卵巢癌患者外周血miR-31表达量与血清肿瘤标志物、基质金属蛋白酶的相关性研究
摘要
目的研究卵巢癌患者外周血miR-31表达量与血清肿瘤标志物、基质金属蛋白酶(MMPs)的相关性。方法选取2015年3月-2018年12月宁波市妇女儿童医院手术切除并经术后病理诊断的卵巢癌患者24例作为卵巢癌组,同期在该院体检的健康志愿者24例作为对照组。采集外周血及卵巢癌组织、癌旁组织,抽提miRNA后测定miR-31的表达量;采集血清并测定肿瘤标志物、MMPs的含量。结果卵巢癌组外周血中miR-31的表达量均明显低于对照组(P<0. 05),卵巢癌组织中miR-31的表达量均明显低于癌旁组织(P<0. 05),且卵巢癌患者外周血中miR-31的表达量与卵巢癌组织中miR-31的表达量呈正相关关系(P<0. 05)。卵巢癌组患者血清中CA50、CA125、CA199、组织多肽抗原(TPA)、MMP2、MMP7、MMP9、MMP10的含量均明显高于对照组(P<0. 05),且与外周血中miR-31的表达量呈负相关关系(P<0. 05)。结论卵巢癌患者外周血miR-31的低表达与病灶内miR-31表达量的下调相关且能反应肿瘤标志物、MMPs的变化。
引文
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[4]Hashemi ZS,Moghadam MF,Farokhimanesh S,et al.Inhibition of breast cancer metastasis by co-transfection of miR-31/193b-mimics[J].Iran J Basic Med Sci,2018,21(4):427-433.
[5]Zhong L,Huot J,Simard MJ.p38 activation induces production of miR-146a and miR-31 to repress E-selectin expression and inhibit transendothelial migration of colon cancer cells[J].Sci Rep,2018,8(1):2334.
[6]Wei J,Wang Z,Wang Z,et al.MicroRNA-31 function as a suppressor was regulated by epigenetic mechanisms in gastric cancer[J].Biomed Res Int,2017,2017:5348490.
[7]Kim B,Park Y,Kim B,et al.Diagnostic performance of CA125,HE4,and risk of ovarian malignancy algorithm for ovariancancer[J].J Clin Lab Anal,2018,15:e22624.
[8]Zhang W,Wang L,Xin Z.Combination of serum CA19-9 and CA125 levels and contrast-enhanced ultrasound parametric data facilitates to differentiate ovarian serous carcinoma from ovarian malignant epithelial cancer[J].Medicine(Baltimore),2018,97(16):e0358.
[9]Jabareen A,Abu-Jaafar A,Abou-Kandil A,et al.Effect of TPA and HTLV-1 Tax on BRCA1 and ERE controlled genes expression[J].Cell Cycle,2017,16(14):1336-1344.
[10]Kuasne H,Barros-Filho MC,Busso-Lopes A,et al.Integrative miRNA and mRNA analysis in penile carcinomas reveals markers and pathways with potential clinical impact[J].Oncotarget,2017,8(9):15294-15306.
[11]肖黎,张水蓉,胡萍,等.miRNA-31靶向基质金属蛋白酶-3基因对宫颈鳞癌侵袭力的影响[J].中国老年医学杂志,2018,38(9):2210-2212.
[12]Luddi A,Gori M,Marrocco C,et al.Matrix metalloproteinases and their inhibitors in human cumulus and granulosa cells as biomarkers for oocyte quality estimation[J].Fertil Steril,2018,109(5):930-939.
[13]Liu H,Zeng Z,Wang S,et al.Main components of pomegranate,ellagic acid and luteolin,inhibit metastasis of ovarian cancer by down-regulating MMP2 and MMP9[J].Cancer Biol Ther,2017,18(12):990-999.
[14]Ghasemi A,Hashemy SI,Aghaei M,et al.Leptin induces matrix metalloproteinase 7 expression to promote ovarian cancer cell invasion by activating ERK and JNK pathways[J].J Cell Biochem,2018,119(2):2333-2344.
[15]Mariya T,Hirohashi Y,Torigoe T,et al.Matrix metalloproteinase-10 regulates stemness of ovarian cancer stem-like cells by activation of canonical Wnt signaling and can be a target of chemotherapy-resistant ovarian cancer[J].Oncotarget,2016,7(18):26806-26822.