摘要
目的研究五味子、大枣、枸杞复合果醋(SCV)抗焦虑作用的最佳配方设计,并探讨其抗焦虑的作用机制.方法将ICR小鼠随机分为空白对照组(蒸馏水)、阳性对照组(地西泮)及SCV 9个不同配方组,各组小鼠连续灌服7 d后进行高架十字迷宫实验,并采用酶联免疫吸附法检测外周血中γ氨基丁酸和谷氨酸水平.结果高架十字迷宫实验结果显示:与空白对照组比较,SCV第7组小鼠进入开臂次数百分比明显增加(P<0.01),而第3,5,6,7,8及9组小鼠开臂停留时间百分比均明显增加(P<0.05或P<0.01).SCV第7组小鼠外周血中γ氨基丁酸水平明显升高,谷氨酸水平明显降低.结论 SCV抗焦虑最佳配方为100 m L SCV中含五味子果醋28 m L,大枣果醋7 m L,枸杞果醋28 m L,蒸馏水37 m L(各组果醋浓度为100%).此结果可为开发抗焦虑保健食品果醋提供依据.
Objective To obtain the optimal formulation design of Schisandra,Zizyphus and Lycium compound vinegar( SCV) and explore its anti-anxiety effects and the relative mechanism. Method ICR mice were randomly divided into three groups: blank control group( distilled water),positive control group( diazepam) and SCV group( including 9 different formula groups). After 7 days of continuous oral administration,the elevated plus maze test was performed and γ-aminobutyric acid( γGABA) and glutamine( Glu) in peripheral blood were detected by enzyme-linked immunosorbent assay. Results The elevated cross maze test showed that the percentage of open arm entry times in SCV group 7 was significantly higher than that in blank control group( P <0.01),while the percentage of open arm retention time in group 3,5,6,7,8 and 9 were significantly increased( P<0.05 or P<0.01).The level of γGABA in peripheral blood of SCV group 7 was significantly increased and glutamine level was decreased. Conclusion The optimal formula of SCV is as follows: 100 m L SCV containing Schisandra vinegar 28 m L,Zizyphus vinegar 7 m L,Lycium vinegar 28 m L,and distilled water 37 m L. This study provides the basis for the research and development of anti-anxiety health fruit vinegar.
引文
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