五味子、大枣、枸杞复合果醋配方设计及抗焦虑作用研究
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摘要
目的研究五味子、大枣、枸杞复合果醋(SCV)抗焦虑作用的最佳配方设计,并探讨其抗焦虑的作用机制.方法将ICR小鼠随机分为空白对照组(蒸馏水)、阳性对照组(地西泮)及SCV 9个不同配方组,各组小鼠连续灌服7 d后进行高架十字迷宫实验,并采用酶联免疫吸附法检测外周血中γ氨基丁酸和谷氨酸水平.结果高架十字迷宫实验结果显示:与空白对照组比较,SCV第7组小鼠进入开臂次数百分比明显增加(P<0.01),而第3,5,6,7,8及9组小鼠开臂停留时间百分比均明显增加(P<0.05或P<0.01).SCV第7组小鼠外周血中γ氨基丁酸水平明显升高,谷氨酸水平明显降低.结论 SCV抗焦虑最佳配方为100 m L SCV中含五味子果醋28 m L,大枣果醋7 m L,枸杞果醋28 m L,蒸馏水37 m L(各组果醋浓度为100%).此结果可为开发抗焦虑保健食品果醋提供依据.
Objective To obtain the optimal formulation design of Schisandra,Zizyphus and Lycium compound vinegar( SCV) and explore its anti-anxiety effects and the relative mechanism. Method ICR mice were randomly divided into three groups: blank control group( distilled water),positive control group( diazepam) and SCV group( including 9 different formula groups). After 7 days of continuous oral administration,the elevated plus maze test was performed and γ-aminobutyric acid( γGABA) and glutamine( Glu) in peripheral blood were detected by enzyme-linked immunosorbent assay. Results The elevated cross maze test showed that the percentage of open arm entry times in SCV group 7 was significantly higher than that in blank control group( P <0.01),while the percentage of open arm retention time in group 3,5,6,7,8 and 9 were significantly increased( P<0.05 or P<0.01).The level of γGABA in peripheral blood of SCV group 7 was significantly increased and glutamine level was decreased. Conclusion The optimal formula of SCV is as follows: 100 m L SCV containing Schisandra vinegar 28 m L,Zizyphus vinegar 7 m L,Lycium vinegar 28 m L,and distilled water 37 m L. This study provides the basis for the research and development of anti-anxiety health fruit vinegar.
Objective To obtain the optimal formulation design of Schisandra,Zizyphus and Lycium compound vinegar( SCV) and explore its anti-anxiety effects and the relative mechanism. Method ICR mice were randomly divided into three groups: blank control group( distilled water),positive control group( diazepam) and SCV group( including 9 different formula groups). After 7 days of continuous oral administration,the elevated plus maze test was performed and γ-aminobutyric acid( γGABA) and glutamine( Glu) in peripheral blood were detected by enzyme-linked immunosorbent assay. Results The elevated cross maze test showed that the percentage of open arm entry times in SCV group 7 was significantly higher than that in blank control group( P <0.01),while the percentage of open arm retention time in group 3,5,6,7,8 and 9 were significantly increased( P<0.05 or P<0.01).The level of γGABA in peripheral blood of SCV group 7 was significantly increased and glutamine level was decreased. Conclusion The optimal formula of SCV is as follows: 100 m L SCV containing Schisandra vinegar 28 m L,Zizyphus vinegar 7 m L,Lycium vinegar 28 m L,and distilled water 37 m L. This study provides the basis for the research and development of anti-anxiety health fruit vinegar.
引文
[1]刘永存,张波.失眠调节机制中西医研究进展[J].按摩与康复医学,2012,3(3):55-56.
[2]王雯雯,仰榴青,李永金,等.南、北五味子提取物对小鼠镇静、催眠作用的影响[J].江苏大学学报(医学版),2008,18(2):122-123,126.
[3]陈轶.黑果枸杞和白刺的抗焦虑作用及机制探讨[D].北京:北京中医药大学,2017.
[4]王雨艨.大枣中黄酮类成分研究进展[J].医药前沿,2013(9):332-333.
[5]滕俊,袁佳,叶莎莎.枸杞子化学成分及药理作用相关性概述[J].海峡药学,2014,26(6):36-37.
[6]张军武,邹文信,田凡,等.酸枣仁汤对高架十字迷宫所致焦虑大鼠的影响[J].西部中医药,2017,30(5):11-13.
[7]佟晴,袁永胜,徐勤荣,等.帕金森病患者血浆谷氨酸和γ氨基丁酸水平改变与睡眠障碍的关系[J].中华老年心脑血管病杂志,2015,17(8):823-825.
[8]李明泽,宋娟,张蕊,等.果醋功能性研究进展[J].江西农业,2018(12):57,64.
[9]李俊,袁灿兴,林秀凤,等.甘麦大枣汤及其不同加味对小鼠镇静催眠作用的比较[J].上海中医药杂志,2003,37(8):6-8.
[10]裘淼,熊中奎,吕梦宇.大枣多糖的药理作用研究进展[J].中国现代医生,2018,56(22):161-164.
[11]王维刚,吴文婷,周嘉斌,等.小鼠动物实验方法系列专题(五):应用高架十字迷宫分析小鼠焦虑行为[J].中国细胞生物学学报,2011,33(5):466-472.
[12]吴冰洁,董梅,李世平,等.不同环境对快速老化雄性小鼠抑郁与焦虑情绪的影响[J].中国神经精神疾病杂志,2018,44(8):476-481.
[2]王雯雯,仰榴青,李永金,等.南、北五味子提取物对小鼠镇静、催眠作用的影响[J].江苏大学学报(医学版),2008,18(2):122-123,126.
[3]陈轶.黑果枸杞和白刺的抗焦虑作用及机制探讨[D].北京:北京中医药大学,2017.
[4]王雨艨.大枣中黄酮类成分研究进展[J].医药前沿,2013(9):332-333.
[5]滕俊,袁佳,叶莎莎.枸杞子化学成分及药理作用相关性概述[J].海峡药学,2014,26(6):36-37.
[6]张军武,邹文信,田凡,等.酸枣仁汤对高架十字迷宫所致焦虑大鼠的影响[J].西部中医药,2017,30(5):11-13.
[7]佟晴,袁永胜,徐勤荣,等.帕金森病患者血浆谷氨酸和γ氨基丁酸水平改变与睡眠障碍的关系[J].中华老年心脑血管病杂志,2015,17(8):823-825.
[8]李明泽,宋娟,张蕊,等.果醋功能性研究进展[J].江西农业,2018(12):57,64.
[9]李俊,袁灿兴,林秀凤,等.甘麦大枣汤及其不同加味对小鼠镇静催眠作用的比较[J].上海中医药杂志,2003,37(8):6-8.
[10]裘淼,熊中奎,吕梦宇.大枣多糖的药理作用研究进展[J].中国现代医生,2018,56(22):161-164.
[11]王维刚,吴文婷,周嘉斌,等.小鼠动物实验方法系列专题(五):应用高架十字迷宫分析小鼠焦虑行为[J].中国细胞生物学学报,2011,33(5):466-472.
[12]吴冰洁,董梅,李世平,等.不同环境对快速老化雄性小鼠抑郁与焦虑情绪的影响[J].中国神经精神疾病杂志,2018,44(8):476-481.