摘要
以8-羟基喹啉为络合剂,采用荧光分光光度法测定钙制剂中微量铝(Ⅲ),考察铝(Ⅲ)与8-羟基喹啉的显色条件.在pH=6. 0的醋酸-醋酸铵缓冲溶液中,铝(Ⅲ)与8-羟基喹啉形成稳定的配合物.使用三氯甲烷萃取,配合物的激发波长和发射波长分别为392 nm和512 nm.铝(Ⅲ)在2~200μg/L范围内,荧光强度与铝(Ⅲ)含量具有良好的线性关系,相关系数R~2=0. 998 3;方法的最低检出限为0. 2μg/L.该方法准确,简便,灵敏度高,可用于钙试剂中铝(Ⅲ)的测定.
In this paper,8-hydroxyquinoline as complexing agent and trichloromethane as extracting solvent were used to determine trace aluminium(Ⅲ) in calcium reagent by fluorescence spectrophotometry.The chromogenic conditions of aluminum(Ⅲ) and 8-hydroxyquinoline were investigated. In the acetic acid-ammonium acetate buffer solution of pH = 6. 0,stable complexes was formed by aluminum(Ⅲ) and 8-hydroxyquinolinol. Chloroform was used as extraction agent. The excitation and emission wavelengths of complexes were 392 nm and 512 nm,respectively. The fluorescence intensity of aluminum(Ⅲ) has a good linear relationship with the content of aluminum(Ⅲ) in the range of 2 ~ 200 g/L. The correlation coefficient R~2= 0. 998 3. The method is accurate,simple and sensitive,and it can be used for the determination of aluminum(Ⅲ) in calcium preparations.
引文
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