摘要
以香菇为原料,研究香菇中多糖的最佳提取工艺。采用高效液相色谱法测定香菇多糖的含量,回收率为99.37%,RSD为0.11%。通过单因素试验优化工艺参数,结果表明:最佳提取条件为料液比1∶20 (g/mL),提取温度为110℃,提取时间为7 min,提取压力为7 MPa,提取次数为3次,提取功率为300 W。亚临界水提法可明显缩短提取时间和降低成本,且含量更高。
Taking lentinus edodes as raw material, the optimum extraction technology of polysaccharide from lentinus edodes was studied. The content of lentinan was determined by HPLC. The recovery rate was 99.37% and RSD was 0.11%. The technological parameters were optimized by single factor test. The results showed that the optimum extraction conditions were as follows: material-liquid ratio 1 ∶ 20(g/mL), extraction temperature 110 ℃, extraction time7 min, extraction pressure 7 MPa, extraction times 3 times, extraction power 300 W. Subcritical water extraction could obviously shorten the extraction time and reduce the cost, and the content was higher.
引文
[1]娄冠群,张永忠,李振艳,等.亚临界水提取香菇多糖的工艺研究[J].东北农业大学学报, 2010, 41(2):138-142.
[2]徐晓飞,陈健.多糖含量测定的研究进展[J].食品科学, 2009,30(21):443-448.
[3]邓辰辰,相继芬,董兴叶,等.亚临界水提取灵芝多糖的工艺研究[J].河南工业大学学报(自然科学版), 2016, 37(2):105-108.
[4]程斌.金针菇多糖的亚临界水萃取工艺研究[J].食品工业, 2015,36(7):47-50.
[5]张晓倩,王荣春,马莺,等.亚临界水技术及其在天然产物提取中的应用[J].食品研究与开发, 2015, 36(11):132-136.
[6]任红,乔洪翔,方玲,等. HPLC-ELSD测定银杏叶多糖组成及单糖含量[J].中国现代应用药学, 2015, 32(6):711-714.
[7]黄强.高效液相色谱法测定葡萄酒中白藜芦醇的含量[J].民营科技, 2013(11):93.