涝渍胁迫下玉米不定根产生候选基因的发掘
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摘要
不定根产生是玉米对涝渍胁迫适应的形态之一,本研究拟发掘调控玉米不定根产生的候选基因。本研究对400份玉米自交系进行涝渍处理,发现自交系T1162W在涝渍胁迫下产生大量的不定根,而自交系RO421W不定根数量很少。以T1162W、RO421W为亲本构建F2分离群体。从F2群体中,选择不定根大量产生和无不定根的单株,分别组成不定根产生池和不定根不产生池,并对2个混合池进行BSR-seq分析。结果发现,在第7染色体39 Mb区段,发现1个与玉米不定根产生关联的SNP。该QTL位点的发现,将为揭示玉米不定根的形成积累了生物学知识,同时为玉米耐渍性遗传改良提供新的基因资源。
The adventitious roots are considered to be one of key adaptation to waterlogging stress in maize. The aim of this study is to identify the novel candidate genes associated with adventitious roots in maize. In this study,400 maize inbred lines were treated with waterlogging. The results showed that a large number of adventitious roots in inbred lines T1162 W were produced under the waterlogging stress, while a small number of adventitious roots in inbred lines RO421 W. T1162 W and RO421 W were chosen to form F2 population as parent. This study selected the single plant with a large number of adventitious roots and a small number of adventitious roots, and divided it into produce pools of adventitious roots and no produce pools of adventitious roots. Then we performed BSR-seq analysis on two mixing pools. The results exhibited that a SNP(near 39 Mb on chromosome 7) associated with the production of adventitious roots. And the explored of QTL locus might accumulate the knowledge of biology to reveal the formation of adventitious roots in corn, as well as provide the new gene resource of genetic improvement for corn waterlogging tolerance.
The adventitious roots are considered to be one of key adaptation to waterlogging stress in maize. The aim of this study is to identify the novel candidate genes associated with adventitious roots in maize. In this study,400 maize inbred lines were treated with waterlogging. The results showed that a large number of adventitious roots in inbred lines T1162 W were produced under the waterlogging stress, while a small number of adventitious roots in inbred lines RO421 W. T1162 W and RO421 W were chosen to form F2 population as parent. This study selected the single plant with a large number of adventitious roots and a small number of adventitious roots, and divided it into produce pools of adventitious roots and no produce pools of adventitious roots. Then we performed BSR-seq analysis on two mixing pools. The results exhibited that a SNP(near 39 Mb on chromosome 7) associated with the production of adventitious roots. And the explored of QTL locus might accumulate the knowledge of biology to reveal the formation of adventitious roots in corn, as well as provide the new gene resource of genetic improvement for corn waterlogging tolerance.
引文
Bacan amwo M.,and Purcell L.C.,1999,Soybean root morphological and anato mical traitsassociated with acclimation to flooding,Crop Sci.,39(1):143-149
Dai J.R.,and E L.Z.,2010,Scientific and technological innova tion of maize breeding in China,Yumi Kexue(Journal of Maize Sciences),18(1):1-5(戴景瑞,鄂立柱,2010,我国玉米育种科技创新问题的几点思考,玉米科学,18(1):1-5)
Du H.W.,Zhu J.X.,Su H.,Huang M.,Wang H.W.,Ding S.C.,Zhang B.L.,Luo A.,Wei S.D.,Tian X.H.,and Xu Y.B.,2017,Bulked segregant RNA-seq reveals differential expression and SNPs of candidate genes associated with waterlogging tolerance in maize,Front.Plant Sci.,8:1022
Mano Y.,and Omorit F.,2009,Breeding for flooding tolerant maize using"teosinte"as a germplasm resource,Plant Root,1:17-21
Mano Y.,Muraki M.,and Takamizoi T.,2006,Identification of QTL controlling flooding tolerance in reducing soil conditions in maize(Zea mays L.)seedlings,Plant Prod Sci.,9(2):176-181
