters in acute inflammatory model of rats. Methods: Totally 60 SD rats were randomly divided into the blank group,the model group,Gutong plasters at low,medium and high dose groups( 0. 594,1. 188 and 2. 376 g/patch containing crude drug 0. 48,0. 96 and 1. 92 g,respectively) and prednisone acetate group( 0. 005 4 g·kg~(-1)). The acute inflammation model was prepared by injecting 5% formaldehyde into right side of foot plantar. And then,the anti-inflammatory effect was evaluated by measuring the foot plantar volume. Enzyme-linked immunosorbent assay( ELISA) was used to detect the levels of nitric oxide( NO),histamine( HIS) and 5-hydroxytryptamine( 5-HT) in serum and inflammatory tissue,the levels of prostaglandin E_2( PGE_2),tumor necrosis factor alpha( TNF-α),interleukin 1 beta( IL-1β) and interleukin 6( IL-6) in inflammatory tissue were also determined. Pathological changes were observed through the pathological sections prepared by HE staining. Results: When compared with the model group,Gutong plasters could significantly inhibit the swelling of foot plantar in inflammatory rats( P < 0. 01). At the same time,Gutong plasters could significantly reduce the levels of NO,5-HT and HIS in serum and inflammatory tissue in different degrees( P < 0. 05 or P < 0. 01),significantly reduce the content of PGE_2 in inflammatory tissue( P < 0. 05 or P < 0. 01),increase the content of IL-6 in inflammatory tissue and improve the pathological changes of inflammatory tissues( the pathological score was significantly reduced). In partical indictor changes,Gutong plasters and prednisone acetate showed an equal effect. In addition,Gutong plasters didn't show significant dose-dependent manner in inhibiting foot swelling,affecting inflammatory mediators and improving pathological changes( P > 0. 05). Conclusion: Gutong plasters have better anti-inflammatory effect in the acute inflammation model induced by formaldehyde in rats. The anti-inflammatory effect may be associated with the decrease of the levels of NO,5-HT and HIS in serum and inflammatory tissue,the decrease of the content of PGE_2 and the increase of the content of IL-6 in inflammatory tissue. In addition,the anti-inflammatory effect may be relevant to improving the local inflammatory tissue subcutaneous edema and inflammatory cell infiltration.
Objective: To study the anti-inflammatory effect and mechanism of Gutong plasters in acute inflammatory model of rats. Methods: Totally 60 SD rats were randomly divided into the blank group,the model group,Gutong plasters at low,medium and high dose groups( 0. 594,1. 188 and 2. 376 g/patch containing crude drug 0. 48,0. 96 and 1. 92 g,respectively) and prednisone acetate group( 0. 005 4 g·kg~(-1)). The acute inflammation model was prepared by injecting 5% formaldehyde into right side of foot plantar. And then,the anti-inflammatory effect was evaluated by measuring the foot plantar volume. Enzyme-linked immunosorbent assay( ELISA) was used to detect the levels of nitric oxide( NO),histamine( HIS) and 5-hydroxytryptamine( 5-HT) in serum and inflammatory tissue,the levels of prostaglandin E_2( PGE_2),tumor necrosis factor alpha( TNF-α),interleukin 1 beta( IL-1β) and interleukin 6( IL-6) in inflammatory tissue were also determined. Pathological changes were observed through the pathological sections prepared by HE staining. Results: When compared with the model group,Gutong plasters could significantly inhibit the swelling of foot plantar in inflammatory rats( P < 0. 01). At the same time,Gutong plasters could significantly reduce the levels of NO,5-HT and HIS in serum and inflammatory tissue in different degrees( P < 0. 05 or P < 0. 01),significantly reduce the content of PGE_2 in inflammatory tissue( P < 0. 05 or P < 0. 01),increase the content of IL-6 in inflammatory tissue and improve the pathological changes of inflammatory tissues( the pathological score was significantly reduced). In partical indictor changes,Gutong plasters and prednisone acetate showed an equal effect. In addition,Gutong plasters didn't show significant dose-dependent manner in inhibiting foot swelling,affecting inflammatory mediators and improving pathological changes( P > 0. 05). Conclusion: Gutong plasters have better anti-inflammatory effect in the acute inflammation model induced by formaldehyde in rats. The anti-inflammatory effect may be associated with the decrease of the levels of NO,5-HT and HIS in serum and inflammatory tissue,the decrease of the content of PGE_2 and the increase of the content of IL-6 in inflammatory tissue. In addition,the anti-inflammatory effect may be relevant to improving the local inflammatory tissue subcutaneous edema and inflammatory cell infiltration.
