丹参酮抗小鼠辐射损伤实验研究
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摘要
目的探讨丹参酮的抗辐射损伤作用。方法建立紫外线损伤小鼠模型。取30只小鼠,随机分为6组,每组5只,即Ⅰ组(正常对照组,生理盐水),Ⅱ组(模型对照组),Ⅲ组(模型对照组,维生素C 5 g/L),Ⅳ～Ⅵ组为3个剂量丹参酮组(2. 5,5. 0,10. 0 g/L)。适应环境1周后,每日上午给予Ⅰ组和Ⅱ组0. 5 m L生理盐水灌胃,Ⅲ组0. 5 m L维生素C注射液灌胃,Ⅳ～Ⅵ组为0. 5 m L的各质量浓度梯度的丹参酮注射液灌胃;Ⅱ～Ⅵ组实验动物下午均接受10 min的紫外线照射,14 d后,取小鼠眼眶血并处死,采用比色法测定小鼠血清中谷胱甘肽还原酶(GR)、谷胱甘肽(GSH)、总抗氧化能力(T-AOC)、丙二醛(MDA)的活力或含量。结果与Ⅰ组相比,Ⅱ组小鼠的血清GR活力、T-AOC能力、GSH含量均明显下降,MDA含量明显上升(P <0. 05);与Ⅱ组相比,Ⅲ～Ⅵ组小鼠的血清GR活力、T-AOC能力、GSH含量均明显上升,MDA含量明显下降(P <0. 05);与Ⅳ～Ⅵ组相比,Ⅲ组小鼠的血清GR活力、T-AOC能力、GSH含量、MDA含量无显著差异(P> 0. 05)。结论丹参酮能清除氧自由基,降低紫外辐射对机体的损伤。
Objective To investigate the protective effect of tanshinone on radiation injury. Methods The mice models of ultraviolet injury were established. Totally 30 mice were randomly divided into 6 groups with 5 mice in each group: Group Ⅰ( normal control group,normal saline),group Ⅱ( model control group),group Ⅲ( model control group,5 g/L vitamin C),groups Ⅳ-Ⅵ( 2. 5,5. 0,10. 0 g/L tanshinone). One week after acclimatization to the environment,the mice in the group Ⅰ and groups Ⅱ received intragastric administration of 0. 5 m L normal saline daily in the morning,the mice in the group Ⅲ received intragastric administration of 0. 5 m L Vitamin C Injection,and the mice in the groups Ⅳ-Ⅵ received intragastric administration of 0. 5 m L Tanshinone Injection with different dosages. The mice in the groups Ⅱ-Ⅵreceived ultraviolet light for 10 min in the afternoon. After 14 d of treatment,the orbital blood of mice was taken and the mice were sacrificed,the activity or content of glutathione reductase( GR),glutathione( GSH),total antioxidant capacity( T-AOC) and malondialdehyde( MDA) in serum of mice were determined by colorimetry. Results Compared with group Ⅰ,the serum GR activity,T-AOC ability and GSH content of the mice in group Ⅱ decreased significantly,while the MDA content increased significantly( P < 0. 05). Compared with group Ⅱ,the serum GR activity,T-AOC ability and GSH content of the mice in groups Ⅲ-Ⅵ increased significantly,while the MDA content decreased significantly( P < 0. 05). There were no significant differences in serum GR activity,T-AOC ability,GSH content and MDA content among the groups Ⅳ-Ⅵ and group Ⅲ( P > 0. 05). Conclusion Tanshinone can eliminate oxygen free radicals and reduce the injury of ultraviolet radiation to the body.
