GP73和AFP联合检测在原发性肝癌诊断中的价值
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摘要
目的探讨血清高尔基体蛋白73(GP73)和甲胎蛋白(AFP)联合检测对原发性肝癌(PLC)诊断的临床价值。方法收集568例血清,其中原发性肝癌192例,慢性肝炎和肝硬化(CH/LC)患者256例,健康对照120例,采用酶联免疫吸附法检测各组GP73的水平,采用电化学发光法检测血清中AFP的水平,然后进行统计分析。结果 PLC组与CH/LC组和正常对照组的GP73含量和AFP含量的差异有统计学意义(P <0.05),GP73在PLC组的敏感性和特异性分别为61.45%和81.68%,AFP在PLC组的敏感性与特异性分别为42.19%和70.64%。GP73和AFP联合检测时敏感性与特异性分别为88.28%和43.16%。结论血清GP73联合AFP检测能够有效地提高PLC的诊断效率,可用于PLC高危人群的普查。
Objective To investigate the clinical significance of combined detection of serum golgi protein 73(GP73) and alpha fetoprotein(AFP) in the diagnosis of primary liver cancer(PLC). Methods A total of 568 serum cases were collected, including 192 cases of PLC, 256 cases of chronic hepatitis and cirrhosis(CH/LC), and 120 cases of healthy control. The level of GP73 in each group was detected by enzyme-linked immunosorbent assay. The level of AFP in serum was detected by electrochemiluminescence. The data were then statistically analyzed. Results The serum level of GP73 and AFP in PLC group was significantly higher than that in CH/LC and normal control group(P < 0.05), and the serum level of GP73 and AFP in CH/LC group was significantly higher than that in normal control group(P < 0.05). The sensitivity and specificity of GP73 in the PLC group were 61.45% and 81.68%, respectively. The sensitivity and specificity of AFP in the PLC group were 42.19% and 70.64%, respectively. The sensitivity and specificity of GP73 and AFP combination detection in the PLC group were 88.28% and 43.16%, respectively. Conclusion Combination detection of serum GP73 and AFP can effectively improve the diagnostic efficiency of PLC, and may be used for the screening of high-risk group of PLC.
Objective To investigate the clinical significance of combined detection of serum golgi protein 73(GP73) and alpha fetoprotein(AFP) in the diagnosis of primary liver cancer(PLC). Methods A total of 568 serum cases were collected, including 192 cases of PLC, 256 cases of chronic hepatitis and cirrhosis(CH/LC), and 120 cases of healthy control. The level of GP73 in each group was detected by enzyme-linked immunosorbent assay. The level of AFP in serum was detected by electrochemiluminescence. The data were then statistically analyzed. Results The serum level of GP73 and AFP in PLC group was significantly higher than that in CH/LC and normal control group(P < 0.05), and the serum level of GP73 and AFP in CH/LC group was significantly higher than that in normal control group(P < 0.05). The sensitivity and specificity of GP73 in the PLC group were 61.45% and 81.68%, respectively. The sensitivity and specificity of AFP in the PLC group were 42.19% and 70.64%, respectively. The sensitivity and specificity of GP73 and AFP combination detection in the PLC group were 88.28% and 43.16%, respectively. Conclusion Combination detection of serum GP73 and AFP can effectively improve the diagnostic efficiency of PLC, and may be used for the screening of high-risk group of PLC.
引文
[1]Ge JB,Xu YJ.Internal medicine.8th ed.Beijing:People's Medical Publishing House,2013:429.(in Chinese)葛均波,徐永健.内科学.8版.北京:人民卫生出版社,2013:429.
[2]Zhu B,Xiao YX,Peng YS,et al.GP73,GPC3 and AFP combined detection in the diagnosis of primary liver cancer.Chongqing Med,2016,45(10):1367-1369.(in Chinese)朱波,肖亚雄,彭宇生,等.GP73、GPC3和AFP联合检测在原发性肝癌诊断中的探讨.重庆医学,2016,45(10):1367-1369.
[3]Xu QM,Chen H,Duan Z,et al.Dignostic value of GP73 combined with AFP,GGT-II detection on primary liver cancer.Mod Med J China,2014,16(9):23-26.(in Chinese)许奇美,陈辉,段智,等.GP73联合AFP及GGT-II检测对原发性肝癌的诊断价值.中国现代医药杂志,2014,16(9):23-26.
[4]Chen YB,Mo CY.Diagnosis value of serum AFP,GGT-II,GP73 for primary hepatic carcinoma.Chin J Lab Diagn,2016,20(8):1310-1312.(in Chinese)陈贻斌,莫翠毅.原发性肝癌患者血清AFP、GGT II和GP73检测的临床意义.中国实验诊断学,2016,20(8):1310-1312.
