十二烷基二甲基甜菜碱协助泡沫分离虎杖中白藜芦醇
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摘要
为了开发简单高效的从虎杖中提取白藜芦醇的方法,促进白藜芦醇的产业化应用,本文采用水提和泡沫分离相结合的方法,在泡沫分离过程中选用十二烷基二甲基甜菜碱作为捕获剂,泡沫分离虎杖浸提液中白藜芦醇。研究了椭球型泡沫分离塔、pH、十二烷基二甲基甜菜碱浓度、气体体积流量对泡沫分离白藜芦醇分离效率的影响,并对分离前后白藜芦醇的抗氧化性进行了研究。结果表明,采用椭球型泡沫分离塔,在p H为3、十二烷基二甲基甜菜碱浓度1.50g/L以及气体体积流量20m L/min的条件下,白藜芦醇富集比和回收率分别为8.43和81.73%,消泡液中白藜芦醇浓度为0.085g/L,而且泡沫分离过程中能有效保持白藜芦醇的生物活性。
To promote the industrialization of resveratrol application,the dodecyl dimethyl betaine(BS-12)was selected as collector to recover resveratrol from Giant knotweed leaching solution by foam separation.This paper studied the effects of ellipsoid type foam separation tower,p H,dodecyl dimethyl betaine concentration and volumetric air flow rate on resveratrol separation efficiency.The results showed that the suitable operation conditions were leaching solution p H 3,the odecyl dimethyl betaine concentration 1.50 g/L,and volumetric air flow rate 20 m L/min.Under those operation conditions,the enrichment ratio and the recovery percentage of resveratrol were 8.43 and 81.73%,respectively.Then,the concentration of resveratrol in the foamate was 0.085 g/L.Furthermore,the biological activity of resveratrol could be kept during the process of foam separation.The present studies developed a green technology for effectively separating resveratrol from Giant knotweed leaching solution and then promoting the development and utilization of resveratrol.
To promote the industrialization of resveratrol application,the dodecyl dimethyl betaine(BS-12)was selected as collector to recover resveratrol from Giant knotweed leaching solution by foam separation.This paper studied the effects of ellipsoid type foam separation tower,p H,dodecyl dimethyl betaine concentration and volumetric air flow rate on resveratrol separation efficiency.The results showed that the suitable operation conditions were leaching solution p H 3,the odecyl dimethyl betaine concentration 1.50 g/L,and volumetric air flow rate 20 m L/min.Under those operation conditions,the enrichment ratio and the recovery percentage of resveratrol were 8.43 and 81.73%,respectively.Then,the concentration of resveratrol in the foamate was 0.085 g/L.Furthermore,the biological activity of resveratrol could be kept during the process of foam separation.The present studies developed a green technology for effectively separating resveratrol from Giant knotweed leaching solution and then promoting the development and utilization of resveratrol.
引文
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[9]李燕,刘军海.大孔树脂对虎杖白藜芦醇的静态吸附动力学研究[J].食品与机械,2011(5):82-86.LI Y,LIU J H.Study on the static adsorption kinetics of resveratrol from Polygonum cuspidatum sieb.et zucc by macroporous resin[J].Food and Machinery,2011(5):82-86.
[10]王莹,李梦耀,马岚,等.虎杖中白藜芦醇的提取与纯化[J].应用化工,2014,43(1):128-131.WANG Y,LI M Y,MA L,et al.Extraction and purification of resveratrol from Polygonum cuspidatum[J].Applied Chemical Industry,2014,43(1):128-131.
[11]常志东,刘会洲,陈家镛.泡沫分离法的应用与发展[J].化工进展,1999,18(5):18-21.CHANG Z D,LIU H Z,CHEN J Y.The application and development of foam separation[J].Chemical Industry and Engineering Progress,1999,18(5):18-21.