Objective To investigate the protective effect of tanshinone on radiation injury. Methods The mice models of ultraviolet injury were established. Totally 30 mice were randomly divided into 6 groups with 5 mice in each group: Group Ⅰ( normal control group,normal saline),group Ⅱ( model control group),group Ⅲ( model control group,5 g/L vitamin C),groups Ⅳ-Ⅵ( 2. 5,5. 0,10. 0 g/L tanshinone). One week after acclimatization to the environment,the mice in the group Ⅰ and groups Ⅱ received intragastric administration of 0. 5 m L normal saline daily in the morning,the mice in the group Ⅲ received intragastric administration of 0. 5 m L Vitamin C Injection,and the mice in the groups Ⅳ-Ⅵ received intragastric administration of 0. 5 m L Tanshinone Injection with different dosages. The mice in the groups Ⅱ-Ⅵreceived ultraviolet light for 10 min in the afternoon. After 14 d of treatment,the orbital blood of mice was taken and the mice were sacrificed,the activity or content of glutathione reductase( GR),glutathione( GSH),total antioxidant capacity( T-AOC) and malondialdehyde( MDA) in serum of mice were determined by colorimetry. Results Compared with group Ⅰ,the serum GR activity,T-AOC ability and GSH content of the mice in group Ⅱ decreased significantly,while the MDA content increased significantly( P < 0. 05). Compared with group Ⅱ,the serum GR activity,T-AOC ability and GSH content of the mice in groups Ⅲ-Ⅵ increased significantly,while the MDA content decreased significantly( P < 0. 05). There were no significant differences in serum GR activity,T-AOC ability,GSH content and MDA content among the groups Ⅳ-Ⅵ and group Ⅲ( P > 0. 05). Conclusion Tanshinone can eliminate oxygen free radicals and reduce the injury of ultraviolet radiation to the body.
引文
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[12]李泽,孟哲,李宇娜,等.丹参酮ⅡA磺酸钠对血管紧张素Ⅱ诱导的大鼠心肌纤维化的影响[J].中国现代医学杂志,2018,28(12):17-23.
[13]高文强,张立进,张士伟,等.丹参酮Ⅰ对放射性膀胱炎的保护作用研究[J].现代泌尿外科杂志,2017,22(7):541-546.
[2]张玉方,赵春景.丹参酮ⅡA及其钠盐的药理作用研究进展[J].中国药业,2008,17(1):1-2.
[3]王秀月,赵川,李文静,等.丹参酮ⅡA抗结肠癌作用机制研究进展[J].中国临床药理学杂志,2018,34(5):597-599.
[4]张笑菲,高卓维,吕志平,等.丹参酮ⅡA抑制肝纤维化作用机制的研究进展[J].山东医药,2018,58(28):86-89.
[5]李双拾,耿秀娟,远丽娜.丹参多酚酸盐对慢性阻塞性肺疾病急性加重患者炎性反应及氧化应激的影响[J].中国药业,2016,25(12):51-54.
[6]王诗萌,李甜,杨田野,等.紫外线照射实验动物的皮肤光老化模型研究进展[J].中国美容医学,2018,27(7):146-150.
[7]吴斯敏,杨慧玲.紫外线引起皮肤光老化机制及防治的研究进展[J].医学综述,2018,24(2):341-346.
[8] LUSHCHAK VI. Free Radicals,Reactive Oxygen Species,Oxidative Stress and its Classification[J]. Chemico-Biological Interactions,2014,224:164-175.
[9]李倩,李焱,周铜水.新鲜丹参根酸水解产物及其抗氧化活性研究[J].安徽医药,2017,21(10):1785-1789.
[10] SHEN,WL,FEI Y,LU X,et al. Protective effects of tanshinoneⅡA derivative on myocardial ischemia/reperfusion injury in rats[J]. Journal of Chinese Pharmaceutical Sciences,2018,27(1):1-13.
[11]曹慧敏,吴瑾,贾连群,等.丹参酮ⅡA对心血管系统药理作用的研究进展[J].世界中医药,2017,12(7):1718-1722.
[12]李泽,孟哲,李宇娜,等.丹参酮ⅡA磺酸钠对血管紧张素Ⅱ诱导的大鼠心肌纤维化的影响[J].中国现代医学杂志,2018,28(12):17-23.
[13]高文强,张立进,张士伟,等.丹参酮Ⅰ对放射性膀胱炎的保护作用研究[J].现代泌尿外科杂志,2017,22(7):541-546.