响应面优化济生散蛋白水提工艺及氨基酸测定
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摘要
目的:优化由牛肉、牛筋腱、猪肤、大枣肉组成的济生散蛋白提取工艺,检测有效成分。方法:采用响应面法优化蛋白提取工艺,紫外-分光光度法、凯氏定氮法进行成分分析及检测。结果:水煮时间25.38 min、保温温度71.07℃、保温时间12.02 h时提取物中蛋白含量最高,且在处方量提取物中检测出赖氨酸(435.88±1.62)μg、色氨酸(86.20±2.51)μg、羟脯氨酸(2663.40±25.02)μg。结论:响应面优化济生散蛋白水提工艺数据稳定,重复性好,工艺方法简便易行,符合日常实际应用。
Extraction of active ingredients from a composition Jisheng powder to improve the concentration of HDL and the analysis of components were investigated in this paper. Response surface design was done to screen the best extraction technology. UV spectrophotometry and Kjeldah method were experimented to analysis the principal components. Experimental results demonstrated that the content of protein extraction was the highest when the boiling time was 25.38 min, the holding temperature was 71.07 ℃ and the holding time was 12.02 h. The extraction under the optimum technological condition was analyzed. Amino acids cover lysine(435.88±1.62)μg, tryptophan(86.20±2.51)μg and hydroxyproline(2663.40±25.02)μg. The data was reliable and the process was simple and easy and conformed to the daily practical application.
Extraction of active ingredients from a composition Jisheng powder to improve the concentration of HDL and the analysis of components were investigated in this paper. Response surface design was done to screen the best extraction technology. UV spectrophotometry and Kjeldah method were experimented to analysis the principal components. Experimental results demonstrated that the content of protein extraction was the highest when the boiling time was 25.38 min, the holding temperature was 71.07 ℃ and the holding time was 12.02 h. The extraction under the optimum technological condition was analyzed. Amino acids cover lysine(435.88±1.62)μg, tryptophan(86.20±2.51)μg and hydroxyproline(2663.40±25.02)μg. The data was reliable and the process was simple and easy and conformed to the daily practical application.
引文
[1]杜海燕,方振威,林阳.他汀类药物防治动脉粥样硬化性心血管疾病相关指南解读及其临床应用现状分析[J].中国医院用药评价与分析,2015,(2):148-153
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[10]田迪,谭迎,郭志刚.高密度脂蛋白亚组分的分离和检测方法及其临床意义[J].心血管病学进展,2011,32(6):795-798
[11]Di Lullo Gloria A,Sweeney Shawn M,Korkko Jarmo,et al.Mapping the ligand-binding sites and diseaseassociated mutations on the most abundant protein in the human,type I collagen[J].Journal of Biological Chemistry,2002,277(6):4223-4231
[2]虞燕霞,陈伯文,尚尔宁,等.调节高密度脂蛋白药物的研究进展[J].中国药理学与毒理学杂志,2010,24(4):311-316
[3]高庄存,高翔宇.显著升高血清高密度脂蛋白浓度的中药复合组方和制备方法[P].中国专利:CN:103405538,2013-11-27
[4]王孝平,邢树礼.考马斯亮蓝法测定蛋白含量的研究[J].天津化工,2009,23(3):40-41
[5]王冬燕,王远红,郭丽萍,等.纳豆中氨基酸态氮含量的测定[J].食品工业科技,2010,29(9):361-366
[6]张俊杰,曾庆孝.比色法测定鱼鳞中羟脯氨酸的研究[J].食品科技,2004,30(4):83-85
[7]李桂玲,李欢庆,刘从彬.谷物中赖氨酸含量测定方法的探析[J].河南工业大学学报,2006,27(5):65-80
[8]斯钦,巴图,茹水鲜,等.酶水解比色法测定奶液中色氨酸含量的研究[J].内蒙古师大学报,1998,27(3):203-205
[9]魏永义,赵国品,张莉,等.凯氏定氮法测定火腿肠中的蛋白质[J].肉类工业,2011,(4):16-18
[10]田迪,谭迎,郭志刚.高密度脂蛋白亚组分的分离和检测方法及其临床意义[J].心血管病学进展,2011,32(6):795-798
[11]Di Lullo Gloria A,Sweeney Shawn M,Korkko Jarmo,et al.Mapping the ligand-binding sites and diseaseassociated mutations on the most abundant protein in the human,type I collagen[J].Journal of Biological Chemistry,2002,277(6):4223-4231