[12]谭相伟,吴兆亮,贾永生,等.泡沫分离技术在蛋白质多元体系分离中的应用[J].化工进展,2005,24(5):510-513.TAN X W,WU Z L,JIA Y S,et al.Application of separation in multicomponent mixture of proteins[J].Chemical Industry and Engineering Progress,2005,24(5):510-513.
[13]孙瑞娉,殷昊,卢珂,等.两级泡沫分离废水中大豆蛋白的工艺[J].农业工程学报,2010(11):374-378.SUN R P,YIN H,LU K,et al.Technology of soy protein separation from wastewater by two-stage foam fractionation[J].Transactions of the Chinese Society of Agricultural Engineering,2010(11):374-378.
[14]BURGHOFF B.Foam fractionation applications[J].Journal of Biotechnology,2012,161(2):126-137.
[15]丁琳琳,王延吉,吴兆亮,等.基于甘草配伍黄芩同时泡沫分离甘草酸和黄芩苷[J].化工学报,2017,68(1):170-177.DING L L,WANG Y J,WU Z L,et al.Simultaneous separation of glycyrrhizic acid and baicalin by mixing Glycyrrhiza with Scutellaria using foam fractionation[J].CIESC Journal,2017,68(1):170-177.
[16]杨向平,刘元东,秦海良,等.大豆蛋白废水中乳清蛋白的泡沫分离实验[J].化工进展,2008,27(1):92-97.YANG X P,LIU Y D,QIN H L,et al.Experimental research on recovery of lactoalbumin from soy protein wastewater by foam fractionation[J].Chemical Industry and Engineering Progress,2008,27(1):92-97.
[17]JIANG J X,WU Z L,LIU W,et al.Separation of soybean saponins from soybean meal by a technology of foam fractionation and resin adsorption[J].Preparative Biochemistry and Biotechnology,2016,46(4):346-353.
[18]LIU W,WU Z L,WANG Y J,et al.Rhamnolipid assisted recovery of lycopene from the tomato-based processing wastewater using foam fractionation[J].Journal of Food Engineering,2015,164:63-69.
[19]CHEN Z G,DING W F,REN F L,et al.A simple and sensitive assay of nucleic acids based on the enhanced resonance light scattering of zwitterionics[J].Analytica Chimica Acta,2005,550(1):204-209.
[20]ZHANG Z,WU Z L,LIU G M.Interfacial adsorption of methyl orange in liquid phase of foam fractionation using dodecyl dimethyl betaine as the collector[J].Journal of Industrial and Engineering Chemistry,2015,28:184-189.
[21]牛鹏飞,仇农学,杜寅.苹果渣中不同极性多酚的分离及体外抗氧化活性研究[J].农业工程学报,2008,24(3):238-242.NIU P F,QIU N X,DU Y.Separation of polyphenols from apple pomace based on different polarities and their antioxidative activities in vitro[J].Transactions of the Chinese Society of Agricultural Engineering,2008,24(3):238-242.
[22]程雅芳,李全文,续颖,等.花生茎中白藜芦醇的提取及体外抗氧化性的研究[J].食品工业科技,2011,32(7):144-146.CHENG Y F,LI Q W,XU Y,et al.Study on extraction and antioxidant ability of resveratrol from peanut stem[J].Science and Technology of Food Industry,2011,32(7):144-146.
[23]LI J,WU Z L,LI R.Technology of streptomycin sulfate separation by two-stage foam separation[J].Biotechnology Progress,2012,28(3):733-739.
[24]LAMBERT W D,DU L P,MA Y F,et al.The effect of p H on the foam fractionation ofβ-glucosidase and cellulose[J].Bio-resource Technology,2003,87(3):247-253.
[25]张雅杰,佟连生,高敬茹,等.两种芦丁提取方法p H值的比较研究[J].黑龙江医药科学,2008,31(3):44-44.ZHANG Y J,TONG L S,GAO J R,et al.Study on two kinds of method to extract rutin comparing p H[J].Heilongjiang Medicine and Pharmacy,2008,31(3):44-44
[26]LI H Z,WU Z L,LIU W,et al.Recovery of yam mucilage from the yam starch processing wastewater by using a novel foam fractionation column[J].Separation and Purification Technology,2016,171:26-33.
