新胃乃安片中4种异黄酮成分的含量测定及一测多评法的建立
|
摘要
目的:建立同时测定新胃乃安片中毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素等4种异黄酮成分的含量测定方法。方法:采用HPLC外标法测定上述4种异黄酮成分的含量,以毛蕊异黄酮苷为内参物,建立毛蕊异黄酮苷与芒柄花苷、毛蕊异黄酮、芒柄花素的相对校正因子,通过相对校正因子计算出芒柄花苷、毛蕊异黄酮、芒柄花素的量,计算结果与外标法实测值进行比较。结果:建立了一测多评同时测定新胃乃安片中4种异黄酮成分的含量测定方法,该方法与外标法测定结果没有明显差异。结论:该研究建立的一测多评法简单方便,可同时测定新胃乃安片中4种异黄酮类成分含量,为建立完善的新胃乃安片质量标准提供参考依据。
Objective:To establish a method for simultaneously determination of four isoflavones(calycosin-7-O-β-D-glucopyranoside,ononin,calycosin,formononetin)in Xin Wei Nai An Tablet.Methods:The external standard of HPLC method was used to simultaneously determine the contents of four isoflavones in Xin Wei Nai An Tablet.Calycosin-7-O-β-D-glucopyranoside was taken as internal standard substance to calculate the relative correction factors(RCFs)for calculated the content of ononin,calycosin,formononetin.The obtainedresults were then compared with the external standard method results.Results:The quantitative analysis method of multi-component with a single-maker(QAMS)was established to simultaneously determine four isoflavones in Xin Wei Nai An Tablet.The results calculated with QAMS were consistent with the results by the external standard method.Conclusion:QAMS method is simple,feasible,and reproducible,which could provide a reference for further research on completing the quality control of Xin Wei Nai An Tablet.
Objective:To establish a method for simultaneously determination of four isoflavones(calycosin-7-O-β-D-glucopyranoside,ononin,calycosin,formononetin)in Xin Wei Nai An Tablet.Methods:The external standard of HPLC method was used to simultaneously determine the contents of four isoflavones in Xin Wei Nai An Tablet.Calycosin-7-O-β-D-glucopyranoside was taken as internal standard substance to calculate the relative correction factors(RCFs)for calculated the content of ononin,calycosin,formononetin.The obtainedresults were then compared with the external standard method results.Results:The quantitative analysis method of multi-component with a single-maker(QAMS)was established to simultaneously determine four isoflavones in Xin Wei Nai An Tablet.The results calculated with QAMS were consistent with the results by the external standard method.Conclusion:QAMS method is simple,feasible,and reproducible,which could provide a reference for further research on completing the quality control of Xin Wei Nai An Tablet.
引文
[1] 林传权.胃乃安新制剂提取分离工艺研究及抗胃黏膜损伤机制初探[D].广州:广州中医药大学,2011.
[2] 陈娟,刘佳,秦泽慧,等.新胃乃安片对胃黏膜损伤的作用[J].中药药理与临床,2013,29(4):131-134.
[3] 梁小银,林文华,严萍,等.新胃乃安片的质量标准研究[J].中成药,2014,36(5):990-994.
[4] 孙政华,邵晶,郭玫.黄芪化学成分及药理作用研究进展[J].中医临床研究,2015,7(25):22-25.
[5] 国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015.
[6] 于玲,王知斌,王秋红,等.黄芪中黄酮类化合物药理作用研究进展[J].中医药信息,2018,35(2):104-108.
[7] 蔡海霞,陈君,李萍.一测多评法测定黄芪中4种异黄酮的含量[J].中国中药杂志,2010,35(20):2712-2717.
[8] 王智民,钱忠直,张启伟,等.一测多评法建立的技术指南[J].中国中药杂志,2011,36(6):657-658.
[9] 唐爽,包永睿,孟宪生,等.基于一测多评法评价天麻活性成分的质量控制方法[J].中国现代中药,2016,18(9):1135-1152.
[10] 吴文杰,邓阳,谭桂林,等.一测多评法测定葛根药材中5种异黄酮类成分[J].中草药,2017,48(4):777-781.
[11] 陈杨,徐光临,徐德生,等.一测多评法在中药质量控制中的应用及关键问题[J].环球中医药,2017,10(5):635-640.
[12] ZHANG W,JIANG S,QIAN D,et al.Analysis of interaction property of calycosin-7-O-β-D-glucoside with human gut microbiota[J].J Chromatogr B,2014,963:16-23.
[2] 陈娟,刘佳,秦泽慧,等.新胃乃安片对胃黏膜损伤的作用[J].中药药理与临床,2013,29(4):131-134.
[3] 梁小银,林文华,严萍,等.新胃乃安片的质量标准研究[J].中成药,2014,36(5):990-994.
[4] 孙政华,邵晶,郭玫.黄芪化学成分及药理作用研究进展[J].中医临床研究,2015,7(25):22-25.
[5] 国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015.
[6] 于玲,王知斌,王秋红,等.黄芪中黄酮类化合物药理作用研究进展[J].中医药信息,2018,35(2):104-108.
[7] 蔡海霞,陈君,李萍.一测多评法测定黄芪中4种异黄酮的含量[J].中国中药杂志,2010,35(20):2712-2717.
[8] 王智民,钱忠直,张启伟,等.一测多评法建立的技术指南[J].中国中药杂志,2011,36(6):657-658.
[9] 唐爽,包永睿,孟宪生,等.基于一测多评法评价天麻活性成分的质量控制方法[J].中国现代中药,2016,18(9):1135-1152.
[10] 吴文杰,邓阳,谭桂林,等.一测多评法测定葛根药材中5种异黄酮类成分[J].中草药,2017,48(4):777-781.
[11] 陈杨,徐光临,徐德生,等.一测多评法在中药质量控制中的应用及关键问题[J].环球中医药,2017,10(5):635-640.
[12] ZHANG W,JIANG S,QIAN D,et al.Analysis of interaction property of calycosin-7-O-β-D-glucoside with human gut microbiota[J].J Chromatogr B,2014,963:16-23.