高效液相色谱法同时测定山柰中槲皮素和山柰素含量
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摘要
目的建立高效液相色谱法测定山柰药材中槲皮素和山柰素的含量。方法采用ODS2 C18(5μm,4.6 mm×150 mm)色谱柱,以甲醇-0.4%磷酸(47∶53)为流动相,流速1 m L/min,柱温30℃,检测波长为367 nm,进样量为10μL。结果槲皮素在0.016 5~1.65μg范围内呈现良好的线性关系(r=0.999 7),平均回收率为96.8%,RSD=2.02%;山柰素在0.014 6~1.46μg范围内呈现良好的线性关系(r=0.999 5),平均回收率为97.3%,RSD=1.77%。结论该方法操作简单、结果准确、重复性较好,可用于山柰药材中槲皮素和山柰素的含量测定。
Objective To establish an HPLC method for the simultaneous determination of quercetin and kaempferol in Kaempferia galanga L.. Methods ODS2 C18(5 μm, 4.6 mm×150 mm) was used as chromatographic column; methanol-0.4% phosphate(47:53) was the mobile phase; the flow rate was 1 m L/min; column temperature was 30 ℃; the detection wavelength was 367 nm; the injection volume was 10 μL. Results Quercetin showed good linear relationship in the range of 0.016 5–1.65 μg(r=0.999 7). The average recovery rate was 96.8%, RSD=2.02%; kaempferol showed good linear relationship in the range of 0.014 6–1.46 μg(r=0.999 5). The average recovery rate was 97.3%, RSD=1.77%. Conclusion The method is simple, accurate, and with good reproducibility, which can be used for content determination of quercetin and kaempferol in Kaempferia galanga L..
Objective To establish an HPLC method for the simultaneous determination of quercetin and kaempferol in Kaempferia galanga L.. Methods ODS2 C18(5 μm, 4.6 mm×150 mm) was used as chromatographic column; methanol-0.4% phosphate(47:53) was the mobile phase; the flow rate was 1 m L/min; column temperature was 30 ℃; the detection wavelength was 367 nm; the injection volume was 10 μL. Results Quercetin showed good linear relationship in the range of 0.016 5–1.65 μg(r=0.999 7). The average recovery rate was 96.8%, RSD=2.02%; kaempferol showed good linear relationship in the range of 0.014 6–1.46 μg(r=0.999 5). The average recovery rate was 97.3%, RSD=1.77%. Conclusion The method is simple, accurate, and with good reproducibility, which can be used for content determination of quercetin and kaempferol in Kaempferia galanga L..
引文
[1]谭雄斯.HPLC-UUV-ELSD法测定益益肝乐颗粒中槲皮皮素、山柰素、柴胡皂苷a和柴胡皂苷d d[J].中成药,20114,36(3):531-535.
[2]李慧,许亮,徐保保利,等.HPLC测定定地锦草中没食子子酸、槲皮素及山柰素含含量[J].中国实验验方剂学杂志,2012,18(7):100-1102.
[3]杜小伟,王京辉辉,肖斯婷,等.高效效液相色谱法测定不同来源罗布麻叶中中槲皮素和山柰素素的含量[J].中国中医药信息杂志,2013,20(1):51-553.
[4]梁选革,张若燕燕,刘莉丽.红花注注射液中槲皮素和山柰素含量的同效液相相色谱测定[J].时时珍国医国药,2012,23(10):24755-2477.
[5]黄瑞松,雷沛霖霖,苏青,等.壮药茉茉莉花槲皮素和山柰素含量测定方法的的建立[J].广西医医科大学学报,2013,30(1):38-411.
[6]胡顺莉,俞致,吴吴虹,等.苦丁茶提提取物质量分析[J].安徽中医药大学学学报,2014,33(2):81-84.
[7]胡婧,王璐瑶,万万定荣,等.珠芽景景天抗肿瘤活性研研究及槲皮素和山柰素素的含量测定[J].中国实验方剂学学杂志,2013,19(224):139-142.
[8]国家药典委员会会.中华人民共和和国药典:一部[MM].北京:中国医药科技技出版社,2010:27.
[9]梁洁,余靓,柳贤贤福,等.HPLC法同时测定龙眼花中中3种黄酮类化合物含含量[J].世界科学学技术-中医药现现代化,2013,15(77):1576-1580.
[2]李慧,许亮,徐保保利,等.HPLC测定定地锦草中没食子子酸、槲皮素及山柰素含含量[J].中国实验验方剂学杂志,2012,18(7):100-1102.
[3]杜小伟,王京辉辉,肖斯婷,等.高效效液相色谱法测定不同来源罗布麻叶中中槲皮素和山柰素素的含量[J].中国中医药信息杂志,2013,20(1):51-553.
[4]梁选革,张若燕燕,刘莉丽.红花注注射液中槲皮素和山柰素含量的同效液相相色谱测定[J].时时珍国医国药,2012,23(10):24755-2477.
[5]黄瑞松,雷沛霖霖,苏青,等.壮药茉茉莉花槲皮素和山柰素含量测定方法的的建立[J].广西医医科大学学报,2013,30(1):38-411.
[6]胡顺莉,俞致,吴吴虹,等.苦丁茶提提取物质量分析[J].安徽中医药大学学学报,2014,33(2):81-84.
[7]胡婧,王璐瑶,万万定荣,等.珠芽景景天抗肿瘤活性研研究及槲皮素和山柰素素的含量测定[J].中国实验方剂学学杂志,2013,19(224):139-142.
[8]国家药典委员会会.中华人民共和和国药典:一部[MM].北京:中国医药科技技出版社,2010:27.
[9]梁洁,余靓,柳贤贤福,等.HPLC法同时测定龙眼花中中3种黄酮类化合物含含量[J].世界科学学技术-中医药现现代化,2013,15(77):1576-1580.