[2]韩雪莲.白藜芦醇及其衍生物和类似物抗肿瘤研究进展[J].化工进展,2014,33(6):1526-1532.HAN X L.Progress in anti-tumor research on resveratrol and its derivatives and analogues[J].Chemical Industry and Engineering Progress,2014,33(6):1526-1532.
[3]李先宽,李赫宇,李帅,等.白藜芦醇研究进展[J].中草药,2016,47(14):2568-2578.LI X K,LI H Y,LI S,et al.Advances in study on resveratrol[J].Chinese Traditional and Herbal Drugs,2016,47(14):2568-2578.
[4]左惠荣,司瑞,党晶艺,等.白藜芦醇对心肌微血管内皮细胞缺氧/复氧的保护及作用机制[J].中华临床医师杂志,2012,6(20):183-187.ZUO H R,SI R,DANG J,et al.Protective effect and its mechanism of resveratrol on hypoxia/reoxygenation-induced apoptosis in cardiac microvascular endothelial cells[J].Chinese Journal of Clinicians(Electronic Edition),2012,6(20):183-187.
[5]张敏,曹庸,于华忠,等.虎杖白藜芦醇提取工艺的初步研究[J].林产化工通讯,2004,38(3):6-9.ZHANG M,CAO Y,YU H Z,et al.Preliminary study on extraction process of resveratrol from polygonum cuspidatum[J].Journal of Chemical Industry of Forest Products,2004,38(3):6-9.
[6]邓梦茹,刘韶,朱周觐.酶法提取虎杖中的白藜芦醇[J].中南药学,2011(9):669-672.DENG M R,LIU S,ZHU Z J.Optimization of resveratrol from Polygonum cuspidatum by enzyme-assisted extraction[J].Central South Pharmacy,2011(9):669-672.
[7]张琳虹.虎杖有效成分的超临界二氧化碳萃取技术研究[J].中国药物与临床,2012,12(3):338-340.ZHANG H L.The technology research of active ingredients from Polygonum cuspidatum by supercritical carbon dioxide[J].Chinese Remedies and Clinics,2012,12(3):338-340.
[8]张初署,禹山林,潘丽娟,等.花生植株白藜芦醇提取工艺优化[J].农业工程学报,2009(s1):148-152.ZHANG C S,YU S L,PAN L J,et al.Technology optimization for extraction of resveratrol from peanut plant[J].Transactions of the Chinese Society of Agricultural Engineering,2009(s1):148-152.
[9]李燕,刘军海.大孔树脂对虎杖白藜芦醇的静态吸附动力学研究[J].食品与机械,2011(5):82-86.LI Y,LIU J H.Study on the static adsorption kinetics of resveratrol from Polygonum cuspidatum sieb.et zucc by macroporous resin[J].Food and Machinery,2011(5):82-86.
[10]王莹,李梦耀,马岚,等.虎杖中白藜芦醇的提取与纯化[J].应用化工,2014,43(1):128-131.WANG Y,LI M Y,MA L,et al.Extraction and purification of resveratrol from Polygonum cuspidatum[J].Applied Chemical Industry,2014,43(1):128-131.
[11]常志东,刘会洲,陈家镛.泡沫分离法的应用与发展[J].化工进展,1999,18(5):18-21.CHANG Z D,LIU H Z,CHEN J Y.The application and development of foam separation[J].Chemical Industry and Engineering Progress,1999,18(5):18-21.
[12]谭相伟,吴兆亮,贾永生,等.泡沫分离技术在蛋白质多元体系分离中的应用[J].化工进展,2005,24(5):510-513.TAN X W,WU Z L,JIA Y S,et al.Application of separation in multicomponent mixture of proteins[J].Chemical Industry and Engineering Progress,2005,24(5):510-513.
