生长调节剂处理的洒金柏扦插不定根解剖学研究
|
摘要
采用不同浓度的m(吲哚丁酸)∶m(萘乙酸)=1∶1溶液浸泡洒金柏1年生半木质化插穗,研究各处理对洒金柏1年生半木质化插穗扦插的影响,并采用优化的石蜡切片技术研究不定根发育过程中插穗的外部形态及组织结构变化。结果表明:80 mg/L的m_(吲哚丁酸):m_(萘乙酸)=1∶1溶液浸泡4 h处理的插穗的生根率最佳,为91.38%。洒金柏扦插不定根形成过程可划分为愈伤形成期、诱导期、表达期、伸长期4个连续时期,历时90 d左右。洒金柏不定根原基属于诱生根原基,扦插前插穗内部没有潜伏根原基;愈伤组织是不定根形成、生长和发育的基础,必须经过诱导才能形成,属于愈伤诱导生根型。不定根原基来源于愈伤组织和髓射线与形成层交叉处,属于多位点发生。
One-year-old semi-lignified cutting of Platycladus orientalis were immersed in different concentrations of m_(IBA)∶ m_(NAA)=1∶1, and the effects of each treatment on 1-year-old semi-lignified cutting of P. orientalis were studied. Improved paraffin section technique was used to study the changes in the external morphology and tissue structure of the cuttings during the development of adventitious roots, which will lay the foundation for the study of the cytological mechanism of adventitious root development. The results show that the best rooting rate of cuttings treat with 80 mg/L m_(IBA)∶m_(NAA) =1∶1 for 4 h is 91.38%. Morphological observations reveals that the formation process of adventitious roots could consist of 4 consecutive periods of callus formation,induction, expression, and elongation, which lasts for about 90 days. Anatomical observations show that the root primordium belongs to the induced root primordium. There are no latent roots in the cuttings before cuttings. And callus forms the basis for the formation, growth and development of adventitious roots. It must be induced to form callus before rooting, and belongs to callus induction type. The adventitious root primordium originated from the intersection of the callus and the myelin rays with the formation layer, belonging to multiple sites to take root.
One-year-old semi-lignified cutting of Platycladus orientalis were immersed in different concentrations of m_(IBA)∶ m_(NAA)=1∶1, and the effects of each treatment on 1-year-old semi-lignified cutting of P. orientalis were studied. Improved paraffin section technique was used to study the changes in the external morphology and tissue structure of the cuttings during the development of adventitious roots, which will lay the foundation for the study of the cytological mechanism of adventitious root development. The results show that the best rooting rate of cuttings treat with 80 mg/L m_(IBA)∶m_(NAA) =1∶1 for 4 h is 91.38%. Morphological observations reveals that the formation process of adventitious roots could consist of 4 consecutive periods of callus formation,induction, expression, and elongation, which lasts for about 90 days. Anatomical observations show that the root primordium belongs to the induced root primordium. There are no latent roots in the cuttings before cuttings. And callus forms the basis for the formation, growth and development of adventitious roots. It must be induced to form callus before rooting, and belongs to callus induction type. The adventitious root primordium originated from the intersection of the callus and the myelin rays with the formation layer, belonging to multiple sites to take root.
引文
[1]孙仲序,张金英,李云荣.洒金柏和千头柏性状的遗传和变异[J].山东农业大学学报, 1994, 25(1):105-111.
[2]梁荣纳,沈熙环.侧柏不同种源扦插繁殖的研究[J].北京林业大学学报, 1989, 11(4):47-52.
[3]魏黔春.侧柏古树扦插繁殖技术与生根机理研究[D].北京:中国林业科学研究院, 2015.
[4]李和平.植物显微技术:第二版[M].北京:科学出版社, 2016.
[5]权金娥,朱海兰,张春霞,等.四倍体刺槐茎段组织石蜡切片的制作方法[J].西北林学院学报, 2014, 29(3):140-144.
[6]张焕欣,董春娟,李福凯,等.植物不定根发生机理的研究进展[J].西北植物学报, 2017, 37(7):1457-1464.
