虹鳟中草药免疫增强剂体外快速筛选研究
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摘要
实验以虹鳟为研究对象,选取17种中草药开展中草药免疫增强剂的体外快速筛选研究。采用Percoll密度梯度离心方法,对虹鳟外周血白细胞进行分离纯化实验。结果表明:白头翁、桂桔、白芨、大黄、贯众、红景天6种中草药的SPO显著高于对照组(P<0.05),且NBTN显著提高[NBTB(%)>15%];白芨、厚朴、麻黄、丹参、大黄、马齿苋、泽泻、黄柏、苍耳子、贯众、红景天组的吞噬活性(PA)值显著高于对照组(P<0.05),蒲公英组的吞噬指数(PI)值显著高于对照组(P<0.05)。综合上述评价指标:白芨、大黄、贯众、红景天可作为虹鳟免疫增强剂的备选中草药。
This research was conducted to deter-mine 17 Chinese herbal medicines were selectedfor rapid screening of immunostimulants of O.mykiss in vitro. Percoll density gradient centrifu-gation methods was used to isolate and purifywhite blood cells from peripheral blood of O.mykiss. The results showed that SPO-values ofAnemone chinensis, Cassia twig, Rhizoma bletil-lae, Radixet rhizomarhei, Cyrtomium rhizomeand Rhodiola rosea were significantly highest compared with control(P<0.05), and NBTBvalues were significantly improved[NBTB(%)>15%]. That PAvalues of Rhizoma bletillae, Cyrtomium rhizome, Rhodiola rosea, Cortex phellodendri, Fructus xanthii,Radix et rhizoma rhei, Port ulaca oleracea L, Rhizoma alismatis, Herba ephedrae, Radix salviae miltior-rhizae and Magnolia bark were significantly highest compared with control group(P<0.05), the PI val-ues of Taraxacum mongolicum were significantly highest compared with control group(P<0.05). Over-all, four kinds of Chinese herbal medicines such as white Radix Paeoniae Alba, Radix et rhizoma rhei,Cyrtomium rhizome, and Rhodiola rosea can be used as immunostimulants for O. mykiss.
This research was conducted to deter-mine 17 Chinese herbal medicines were selectedfor rapid screening of immunostimulants of O.mykiss in vitro. Percoll density gradient centrifu-gation methods was used to isolate and purifywhite blood cells from peripheral blood of O.mykiss. The results showed that SPO-values ofAnemone chinensis, Cassia twig, Rhizoma bletil-lae, Radixet rhizomarhei, Cyrtomium rhizomeand Rhodiola rosea were significantly highest compared with control(P<0.05), and NBTBvalues were significantly improved[NBTB(%)>15%]. That PAvalues of Rhizoma bletillae, Cyrtomium rhizome, Rhodiola rosea, Cortex phellodendri, Fructus xanthii,Radix et rhizoma rhei, Port ulaca oleracea L, Rhizoma alismatis, Herba ephedrae, Radix salviae miltior-rhizae and Magnolia bark were significantly highest compared with control group(P<0.05), the PI val-ues of Taraxacum mongolicum were significantly highest compared with control group(P<0.05). Over-all, four kinds of Chinese herbal medicines such as white Radix Paeoniae Alba, Radix et rhizoma rhei,Cyrtomium rhizome, and Rhodiola rosea can be used as immunostimulants for O. mykiss.
引文
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[2]农业部渔业渔政管理局编制.中国渔业统计年鉴2018[M].北京:中国农业出版社,2018.
[3]谢仲权,牛淑琦.天然植物饲料添加剂生产技术与质量标准[M].北京:中国农业科学技术出版社,2005:23-28.
[4]邢跃楠,赵文.中草药在水产养殖中应用的研究进展[J].中国水产,2007,377(4):64-66.
[5]胡国宏,徐有国,金香琴,等.中草药对虹鳟稚鱼免疫力的影响[J].黑龙江水产, 2018(5):30-32.
[6]孙裔雷.复方中草药对虹鳟抗氧化能力的影响[J].大连海洋大学学报,2015,30(2):170-174.
[7]陈孝煊,吴志新,殷居易,等.大黄、穿心莲、板蓝根和金银花对异育银鲫免疫机能的影响[J].中国水产科学,2003,10(1):36-40.
[8]张意敏.复方中草药和果寡糖对罗非鱼免疫性能的影响[D].上海:上海海洋大学,2013.
[9]陈伟,马小琴,范文玺.红景天主要成分对小鼠免疫细胞的促增殖转化作用[J].中国现代应用药学,2016,33(1):38-42.
[10]邹芝英,祝璟琳,杨弘,等.饲料中添加红景天苷对尼罗罗非鱼血液学指标的影响[J].淡水渔业,2012,42(6):82-85.
[11]王庆奎,陈成勋,郭永军,等. 7种中草药对斑点叉尾鮰生理生化指标的影响[J].安徽农业科学,2011,39(15):9305-9308.
[12]刘红柏,宿斌,王荻.中草药方剂对哲罗鱼抗氧化能力的影响[J].东北农业大学学报,2012(9):127-134.