柱前衍生高效液相色谱法测定功能饮料中牛磺酸含量的条件优化
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摘要
目的 优化柱前衍生高效液相色谱条件检测功能饮料中牛磺酸的含量。方法 采用配备紫外检测器的高效液相色谱仪,利用C_18反相色谱柱,选择甲醇:乙酸钠=45:55(V:V)为流动相,在流速为0.8 mL/min、标液与衍生试剂比例为1:1(V:V)、衍生时间为1 h时进行检测。结果 在1.0～200μg/mL范围内,牛磺酸的浓度与峰面积呈现良好的线性,标准曲线方程是Y=34182X+14599,r~2=0.9999,优化后的方法回收率在96.83%～100.17%之间,检出限是0.53mg/100g,定量限为1.76mg/100g。结论 本方法高效、准确,适用于能饮料中牛磺酸含量的检测,为日常监管工作提供理论支持。
Objective To optimize the conditions of high performance liquid chromatography(HPLC) with precolumn derivatization for determination of taurine content in functional drinks. Methods The samples were determined by HPLC with ultraviolet detector, using C_18 reversed phase as chromatograph column. The mobile phase was methanol and sodium acetate(45:55, V:V), with the flow rate of 0.8 mL/min, the ratio of standard solution and derivatization of 1:1, and the derivatization time of 1 h. Results When the concentration of taurine ranged from 1.0 to 200 μg/mL, the concentration of taurine and peak area had a good correlation. The standard curve equation was Y=34182 X+14599, r~2=0.9999. The recoveries of the optimized method were 96.83%-100.17%. The limit of detection was 0.53 mg/100 g and the limit of quantitation was 1.76 mg/100 g. Conclusion The method is efficient and accurate, and is suitable for detecting the content of taurine in functional drinks, so as to provide theoretical support for daily supervision work.
Objective To optimize the conditions of high performance liquid chromatography(HPLC) with precolumn derivatization for determination of taurine content in functional drinks. Methods The samples were determined by HPLC with ultraviolet detector, using C_18 reversed phase as chromatograph column. The mobile phase was methanol and sodium acetate(45:55, V:V), with the flow rate of 0.8 mL/min, the ratio of standard solution and derivatization of 1:1, and the derivatization time of 1 h. Results When the concentration of taurine ranged from 1.0 to 200 μg/mL, the concentration of taurine and peak area had a good correlation. The standard curve equation was Y=34182 X+14599, r~2=0.9999. The recoveries of the optimized method were 96.83%-100.17%. The limit of detection was 0.53 mg/100 g and the limit of quantitation was 1.76 mg/100 g. Conclusion The method is efficient and accurate, and is suitable for detecting the content of taurine in functional drinks, so as to provide theoretical support for daily supervision work.
引文
[1]GB 16740-2014食品安全国家标准保健食品[S].GB 16740-2014 National food safety standard-Health food[S].
[2]陈秋虹,莫建光,黄艳.天然牛磺酸的提取与应用[J].氨基酸和生物资源,2011,33(2):43-45.Chen QH,Mo JG,Huang Y.Extraction and application of natural taurine[J].Amino Acids Biotic Res,2011,33(2):43-45.
[3]刘亚风,袁萍,祝伟霞,等.牛磺酸检测技术研究进展[J].中国卫生检验杂志,2010,20(7):1831-1833.Liu YF,Yuan P,Zhu WX,et al.Research progress of taurine detection technology[J].Chin J Health Lab Technol,2010,20(7):1831-1833.
[4]卢忠,罗一帆.中和滴定法测定牛磺酸含量[J].数理医药学杂志,2000,13(4):350.Lu Z,Luo YF.Determination of taurine by neutralization titration[J].JMathemat Med,2000,13(4):350.
[5]杨祖英,张平伟.薄层色谱法测定食品中牛磺酸[J].卫生研究,1996,25(4):248-250.Yang ZY,Zhang PW.Determination of taurine in food by thin layer chromatography[J].J Hyg Res,1996,25(4):248-250.
[6]王海澍.高效液相色谱法测定牡蛎中牛磺酸含量[J].食品安全导刊,2017,(3):90.Wang HS.Determination of taurine in oyster by HPLC[J].China Food Saf Magaz,2017,(3):90.
[7]孟冰冰,杨桂玲,徐惠,等.程序进样-柱前衍生-超高效液相色谱法测定鱼露酱油中的牛磺酸含量[J].中国调味品,2017,42(5):138-140.Meng BB,Yang GL,Xu H,et al.Determination of taurine in fish sauce by program sampling-precolumn derivatization-ultra performance liquid chromatography[J].China Condim,2017,42(5):138-140.