[7]周新华,姚甲宝,肖智勇,等.插条尺寸和植物生长调节剂对杉木扦插生根的影响[J].西南林业大学学报(自然科学), 2018, 38(5):52-57.
[8]史锋厚,郑晨,罗帅,等.南京椴嫩枝扦插技术研究[J].中南林业科技大学学报, 2017, 37(8):6-10.
[9]徐笑玥,佟兆庆,闫文涛,等.中华金叶榆嫩枝扦插繁殖技术[J].东北林业大学学报, 2016, 44(5):1-4.
[10]周启贵,陈锡娟,汤春雁,等. NAA和IBA处理对罗汉松插条生长及生理生化的影响[J].西南大学学报,2017, 39(2):28-33.
[11]张俊鹏,查贵生,段安安,等.不同基质和基肥对旱冬瓜扦插及扦插苗移植的影响[J].西南林业大学学报(自然科学),2018,38(4):83-88.
[12]孙敬爽,郑红娟,贾桂霞,等.不同基质、生长调节剂、插穗规格和代谢调节剂对‘蓝星’扦插生根的影响[J].北京林业大学学报,2008,30(1):67-73.
[13]Trueman S J.Cytokinin and auxin effects on survival and rooting of Eucalyptus pellita and E.grandis×E.pellita cuttings[J].Rhizosphere,2018,6:74-76.
[14]杜伟,程嘉翎.桑树绿枝扦插皮部生根过程的形态解剖学观察[J].蚕业科学,2014,40(1):13-17.
[15]金建邦,祝遵凌,林庆梅.欧洲鹅耳枥扦插生根及解剖特性[J].西北农林科技大学学报(自然科学版),2015,43(1):92-98.
[16]朱聃,吕凤霞,陶洪波,等.矮丛越橘扦插生根的形态解剖学研究[J].东北农业大学学报,2011,42(7):413-146.
[17]Ikeuchi M,Iwase A,Rymen B,et al.Wounding triggers callus formation via dynamic hormonal and transcriptional changes[J].Plant Physiology,2017,175(3):1158-1174.
[18]张钢民,杨文利,贾玉彬,等.矮紫杉插条生根的解剖研究[J].园艺学报,1999,26(3):201-203.
[19]赵志新,李景云,李俊涛,等.白桦嫩枝扦插不定根形成的解剖观察[J].东北林业大学学报,1996,24(3):15-19.
[20]Liu J,Sheng L,Xu Y,et al.WOX11 and 12 are involved in the first-step cell fate transition during de novo root organogenesis in Arabidopsis[J].Plant Cell,2014,26(3):1081-1094.
[21]Porfírio S,Silva G D,Cabrita M J,et al.Reviewing current knowledge on olive(Olea europaea L.)adventitious root formation[J].Scientia Horticulturae,2016,198(26):207-226.
[22]Bryant P H,Trueman S J.Stem anatomy and adventitious root formation in cuttings of Angophora,Corymbia and Eucalyptus[J].Forests,2015,6(4):1227-1238.
[23]刘卫东,万朝瑶,饶龙兵,等.按树扦插生根的解剖学研究[J].中南林学院学报,1997,17(4):32-36.
[24]鲁丹,张瑞,彭方仁,等.红桤木扦插繁殖技术及生根过程的解剖学观察[J].西南林业大学学报,2013,33(2):24-28.
[25]王戈戎,袁晓颖.喜树茎解剖构造及插条不定根的形成[J].东北林业大学学报,2007,35(3):88-89.
[26]张晓平,方炎明.杂种鹅掌楸插穗不定根发生与发育的解剖学观察[J].植物资源与环境学报,2003,12(1):10-15.
[27]周祥明,刘玉堂,赵宪争,等.合欢硬枝扦插生根解剖及相关酶活性变化研究[J].植物研究,2016,36(1):8-61.
[2]梁荣纳,沈熙环.侧柏不同种源扦插繁殖的研究[J].北京林业大学学报, 1989, 11(4):47-52.
[3]魏黔春.侧柏古树扦插繁殖技术与生根机理研究[D].北京:中国林业科学研究院, 2015.
[4]李和平.植物显微技术:第二版[M].北京:科学出版社, 2016.
