电厂冷却水培养核诱变微藻固定15%CO_2
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摘要
经~(60)Co-γ射线核辐射诱变、高盐度定向筛选和高浓度CO_2梯度驯化,获得了可耐受15%CO_2(体积分数)和海水盐度的核诱变小球藻固碳藻株。利用实际电厂循环冷却海水培养核诱变藻株固定模拟燃煤烟气15%CO_2,结果表明:核诱变藻株在加入了氮、磷营养盐的冷却海水中比生长速率可达1. 42/d,生物生产力可达0. 72 g/(L·d),与f/2海水培养基培养的核诱变藻株相比,分别提高了10%和28%。微藻固碳效率受盐度影响,核诱变藻株在电厂循环冷却海水中的固碳速率可达0. 68 g/(L·d),为同期f/2海水培养基条件下固碳速率的1. 9倍。PCA分析表明,15%CO_2条件下循环冷却海水各组分对微藻生长贡献基本相当。利用循环冷却海水培养微藻可有效固定煤电行业烟气中15%CO_2,为燃煤电厂废水废气资源化综合利用提供了新思路。
Nuclear mutated chlorella that can tolerate 15% CO_2( by volume) and seawater with certain salinity,as the algal strain for carbon fixation,was obtained by irradiation mutation of60 Co-γ ray,high-salinity directed screening and highconcentration CO_2 gradient domestication. The nuclear mutated algal strain was cultured with actual circulating cooling seawater from power plants,in order to fix CO_2 in simulated coal-fired flue gas with a volume pecentage of 15%. The results showed that the specific growth rate and biological productivity of the mutant were 1. 42/d and 0. 72 g/( L·d) respectively in the cooling seawater with nitrogen and phosphorus nutrients,which were 10% and 28% respectively higher than those in the f/2 seawater culture medium. Affected by the salinity,the CO_2 fixation rate of the mutant in the circulating cooling seawater from power plants reached 0. 68 g/( L·d),1. 9 times higher than that in the f/2 seawater culture medium. PCA showed that all component of circulating cooling seawater made equivalent contribution to the growth of microalgae under the condition of 15% CO_2. The cultivation of microalgae with the circulation cooling seawater for 15% CO_2 fixation can provide a new way for comprehensive recycling utilization of waste water and gas from coal-fired power plants.
Nuclear mutated chlorella that can tolerate 15% CO_2( by volume) and seawater with certain salinity,as the algal strain for carbon fixation,was obtained by irradiation mutation of60 Co-γ ray,high-salinity directed screening and highconcentration CO_2 gradient domestication. The nuclear mutated algal strain was cultured with actual circulating cooling seawater from power plants,in order to fix CO_2 in simulated coal-fired flue gas with a volume pecentage of 15%. The results showed that the specific growth rate and biological productivity of the mutant were 1. 42/d and 0. 72 g/( L·d) respectively in the cooling seawater with nitrogen and phosphorus nutrients,which were 10% and 28% respectively higher than those in the f/2 seawater culture medium. Affected by the salinity,the CO_2 fixation rate of the mutant in the circulating cooling seawater from power plants reached 0. 68 g/( L·d),1. 9 times higher than that in the f/2 seawater culture medium. PCA showed that all component of circulating cooling seawater made equivalent contribution to the growth of microalgae under the condition of 15% CO_2. The cultivation of microalgae with the circulation cooling seawater for 15% CO_2 fixation can provide a new way for comprehensive recycling utilization of waste water and gas from coal-fired power plants.
引文
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[15] Cheng J, Li K, Yang Z, et al. Gradient domestication of Haematococcus pluvialis mutant with 15%CO2to promote biomass growth and astaxanthin yield[J]. Bioresource Technology,2016,216:340-344.
[2] Geetanjali Y,Ankush K,Kumar Dash S,et al. Performance evaluation of a green process for microalgal CO2sequestration in closed photobioreactor using flue gas generated in-situ[J].Bioresource Technology,2015,191:399-406.
[3] Li F F,Yang Z H,Zeng R,et al. Microalgae capture of CO2from actual flue gas discharged from a combustion chamber[J].Industrial&Engineering Chemistry Research,2011,50(10):6496-6502.
[4]朱顺妮,王亚杰,黄伟,等.缺氮培养对小球藻碳水化合物和油脂积累的影响[J].太阳能学报,2016(8):2118-2122.
[5] Cheah W Y,Show P L,Chang J,et al. Biosequestration of atmospheric CO2and flue gas-containing CO2by microalgae[J].Bioresource Technology,2015,184:190-201.
[6] Cheng J,Huang Y,Feng J,et al. Mutate Chlorella sp by nuclear irradiation to fix high concentrations of CO2[J]. Bioresource Technology,2013,136:496-501.
[7] Cheng J,Li K,Yang Z,et al. Enhancing the growth rate and astaxanthin yield of Haematococcus pluvialis by nuclear irradiation and high concentration of carbon dioxide stress[J]. Bioresource Technology,2016,204:49-54.
[8] Cheng R,Feng J, Zhang B,et al. Transcriptome and gene expression analysis of an oleaginous diatom under different salinity conditions[J]. Bioenergy Research,2014,7(1):192-205.
[9] Luangpipat T,Chisti Y. Biomass and oil production by Chlorella vulgaris and four other microalgae:effects of salinity and other factors[J]. Journal of Biotechnology,2017,257:47-57.
[10] Agarwal R,Rane S S,Sainis J K. Effects of60Co gamma radiation on thylakoid membrane functions in Anacystis nidulans[J]. Journal of Photochemistry And Photobiology B-Biology,2008,91(1):9-19.
[11] Kovacs E,Keresztes A. Effect of gamma and UV-B/C radiation on plant cells[J]. Micron,2002,33(2):199-210.
[12]包杰,田相利,董双林,等.温度、盐度和光照强度对鼠尾藻氮、磷吸收的影响[J].中国水产科学,2008,15(2):293-300.
[13]郭瑾,杨维东,刘洁生,等.温度、盐度和光照对球形棕囊藻生长和产毒的影响研究[J].环境科学学报,2007,27(8):1341-1346.
[14]潘晓华,石庆华,郭进耀,等.无机磷对植物叶片光合作用的影响及其机理的研究进展[J].植物营养与肥料学报,1997,3(3):201-208.
[15] Cheng J, Li K, Yang Z, et al. Gradient domestication of Haematococcus pluvialis mutant with 15%CO2to promote biomass growth and astaxanthin yield[J]. Bioresource Technology,2016,216:340-344.