[8]黄丼丹,邢亚东,王玲玲,等.2,4-二硝基氟苯柱前衍生化HPLC法测定保健食品中牛磺酸含量[J].药物分析杂志,2016,36(1):171-175.Huang LD,Xing YD,Wang LL,et al.Determination of taurine in health food by RP-HPLC with pre-column derivatization[J].Chin J Pharm Anal,2016,36(1):171-175.
[9]邹晓莉,黎源倩,曾红燕,等.脉冲安培检测-高效阴离子色谱对药品和保健食品中牛磺酸的快速测定[J].分析测试学报,2009,28(4):470.Zou XL,Li YQ,Zeng HY,et al.Quick determination of turine in drug and health food by pulse amper detection-high performance anion exchange chromatography[J].J Instrum Anal,2009,28(4):470.
[10]康明琴,陈明岩,王岸英,等.UPLC-ELSD法测定12种保健食品中的牛磺酸[J].药物分析杂志,2017,37(11):1957-1961.Kang MQ,Chen MY,Wang AY,et al.Determination of taurine in health food by ultra-performance liquid chromatography with evaporative light scattering detector[J].Chin J Pharm Anal,2017,37(11):1957-1961.
[11]陈晓峰,周围,解迎双.超高效液相色谱串联质谱法直接测定婴幼儿乳粉中牛磺酸[J].食品科学,2015,36(24):280.Li XF,Zhou W,Xie YS.Direct determination of taurine in infant milk powder by ultra performance liquid chromatography tandem mass spectrometry[J].Food Sci,2015,36(24):280.
[12]薛勇,薛丰,孙成均.功能食品与饮品中牛磺酸的柱前衍生毛细管电泳法快速测定[J].分析测试学报,2010,29(4):386-389.Xue Y,Xue F,Sun CJ.Rapid determination of taurine in functional food and beverage by high performance capillary electrophoresis with pre-column derivatization[J].J Instrum Anal,2010,29(4):386-389.
[13]王洪健,周兴起,冯志强,等.氨基酸自动分析仪测定食品中牛磺酸的方法建立[J].现代食品科技,2012,28(3):348.Wang HJ,Zhou XQ,Feng ZQ,et al.Dtermination of turine in food products by amino acid autoanalyzer[J].Mod Food Sci Technol,2012,28(3):348.
[14]黄岛平,陈秋虹,劳燕文.柱前衍生高效液相色谱法测定红牛维生素功能饮料中牛磺酸含量[J].广西科学院学报,2006,22(S):419-421.Zhang RM,Chen QH,Lao YW.Determination of taurine in red bull vitamin energy drink by precolumn derivatization high performance liquid chromatography[J].J Guangxi Acad Sci,2006,22(S):419-421.
[15]柳小英,毛丼沙,鲍敏洁,等.高效液相色谱法测定海洋生物中牛磺酸的含量[J].安徽农业科学,2017,45(31):1-3,11.Liu XY,Mao LS,Bao MJ,et al.Detection of taurine in marine life by high performance liquid chromatography[J].J Anhui Agric Sci,2017,45(31):1-3,11.
[16]Ubuka T,Okada A,Nakamura H.Production of hypotaurine fromL-cysteinesulfinate by rat liver mitochondria[J].Amino Acid,2008,35(1):53-58.
[17]叶思平,陈悦娇,陈海光,等.高效液相色谱测定益智仁中的牛磺酸含量[J].食品科学,2013,34(1):204-206.Ye SP,Chen YJ,Chen HG,et al.Determination of taurine in alpinia oxyphylla by high performance liquid chromatography[J].Food Sci,2013,34(1):204-206.
[18]GB 5009.169-2016食品安全国家标准食品中牛磺酸的测定[S].GB 5009.169-2016 National food safety standard-Determination of taurine in foods[S].
[2]陈秋虹,莫建光,黄艳.天然牛磺酸的提取与应用[J].氨基酸和生物资源,2011,33(2):43-45.Chen QH,Mo JG,Huang Y.Extraction and application of natural taurine[J].Amino Acids Biotic Res,2011,33(2):43-45.
[3]刘亚风,袁萍,祝伟霞,等.牛磺酸检测技术研究进展[J].中国卫生检验杂志,2010,20(7):1831-1833.Liu YF,Yuan P,Zhu WX,et al.Research progress of taurine detection technology[J].Chin J Health Lab Technol,2010,20(7):1831-1833.
[4]卢忠,罗一帆.中和滴定法测定牛磺酸含量[J].数理医药学杂志,2000,13(4):350.Lu Z,Luo YF.Determination of taurine by neutralization titration[J].JMathemat Med,2000,13(4):350.