[5]权金娥,朱海兰,张春霞,等.四倍体刺槐茎段组织石蜡切片的制作方法[J].西北林学院学报, 2014, 29(3):140-144.
[6]张焕欣,董春娟,李福凯,等.植物不定根发生机理的研究进展[J].西北植物学报, 2017, 37(7):1457-1464.
[7]周新华,姚甲宝,肖智勇,等.插条尺寸和植物生长调节剂对杉木扦插生根的影响[J].西南林业大学学报(自然科学), 2018, 38(5):52-57.
[8]史锋厚,郑晨,罗帅,等.南京椴嫩枝扦插技术研究[J].中南林业科技大学学报, 2017, 37(8):6-10.
[9]徐笑玥,佟兆庆,闫文涛,等.中华金叶榆嫩枝扦插繁殖技术[J].东北林业大学学报, 2016, 44(5):1-4.
[10]周启贵,陈锡娟,汤春雁,等. NAA和IBA处理对罗汉松插条生长及生理生化的影响[J].西南大学学报,2017, 39(2):28-33.
[11]张俊鹏,查贵生,段安安,等.不同基质和基肥对旱冬瓜扦插及扦插苗移植的影响[J].西南林业大学学报(自然科学),2018,38(4):83-88.
[12]孙敬爽,郑红娟,贾桂霞,等.不同基质、生长调节剂、插穗规格和代谢调节剂对‘蓝星’扦插生根的影响[J].北京林业大学学报,2008,30(1):67-73.
[13]Trueman S J.Cytokinin and auxin effects on survival and rooting of Eucalyptus pellita and E.grandis×E.pellita cuttings[J].Rhizosphere,2018,6:74-76.
[14]杜伟,程嘉翎.桑树绿枝扦插皮部生根过程的形态解剖学观察[J].蚕业科学,2014,40(1):13-17.
[15]金建邦,祝遵凌,林庆梅.欧洲鹅耳枥扦插生根及解剖特性[J].西北农林科技大学学报(自然科学版),2015,43(1):92-98.
[16]朱聃,吕凤霞,陶洪波,等.矮丛越橘扦插生根的形态解剖学研究[J].东北农业大学学报,2011,42(7):413-146.
[17]Ikeuchi M,Iwase A,Rymen B,et al.Wounding triggers callus formation via dynamic hormonal and transcriptional changes[J].Plant Physiology,2017,175(3):1158-1174.
[18]张钢民,杨文利,贾玉彬,等.矮紫杉插条生根的解剖研究[J].园艺学报,1999,26(3):201-203.
[19]赵志新,李景云,李俊涛,等.白桦嫩枝扦插不定根形成的解剖观察[J].东北林业大学学报,1996,24(3):15-19.
[20]Liu J,Sheng L,Xu Y,et al.WOX11 and 12 are involved in the first-step cell fate transition during de novo root organogenesis in Arabidopsis[J].Plant Cell,2014,26(3):1081-1094.
[21]Porfírio S,Silva G D,Cabrita M J,et al.Reviewing current knowledge on olive(Olea europaea L.)adventitious root formation[J].Scientia Horticulturae,2016,198(26):207-226.
[22]Bryant P H,Trueman S J.Stem anatomy and adventitious root formation in cuttings of Angophora,Corymbia and Eucalyptus[J].Forests,2015,6(4):1227-1238.
[23]刘卫东,万朝瑶,饶龙兵,等.按树扦插生根的解剖学研究[J].中南林学院学报,1997,17(4):32-36.
[24]鲁丹,张瑞,彭方仁,等.红桤木扦插繁殖技术及生根过程的解剖学观察[J].西南林业大学学报,2013,33(2):24-28.
[25]王戈戎,袁晓颖.喜树茎解剖构造及插条不定根的形成[J].东北林业大学学报,2007,35(3):88-89.
[26]张晓平,方炎明.杂种鹅掌楸插穗不定根发生与发育的解剖学观察[J].植物资源与环境学报,2003,12(1):10-15.
[27]周祥明,刘玉堂,赵宪争,等.合欢硬枝扦插生根解剖及相关酶活性变化研究[J].植物研究,2016,36(1):8-61.