产朊假丝酵母、植物乳杆菌混合发酵及净水效果
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摘要
采用单因素试验和正交试验的方法对产朊假丝酵母Candida utilis和植物乳杆菌Lactobacillus plantarum组成的水产微生态制剂EM12的混合发酵技术及发酵产物的水质净化效果进行了研究。EM12在优化的条件下混合发酵,植物乳杆菌和产朊假丝酵母液体发酵的最高活菌浓度分别达6. 70×109、2. 00×107CFU/m L,固体发酵的最高活菌浓度分别达1. 64×1010、1.65×108CFU/g湿培养基。固体和液体菌剂对模拟水体中45. 0 mg/L亚硝态氮去除率分别为100%和89. 8%。产朊假丝酵母和植物乳杆菌混合同步发酵,提高了有效活菌浓度,保证了水质净化效果,为该复合菌剂在水产养殖上的应用提供了理论基础和技术支撑。
A microbiological agent named EM12 was consisted of Candida utilis and Lactobacillus plantarum.Single factor experiments and orthogonal-design experiments were applied to optimize its liquid-and solid-state mixed fermentation technology,followed by examining the water purification efficiencies of the fermented products. The results showed that under the optimized liquid-state medium and fermentation conditions,the densities of L. plantarum and C. utilis reached up to 6. 70 × 109 CFU/m L and 2. 00 × 107 CFU/m L,respectively. Moreover,with the optimal solid-state medium,the densities of L. plantarum and C. utilis reached up to 1. 64 × 1010 CFU/g and 1. 65 × 108 CFU/g,respectively. The efficiencies of removing 45. 0 mg/L nitrite from simulated sewage by solid and liquid microbial inoculums were 100% and 89. 8%,respectively. Mixed fermentation of L. plantarum and C. utilis not only increased the concentration of effective live bacteria,but also ensured their water purification effects,which provides theoretical basis and support for applying these compounded microbial inoculums in aquaculture.
A microbiological agent named EM12 was consisted of Candida utilis and Lactobacillus plantarum.Single factor experiments and orthogonal-design experiments were applied to optimize its liquid-and solid-state mixed fermentation technology,followed by examining the water purification efficiencies of the fermented products. The results showed that under the optimized liquid-state medium and fermentation conditions,the densities of L. plantarum and C. utilis reached up to 6. 70 × 109 CFU/m L and 2. 00 × 107 CFU/m L,respectively. Moreover,with the optimal solid-state medium,the densities of L. plantarum and C. utilis reached up to 1. 64 × 1010 CFU/g and 1. 65 × 108 CFU/g,respectively. The efficiencies of removing 45. 0 mg/L nitrite from simulated sewage by solid and liquid microbial inoculums were 100% and 89. 8%,respectively. Mixed fermentation of L. plantarum and C. utilis not only increased the concentration of effective live bacteria,but also ensured their water purification effects,which provides theoretical basis and support for applying these compounded microbial inoculums in aquaculture.
引文
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[20]王玉燕,满兆红,都启晶,等.益生菌混合发酵高产工艺优化的研究[J].食品科技,2016,42(6):21-25.
[21]刘乔,姚国强,李晶,等.乳酸菌与酵母菌混合发酵及冻干粉的研究[J].食品工业,2016,37(12):34-38.
[22]刘慧知,高书锋,周映华,等.凝结芽孢杆菌固体发酵条件和培养基优化[J].粮食与饲料工业,2014,12(8):48-53.
[23]蒋欣荣,翟恒贤,陈大卫,等.乳酸菌混合发酵对抑菌作用的影响[J].中国乳品工业,2017,45(9):11-15.
[24]LVAREZ-MURTN P,FLREZ A B, HERNDEZBARRANCO A,et al. Interaction between dairy yeasts and lactic acid bacteria strains during milk fermentation[J]. Food Control,2008,19(1):62-70.
[2]张涛,晨光,张荣飞,等.水产饲料安全及水产微生态制剂的应用进展[J].饲料博览,2013(11):32-35.