[13]孙瑞娉,殷昊,卢珂,等.两级泡沫分离废水中大豆蛋白的工艺[J].农业工程学报,2010(11):374-378.SUN R P,YIN H,LU K,et al.Technology of soy protein separation from wastewater by two-stage foam fractionation[J].Transactions of the Chinese Society of Agricultural Engineering,2010(11):374-378.
[14]BURGHOFF B.Foam fractionation applications[J].Journal of Biotechnology,2012,161(2):126-137.
[15]丁琳琳,王延吉,吴兆亮,等.基于甘草配伍黄芩同时泡沫分离甘草酸和黄芩苷[J].化工学报,2017,68(1):170-177.DING L L,WANG Y J,WU Z L,et al.Simultaneous separation of glycyrrhizic acid and baicalin by mixing Glycyrrhiza with Scutellaria using foam fractionation[J].CIESC Journal,2017,68(1):170-177.
[16]杨向平,刘元东,秦海良,等.大豆蛋白废水中乳清蛋白的泡沫分离实验[J].化工进展,2008,27(1):92-97.YANG X P,LIU Y D,QIN H L,et al.Experimental research on recovery of lactoalbumin from soy protein wastewater by foam fractionation[J].Chemical Industry and Engineering Progress,2008,27(1):92-97.
[17]JIANG J X,WU Z L,LIU W,et al.Separation of soybean saponins from soybean meal by a technology of foam fractionation and resin adsorption[J].Preparative Biochemistry and Biotechnology,2016,46(4):346-353.
[18]LIU W,WU Z L,WANG Y J,et al.Rhamnolipid assisted recovery of lycopene from the tomato-based processing wastewater using foam fractionation[J].Journal of Food Engineering,2015,164:63-69.
[19]CHEN Z G,DING W F,REN F L,et al.A simple and sensitive assay of nucleic acids based on the enhanced resonance light scattering of zwitterionics[J].Analytica Chimica Acta,2005,550(1):204-209.
[20]ZHANG Z,WU Z L,LIU G M.Interfacial adsorption of methyl orange in liquid phase of foam fractionation using dodecyl dimethyl betaine as the collector[J].Journal of Industrial and Engineering Chemistry,2015,28:184-189.
[21]牛鹏飞,仇农学,杜寅.苹果渣中不同极性多酚的分离及体外抗氧化活性研究[J].农业工程学报,2008,24(3):238-242.NIU P F,QIU N X,DU Y.Separation of polyphenols from apple pomace based on different polarities and their antioxidative activities in vitro[J].Transactions of the Chinese Society of Agricultural Engineering,2008,24(3):238-242.
[22]程雅芳,李全文,续颖,等.花生茎中白藜芦醇的提取及体外抗氧化性的研究[J].食品工业科技,2011,32(7):144-146.CHENG Y F,LI Q W,XU Y,et al.Study on extraction and antioxidant ability of resveratrol from peanut stem[J].Science and Technology of Food Industry,2011,32(7):144-146.
[23]LI J,WU Z L,LI R.Technology of streptomycin sulfate separation by two-stage foam separation[J].Biotechnology Progress,2012,28(3):733-739.
[24]LAMBERT W D,DU L P,MA Y F,et al.The effect of p H on the foam fractionation ofβ-glucosidase and cellulose[J].Bio-resource Technology,2003,87(3):247-253.
[25]张雅杰,佟连生,高敬茹,等.两种芦丁提取方法p H值的比较研究[J].黑龙江医药科学,2008,31(3):44-44.ZHANG Y J,TONG L S,GAO J R,et al.Study on two kinds of method to extract rutin comparing p H[J].Heilongjiang Medicine and Pharmacy,2008,31(3):44-44
[26]LI H Z,WU Z L,LIU W,et al.Recovery of yam mucilage from the yam starch processing wastewater by using a novel foam fractionation column[J].Separation and Purification Technology,2016,171:26-33.