[5]Cui RL,Li YG,Zhang PY,et al.Combined detection of alpha-fetoprotein and Golgi protein 73 for primary hepatic carcinoma diagnosis.Chin J Clin Oncol,2015,42(4):222-225.(in Chinese)崔冉亮,李悦国,张鹏宇,等.血清高尔基体蛋白73联合甲胎蛋白检测在原发性肝癌患者中的诊断价值.中国肿瘤临床,2015,42(4):222-225.
[6]Xiao L,Mao R,Yang Y,et al.The clinical value of single and combined detection by golgi protein 73,α-L-fucosidase,andα-fetoprotein in the diagnosis of primary hepatic carcinoma.Chin Gen Pract,2013,16(14):1581-1583.(in Chinese)肖蕾,毛睿,杨颖,等.高尔基体糖蛋白73、α-L-岩藻糖苷酶、甲胎蛋白单项检测与联合检测对原发性肝癌的诊断价值研究.中国全科医学,2013,16(14):1581-1583.
[7]Zhu QH.Diagnostic value of joint detection of golgin 73,alphafetoprotein,alpha-fetoprotein variants for primary hepatocellular carcinoma.China Med Herald,2016,13(5):130-133.(in Chinese)诸琴红.高尔基体蛋白73、甲胎蛋白、甲胎蛋白异质体3%联合检测对原发性肝癌的诊断价值.中国医药导报,2016,13(5):130-133.
[8]Block TM,Comunale MA,Lowman M,et a1.Use of targeted glycoproteomics to identify serum glycoproteins that correlate with liver cancer in woodchucks and humans.Proc Natl Acad Sci U S A,2005,102(6):779-784.
[9]Shi Y,Chen J,Li L,et al.A study of diagnostic value of golgi protein GP73 and its genetic assay in primary hepatic carcinoma.Technol Cancer Res Treat,2011,10(3):287-294.
[2]Zhu B,Xiao YX,Peng YS,et al.GP73,GPC3 and AFP combined detection in the diagnosis of primary liver cancer.Chongqing Med,2016,45(10):1367-1369.(in Chinese)朱波,肖亚雄,彭宇生,等.GP73、GPC3和AFP联合检测在原发性肝癌诊断中的探讨.重庆医学,2016,45(10):1367-1369.
[3]Xu QM,Chen H,Duan Z,et al.Dignostic value of GP73 combined with AFP,GGT-II detection on primary liver cancer.Mod Med J China,2014,16(9):23-26.(in Chinese)许奇美,陈辉,段智,等.GP73联合AFP及GGT-II检测对原发性肝癌的诊断价值.中国现代医药杂志,2014,16(9):23-26.
[4]Chen YB,Mo CY.Diagnosis value of serum AFP,GGT-II,GP73 for primary hepatic carcinoma.Chin J Lab Diagn,2016,20(8):1310-1312.(in Chinese)陈贻斌,莫翠毅.原发性肝癌患者血清AFP、GGT II和GP73检测的临床意义.中国实验诊断学,2016,20(8):1310-1312.
[5]Cui RL,Li YG,Zhang PY,et al.Combined detection of alpha-fetoprotein and Golgi protein 73 for primary hepatic carcinoma diagnosis.Chin J Clin Oncol,2015,42(4):222-225.(in Chinese)崔冉亮,李悦国,张鹏宇,等.血清高尔基体蛋白73联合甲胎蛋白检测在原发性肝癌患者中的诊断价值.中国肿瘤临床,2015,42(4):222-225.
[6]Xiao L,Mao R,Yang Y,et al.The clinical value of single and combined detection by golgi protein 73,α-L-fucosidase,andα-fetoprotein in the diagnosis of primary hepatic carcinoma.Chin Gen Pract,2013,16(14):1581-1583.(in Chinese)肖蕾,毛睿,杨颖,等.高尔基体糖蛋白73、α-L-岩藻糖苷酶、甲胎蛋白单项检测与联合检测对原发性肝癌的诊断价值研究.中国全科医学,2013,16(14):1581-1583.
[7]Zhu QH.Diagnostic value of joint detection of golgin 73,alphafetoprotein,alpha-fetoprotein variants for primary hepatocellular carcinoma.China Med Herald,2016,13(5):130-133.(in Chinese)诸琴红.高尔基体蛋白73、甲胎蛋白、甲胎蛋白异质体3%联合检测对原发性肝癌的诊断价值.中国医药导报,2016,13(5):130-133.
[8]Block TM,Comunale MA,Lowman M,et a1.Use of targeted glycoproteomics to identify serum glycoproteins that correlate with liver cancer in woodchucks and humans.Proc Natl Acad Sci U S A,2005,102(6):779-784.
[9]Shi Y,Chen J,Li L,et al.A study of diagnostic value of golgi protein GP73 and its genetic assay in primary hepatic carcinoma.Technol Cancer Res Treat,2011,10(3):287-294.