[3] RAJOKA M I,AHMED S,HASHMI A S,et a1. Production of microbial biomass protein from mixed substrates by sequential culture fermentation of Candida utilis and Brevibacterium lactofermentum[J]. Annals of Microbiology,2012,62(3):1 173-1 179.
[4]李云莉,高权新,张晨捷,等.养殖水域抗生素抗性基因污染的研究概况与展望[J].海洋渔业,2017,39(3):351-360.
[5]文明,李文辉,郭丹,等.酵母培养物对南美白对虾生长性能、消化酶活性及非特异性免疫的影响[J].中国畜牧杂志,2017,53(4):108-111.
[6] SHARAWY Z,GODA A S,HASSAAN M S. Partial or total replacement of fish meal by solid state fermented soybean meal with Saccharomyces cerevisiae in diets for indian prawn shrimp,Fenneropenaeus Indicus, Posdarvae[J]. Animal Feed Science and Technology,2015,212:90-99.
[7]谢凤行.一种酵母菌和乳酸菌混合同步发酵制备微生态制剂的方法及应用:201710783514.2[P]. 2017-09-04.
[8]王继雯,岳丹丹,赵俊杰,等.两株芽孢杆菌混菌发酵产孢条件的优化[J].中国酿造,2017,36(5):95-99.
[9]胡峻.除臭酵母和乳酸菌共培养方法:201010124153. 9[P]. 2012-06-27.
[10]付宝荣.一种粪链球菌和酵母菌共培养的方法:201310093122. 5[P]. 2013-07-03.
[11]高绪娜,陈玉春,赵倩,等.酵母培养物高活性菌株的分离及发酵条件优化[J].中国饲料,2017(1):13-17;27.
[12]许岱,范光森,富志磊,等.一株高产β-苯乙醇酵母菌的筛选、鉴定及其发酵条件优化[J].食品工业科技,2017,38(5):151-158.
[13]明强强,于丽娜,张伟,等.乳酸菌固态发酵制备花生蛋白肽及抗氧化活性研究[J].花生学报,2013,42(3):8-15.
[14]闫凯,郑维莎,连智雄,等.一株高产酸乳酸菌的分离、鉴定及高密度发酵研究[J].广东饲料,2017,26(6):24-27.
[15]何瑞,廖钰婷,王雪婷,等.复合菌发酵苦荞麸皮馒头的工艺优化[J].食品工业科技,2017,38(5):238-245.
[16]谢凤行.一种饲料发酵剂及在饲料发酵上的应用:201710105964. 6[P]. 2017-02-27.
[17]谢凤行,张峰峰,周可,等.水质净化乳酸菌的分离鉴定及发酵参数优化[J].微生物学报,2017,57(2):304-314.
[18]王欣,赵巍,付丽,等.植物乳杆菌增殖培养基优化[J].饲料工业,2014,359(18):52-57.
[19]白永强,于丽娟,周慧敏,等.植物乳杆菌Zhang-LL高密度发酵的研究[J].中国农学通报,2015,31(31):285-290.
[20]王玉燕,满兆红,都启晶,等.益生菌混合发酵高产工艺优化的研究[J].食品科技,2016,42(6):21-25.
[21]刘乔,姚国强,李晶,等.乳酸菌与酵母菌混合发酵及冻干粉的研究[J].食品工业,2016,37(12):34-38.
[22]刘慧知,高书锋,周映华,等.凝结芽孢杆菌固体发酵条件和培养基优化[J].粮食与饲料工业,2014,12(8):48-53.
[23]蒋欣荣,翟恒贤,陈大卫,等.乳酸菌混合发酵对抑菌作用的影响[J].中国乳品工业,2017,45(9):11-15.
[24]LVAREZ-MURTN P,FLREZ A B, HERNDEZBARRANCO A,et al. Interaction between dairy yeasts and lactic acid bacteria strains during milk fermentation[J]. Food Control,2008,19(1):62-70.