HPLC法结合统计学方法研究复方丹参片中皂苷类成分
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摘要
目的:建立复方丹参片中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量测定方法,并采用PCA和HCA法进行分析。方法:采用高效液相色谱法,采用AlltimaTM C_(18)(4.6mm×250mm,5μm)Serial No 209050188色谱柱;流动相:乙腈-水;梯度洗脱;检测波长203nm;柱温:30℃;流速:1mL/min;进样量:5μL。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1的线性范围分别为0.106～1.06μg(r=0.9994),0.533～5.33μg(r=0.9998),0.0524～0.524μg(r=0.9997),0.582～5.82μg(r=0.9999);平均加样回收率(n=9)分别为98.02%,98.29%,98.72%,99.77%,RSD分别为2.82%,3.59%,2.43%,2.95%。结论:PCA和HCA较全面的反映了复方丹参片样本的信息,评价结果具有一定的客观性,可为复方丹参片的质量控制提供参考。
Objective:To establish the assay method of notoginsenoside R1,ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 in Compound Danshen Tablets by HPLC and PCA and HCA were conducted for analysis.Methods:This assay was performed on AlltimaTM C_(18)(4.6 mm×250 mm,5 μm)Serial No 209050188 column with acetonitrile-water as mobile phase in gradient elution at a flow rate of 1 mL/min.The detection wavelength was 203 nm.The column temperature was 30 ℃.The injection volumn was 5 μL.Results:The liner rangers of notoginsenoside R1,ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 were 0.106-1.06 μg(r=0.9994),0.533-5.33 μg(r=0.9998),0.0524-0.524 μg(r=0.9997),0.582-5.82 μg(r=0.9999),respectively.Their average recoveries(n=9)were 98.02%,98.29%,98.72% and 99.77%,corresponding RSD values were 2.82%,3.59%,2.43% and 2.95%,respectively.Conclusion:The analysis of HCA and PCA could comprehensively reflect the samples of Compound Danshen Tablets.The results were objective,which can be applied to the quality control method of panax notoginseng in Compound Danshen Tablets effectively.
Objective:To establish the assay method of notoginsenoside R1,ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 in Compound Danshen Tablets by HPLC and PCA and HCA were conducted for analysis.Methods:This assay was performed on AlltimaTM C_(18)(4.6 mm×250 mm,5 μm)Serial No 209050188 column with acetonitrile-water as mobile phase in gradient elution at a flow rate of 1 mL/min.The detection wavelength was 203 nm.The column temperature was 30 ℃.The injection volumn was 5 μL.Results:The liner rangers of notoginsenoside R1,ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 were 0.106-1.06 μg(r=0.9994),0.533-5.33 μg(r=0.9998),0.0524-0.524 μg(r=0.9997),0.582-5.82 μg(r=0.9999),respectively.Their average recoveries(n=9)were 98.02%,98.29%,98.72% and 99.77%,corresponding RSD values were 2.82%,3.59%,2.43% and 2.95%,respectively.Conclusion:The analysis of HCA and PCA could comprehensively reflect the samples of Compound Danshen Tablets.The results were objective,which can be applied to the quality control method of panax notoginseng in Compound Danshen Tablets effectively.
引文
[1] 国家药典委员会.中华人民共和国药典(2015年版一部)[S].北京:化学工业出版社,2015.
[2] Qin Ren-an,Lin Juan,Li Chuyuan,et al.Study of the protective mechanisms of Compound Danshen Tablet (Fufang Danshen Pian) against myocardial ischemia/reperfusion injury via the Akt-eNOS signaling pathway in rats[J].Journal of Ethnophar-macology,2014,156(28):190-198.
[3] 余小平,张超群,覃仁安,等.复方丹参片对急性脑缺血大鼠血管新生因子影响的研究[J].中华中医药学刊,2011,28(5):1145-1148.
[4] 刘利娟,周德生,肖志杰,等.复方丹参片对阿尔茨海默病转基因细胞模型Tau蛋白表达的影响[J].湖南中医药大学学报,2014,34(5):14-17.
[5] Ren-an QIN,Xiao-xuan YAO,Zhu-ying HUANG.Effects of Compound Dans-hen tablets on spatial cognition and expression of brain β-amyloid precursorprotein in a rat model of alzheimer′s disease [J].Journal of Traditional Chinese Medicine,2012,32(1):63-66.
[6] 范彦俊,俊英.近五年复方丹参片的研究进展[J].齐鲁药事,2009,28(4):225-228.
[7] 冯中,李晓燕,刘波,等.HPLC测定不同厂家复方丹参片中三七皂苷R1和人参皂苷Re、Rg1、Rb1含量[J].中成药,2009,30(1):71-74.
[8] 蔡晓洋,张思荻,曾俊,等.基于主成分分析和聚类分析的栀子种质资源评价[J].中国实验方剂学杂志,2017,23(14):30-37.
[9] 孙振球.医学统计学[M].3版.北京:人民卫生出版社,2010:354.
[10] 张文彤.SPSS统计分析高级教程[M].北京:高等教育出版社,2004:213-233.
[2] Qin Ren-an,Lin Juan,Li Chuyuan,et al.Study of the protective mechanisms of Compound Danshen Tablet (Fufang Danshen Pian) against myocardial ischemia/reperfusion injury via the Akt-eNOS signaling pathway in rats[J].Journal of Ethnophar-macology,2014,156(28):190-198.
[3] 余小平,张超群,覃仁安,等.复方丹参片对急性脑缺血大鼠血管新生因子影响的研究[J].中华中医药学刊,2011,28(5):1145-1148.
[4] 刘利娟,周德生,肖志杰,等.复方丹参片对阿尔茨海默病转基因细胞模型Tau蛋白表达的影响[J].湖南中医药大学学报,2014,34(5):14-17.
[5] Ren-an QIN,Xiao-xuan YAO,Zhu-ying HUANG.Effects of Compound Dans-hen tablets on spatial cognition and expression of brain β-amyloid precursorprotein in a rat model of alzheimer′s disease [J].Journal of Traditional Chinese Medicine,2012,32(1):63-66.
[6] 范彦俊,俊英.近五年复方丹参片的研究进展[J].齐鲁药事,2009,28(4):225-228.
[7] 冯中,李晓燕,刘波,等.HPLC测定不同厂家复方丹参片中三七皂苷R1和人参皂苷Re、Rg1、Rb1含量[J].中成药,2009,30(1):71-74.
[8] 蔡晓洋,张思荻,曾俊,等.基于主成分分析和聚类分析的栀子种质资源评价[J].中国实验方剂学杂志,2017,23(14):30-37.
[9] 孙振球.医学统计学[M].3版.北京:人民卫生出版社,2010:354.
[10] 张文彤.SPSS统计分析高级教程[M].北京:高等教育出版社,2004:213-233.