[5]杨祖英,张平伟.薄层色谱法测定食品中牛磺酸[J].卫生研究,1996,25(4):248-250.Yang ZY,Zhang PW.Determination of taurine in food by thin layer chromatography[J].J Hyg Res,1996,25(4):248-250.
[6]王海澍.高效液相色谱法测定牡蛎中牛磺酸含量[J].食品安全导刊,2017,(3):90.Wang HS.Determination of taurine in oyster by HPLC[J].China Food Saf Magaz,2017,(3):90.
[7]孟冰冰,杨桂玲,徐惠,等.程序进样-柱前衍生-超高效液相色谱法测定鱼露酱油中的牛磺酸含量[J].中国调味品,2017,42(5):138-140.Meng BB,Yang GL,Xu H,et al.Determination of taurine in fish sauce by program sampling-precolumn derivatization-ultra performance liquid chromatography[J].China Condim,2017,42(5):138-140.
[8]黄丼丹,邢亚东,王玲玲,等.2,4-二硝基氟苯柱前衍生化HPLC法测定保健食品中牛磺酸含量[J].药物分析杂志,2016,36(1):171-175.Huang LD,Xing YD,Wang LL,et al.Determination of taurine in health food by RP-HPLC with pre-column derivatization[J].Chin J Pharm Anal,2016,36(1):171-175.
[9]邹晓莉,黎源倩,曾红燕,等.脉冲安培检测-高效阴离子色谱对药品和保健食品中牛磺酸的快速测定[J].分析测试学报,2009,28(4):470.Zou XL,Li YQ,Zeng HY,et al.Quick determination of turine in drug and health food by pulse amper detection-high performance anion exchange chromatography[J].J Instrum Anal,2009,28(4):470.
[10]康明琴,陈明岩,王岸英,等.UPLC-ELSD法测定12种保健食品中的牛磺酸[J].药物分析杂志,2017,37(11):1957-1961.Kang MQ,Chen MY,Wang AY,et al.Determination of taurine in health food by ultra-performance liquid chromatography with evaporative light scattering detector[J].Chin J Pharm Anal,2017,37(11):1957-1961.
[11]陈晓峰,周围,解迎双.超高效液相色谱串联质谱法直接测定婴幼儿乳粉中牛磺酸[J].食品科学,2015,36(24):280.Li XF,Zhou W,Xie YS.Direct determination of taurine in infant milk powder by ultra performance liquid chromatography tandem mass spectrometry[J].Food Sci,2015,36(24):280.
[12]薛勇,薛丰,孙成均.功能食品与饮品中牛磺酸的柱前衍生毛细管电泳法快速测定[J].分析测试学报,2010,29(4):386-389.Xue Y,Xue F,Sun CJ.Rapid determination of taurine in functional food and beverage by high performance capillary electrophoresis with pre-column derivatization[J].J Instrum Anal,2010,29(4):386-389.
[13]王洪健,周兴起,冯志强,等.氨基酸自动分析仪测定食品中牛磺酸的方法建立[J].现代食品科技,2012,28(3):348.Wang HJ,Zhou XQ,Feng ZQ,et al.Dtermination of turine in food products by amino acid autoanalyzer[J].Mod Food Sci Technol,2012,28(3):348.
[14]黄岛平,陈秋虹,劳燕文.柱前衍生高效液相色谱法测定红牛维生素功能饮料中牛磺酸含量[J].广西科学院学报,2006,22(S):419-421.Zhang RM,Chen QH,Lao YW.Determination of taurine in red bull vitamin energy drink by precolumn derivatization high performance liquid chromatography[J].J Guangxi Acad Sci,2006,22(S):419-421.
[15]柳小英,毛丼沙,鲍敏洁,等.高效液相色谱法测定海洋生物中牛磺酸的含量[J].安徽农业科学,2017,45(31):1-3,11.Liu XY,Mao LS,Bao MJ,et al.Detection of taurine in marine life by high performance liquid chromatography[J].J Anhui Agric Sci,2017,45(31):1-3,11.
[16]Ubuka T,Okada A,Nakamura H.Production of hypotaurine fromL-cysteinesulfinate by rat liver mitochondria[J].Amino Acid,2008,35(1):53-58.
[17]叶思平,陈悦娇,陈海光,等.高效液相色谱测定益智仁中的牛磺酸含量[J].食品科学,2013,34(1):204-206.Ye SP,Chen YJ,Chen HG,et al.Determination of taurine in alpinia oxyphylla by high performance liquid chromatography[J].Food Sci,2013,34(1):204-206.
[18]GB 5009.169-2016食品安全国家标准食品中牛磺酸的测定[S].GB 5009.169-2016 National food safety standard-Determination of taurine in foods[S].