Mano Y.,Muraki M.,Fujimori M.,Takamizo T.,and Kindiger B.,2005a,Identification of QTL controlling adventitious root formation during flooding conditions in teosinte(Zea mays ssp.huehuetenangensis)seedling,Euphytica,142(1):33-42
Mano Y.,Omorit F.,Muraki M.,and Takamizoi T.,2005b,QTLmapping of adventitious root formations under flooding condition in tropical maize(Zea mays L.)seedlings,Breeding Sci.,55:343-347
Mano Y.,Omori F.,and Takeda K.,2012,Construction of intraspecific linkage maps,detection of a chromosome inversion,and mapping of QTL for constitutive root aerenchyma formation in the teosinte Zea nicaraguensis,Mol.Breeding,29(1):137-146
Tase K.,and Kobayashi M.,1996,Evaluation of wintering ability in italian ryegrass(Lolium multittorum Lam)cultivars,J.JPNSoc.Hortic Sci.,42:63-67
Zaidi P.H.,Rashid Z.,Vinayan M.T.,Almeida G.D.,Phagna R.K.,and Babu R.,2015,QTL mapping of agronomic waterlogging tolerance using recombinant lines derived from tropical maize(Zea mays L.)germplasm,PLoS One,10(4):e0124350
Zhao J.R.,and Wang R.H.,2013,Development process,problem and countermeasure of maize production in China,Zhongguo Nongye Keji Daobao(Journal of Agricultural Science and Technology),15(3):1-6(赵久然,王荣焕,2013,中国玉米生产发展历程、存在问题及对策,中国农业科技导报,15(3):1-6)
Zhu J.X.,Su H.,Zeng X.,and Du H.W.,2017,The quality analysis of transcriptome responsive to waterlogging stress in maize,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(2):1-6(朱建雄,苏航,曾秀,杜何为,2017,玉米淹水胁迫转录组质量的分析,分子植物育种,15(2):1-6)
Dai J.R.,and E L.Z.,2010,Scientific and technological innova tion of maize breeding in China,Yumi Kexue(Journal of Maize Sciences),18(1):1-5(戴景瑞,鄂立柱,2010,我国玉米育种科技创新问题的几点思考,玉米科学,18(1):1-5)
Du H.W.,Zhu J.X.,Su H.,Huang M.,Wang H.W.,Ding S.C.,Zhang B.L.,Luo A.,Wei S.D.,Tian X.H.,and Xu Y.B.,2017,Bulked segregant RNA-seq reveals differential expression and SNPs of candidate genes associated with waterlogging tolerance in maize,Front.Plant Sci.,8:1022
Mano Y.,and Omorit F.,2009,Breeding for flooding tolerant maize using"teosinte"as a germplasm resource,Plant Root,1:17-21
Mano Y.,Muraki M.,and Takamizoi T.,2006,Identification of QTL controlling flooding tolerance in reducing soil conditions in maize(Zea mays L.)seedlings,Plant Prod Sci.,9(2):176-181
Mano Y.,Muraki M.,Fujimori M.,Takamizo T.,and Kindiger B.,2005a,Identification of QTL controlling adventitious root formation during flooding conditions in teosinte(Zea mays ssp.huehuetenangensis)seedling,Euphytica,142(1):33-42
Mano Y.,Omorit F.,Muraki M.,and Takamizoi T.,2005b,QTLmapping of adventitious root formations under flooding condition in tropical maize(Zea mays L.)seedlings,Breeding Sci.,55:343-347
Mano Y.,Omori F.,and Takeda K.,2012,Construction of intraspecific linkage maps,detection of a chromosome inversion,and mapping of QTL for constitutive root aerenchyma formation in the teosinte Zea nicaraguensis,Mol.Breeding,29(1):137-146
Tase K.,and Kobayashi M.,1996,Evaluation of wintering ability in italian ryegrass(Lolium multittorum Lam)cultivars,J.JPNSoc.Hortic Sci.,42:63-67
Zaidi P.H.,Rashid Z.,Vinayan M.T.,Almeida G.D.,Phagna R.K.,and Babu R.,2015,QTL mapping of agronomic waterlogging tolerance using recombinant lines derived from tropical maize(Zea mays L.)germplasm,PLoS One,10(4):e0124350
Zhao J.R.,and Wang R.H.,2013,Development process,problem and countermeasure of maize production in China,Zhongguo Nongye Keji Daobao(Journal of Agricultural Science and Technology),15(3):1-6(赵久然,王荣焕,2013,中国玉米生产发展历程、存在问题及对策,中国农业科技导报,15(3):1-6)
Zhu J.X.,Su H.,Zeng X.,and Du H.W.,2017,The quality analysis of transcriptome responsive to waterlogging stress in maize,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(2):1-6(朱建雄,苏航,曾秀,杜何为,2017,玉米淹水胁迫转录组质量的分析,分子植物育种,15(2):1-6)