引文
1杜婕莹,曾森,王璐,等.聚异丁烯骨通贴膏经皮肤给药的安全性研究[J].中国药房,2016,27(25):3512-3514
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9霍佩琼,马仁强,罗超,等.金不换总生物碱对小鼠的镇痛作用[J].中药新药杂志,2008,17(14):1226-1228
10 吴文君,杜菲菲,刘全旭,等.复方儿茶止泻霜对大鼠足肿胀、小鼠耳肿胀及腹腔毛细血管通透性的影响[J].山东医药,2013,53(25):26-28
11 Hu W,Zhou PH,Rao T,et al.Adrenomedullin attenuates interleukin-1beta-induced inflammation and apoptosis in rat Leydig cells viainhibition of NF-kappa B signaling pathway[J].Exp Cell Res,2015,339(2):220-230
12 Liu W,Chen JL,Liu H,et al.Effects of meglumine cyclic adenylate pretreatment on systemic inflammatory response syndrome induced by lipopolysaccharide in rats[J].J Huazhong Univ Sci Technolog Med Sci,2017,37(3):332-336
13 李艳丽,胡彦武.紫花地丁抗炎作用及机制研究[J].中国实验方剂学杂志,2012,18(4):244-247
14 李靖,宋晓凯.近年来国内外中药抗炎作用机制研究概况[J].实用药物与临床,2012,15(3):171-173
15 林梅英,陈飞虎,葛金芳,等.鬼针草总黄酮对急性炎症的保护作用及可能机制研究[J].中国临床药理学与治疗学,2013,18(6):614-620
2 王冠.骨通贴膏对急性软组织损伤患者疼痛的影响[J].中国医药导刊,2015,17(12):1239-1241
3 徐天博,赵冉.天和骨通贴膏治疗急性腰扭伤50例[J].中国中医药现代化远程教育,2016,14(23):77-78
4 钟立甲,张文泉,华永丽,等.代谢组学方法评价当归不同炮制品挥发油抗大鼠急性炎症作用的研究[J].中国中药杂志,2016,41(11):2061-2069
5 李妤菲,杜婕莹,曾森,等.骨通贴膏对甲醛疼痛模型大鼠的镇痛作用及机制研究[J].中国药房,2017,28(13):1766-1769
6 陈荣明,姜淼,殷书梅,等.复方南星止痛膏对甲醛等致炎性疼痛模型大鼠止痛作用及c-fos表达的影响[J].世界中西医结合杂志,2008,3(8):454-456
7 卞惠敏,俞景华,姜淼.复方南星止痛膏抗炎作用研究[J].中药药理与临床,2007,23(5):164-165
8 杨媛媛,陈志永,廖立平,等.冯了性风湿跌打药酒中东莨菪素、东莨菪苷和绿原酸的测定[J].中成药,2014,36(3):551-553
9霍佩琼,马仁强,罗超,等.金不换总生物碱对小鼠的镇痛作用[J].中药新药杂志,2008,17(14):1226-1228
10 吴文君,杜菲菲,刘全旭,等.复方儿茶止泻霜对大鼠足肿胀、小鼠耳肿胀及腹腔毛细血管通透性的影响[J].山东医药,2013,53(25):26-28
11 Hu W,Zhou PH,Rao T,et al.Adrenomedullin attenuates interleukin-1beta-induced inflammation and apoptosis in rat Leydig cells viainhibition of NF-kappa B signaling pathway[J].Exp Cell Res,2015,339(2):220-230
12 Liu W,Chen JL,Liu H,et al.Effects of meglumine cyclic adenylate pretreatment on systemic inflammatory response syndrome induced by lipopolysaccharide in rats[J].J Huazhong Univ Sci Technolog Med Sci,2017,37(3):332-336
13 李艳丽,胡彦武.紫花地丁抗炎作用及机制研究[J].中国实验方剂学杂志,2012,18(4):244-247
14 李靖,宋晓凯.近年来国内外中药抗炎作用机制研究概况[J].实用药物与临床,2012,15(3):171-173
15 林梅英,陈飞虎,葛金芳,等.鬼针草总黄酮对急性炎症的保护作用及可能机制研究[J].中国临床药理学与治疗学,2013,18(6):614-620