我国部分地区谷物中脱氧雪腐镰刀菌烯醇含量检测分析
|
摘要
目的描述我国部分地区主要谷物脱氧雪腐镰刀菌烯醇(Deoxynivalenol,DON)污染率及污染水平,探讨谷物DON污染现状。方法采用分层随机抽样收集全国18个省份市售谷物样本7类,应用酶联免疫吸附法检测DON含量。结果 DON检出率由高到低依次为青稞(100.0%),玉米(90.6%),黄豆(85.7%),大米(81.3%),绿豆(76.1%),面粉(74.1%),小米(69.8%);DON平均值由高到低依次为玉米(266.1±326.1)μg/kg,面粉(168.6±219.5)μg/kg,黄豆(161.7±123.4)μg/kg,大米(152.9±199.5)μg/kg,绿豆(136.6±94.9)μg/kg,青稞(120.3±32.8)μg/kg,小米(118.4±93.4)μg/kg。结论我国谷物普遍受到DON污染,其中青稞、玉米、黄豆、大米的检出率均超过80%,从含量上看玉米污染严重,且有个别样本DON含量超出国家限量标准。
Objective To describe the contamination rate and pollution level of deoxynivalenol in the main cereals in some areas of China,and to discuss the current status of DON pollution.Methods A stratified random sampling was used to collect seven types of cereal samples commercially available in 18 provinces nationwide.The DON content was determined by enzyme-linked immunosorbent assay.Results The detection rates of DON from high to low were highland barley(100.0%),corn(90.6%),soybean(85.7%),rice(81.3%),mung bean(76.1%),flour(74.1%)and millet(69.8%);and the average levels of DON from high to low was corn(266.1±326.1)μg/kg,flour(168.6±219.5)μg/kg,soybean(161.7±123.4)μg/kg,rice(152.9±199.5)μg/kg,mung bean(136.6±94.9)μg/kg,highland barley(120.3±32.8)μg/kg,millet(118.4±93.4)μg/kg.Conclusion The grain of China is generally polluted by DON.The detection rate of highland barley,corn,soybean and rice are all over 80%.In terms of DON content,corn was seriously polluted,and the DON content of some samples exceeded the national limit.
Objective To describe the contamination rate and pollution level of deoxynivalenol in the main cereals in some areas of China,and to discuss the current status of DON pollution.Methods A stratified random sampling was used to collect seven types of cereal samples commercially available in 18 provinces nationwide.The DON content was determined by enzyme-linked immunosorbent assay.Results The detection rates of DON from high to low were highland barley(100.0%),corn(90.6%),soybean(85.7%),rice(81.3%),mung bean(76.1%),flour(74.1%)and millet(69.8%);and the average levels of DON from high to low was corn(266.1±326.1)μg/kg,flour(168.6±219.5)μg/kg,soybean(161.7±123.4)μg/kg,rice(152.9±199.5)μg/kg,mung bean(136.6±94.9)μg/kg,highland barley(120.3±32.8)μg/kg,millet(118.4±93.4)μg/kg.Conclusion The grain of China is generally polluted by DON.The detection rate of highland barley,corn,soybean and rice are all over 80%.In terms of DON content,corn was seriously polluted,and the DON content of some samples exceeded the national limit.
引文
[1]李群伟,李金平,侯海峰,等.脱氧雪腐镰刀菌烯醇致幼鼠成年鼠骨及关节软骨损伤差异的实验研究[J].中国预防医学杂志,2011,12(10):813-817.
[2]Gu W,Zhu P,Jiang D,et al.A novel and simple cell-based electrochemical impedance biosensor for evaluating the combined toxicity of DON and ZEN[J].Bio Bioe,2015,70:447-454.
[3]De Ruyck K,De Boevre M,Huybrechts I,et al.Dietary mycotoxins,co-exposure,and carcinogenesis in humans:short review[J].Mutation Research,2015,766:32-41.
[4]陈龙明,李群伟,侯海峰,等.DON与NOC对肝脏致病作用的实验研究[J].中华肿瘤防治杂志,2010,17(21):1711-1713.
[5]陈龙明,李群伟,侯海峰.DON与NOC对胃组织致癌作用的实验研究[J].中华肿瘤防治杂志,2009,16(18):1372-1374.
[6]史建荣,刘馨,仇剑波,等.小麦中镰刀菌毒素脱氧雪腐镰刀菌烯醇污染现状与防控研究进展[J].中国农业科学,2014,47(18):3641-3654.
[7]GB/T2715-2016食品安全国家标准[S].北京:中国标准出版社,2016.
[8]Bindera EM,Tanb LM,Chinb LJ,et al.Worldwide occurrence of mycotoxins in commodities,feeds and feed ingredients[J].An Feed Sci and Technol,2007,137(3/4):265-282.
[9]季海霞,钱英,黄萃茹,等.2015年全国部分地区饲料及原料霉菌毒素分析报告[J].养猪,2016,30(1):21-24.
[10]熊凯华,胡威,汪孟娟,等.安徽河南粮食中脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮的污染调查[J].食品科学,2009,30(20):265-268.
[11]侯海峰,李群伟,李晓梅.温度和湿度对黄绿青霉菌生长和产毒的影响[J].中国地方病防治杂志,2010,25(2):104-106.
[12]刘凤芝,李锋,王永丽.2017年上半年我国部分地区饲料及饲料原料中霉菌毒素的污染状况分析[J].粮食与饲料工业,2017,11(1):46-50.
[13]宋先梅,潘丽梅,高晓岚,等.山西省玉米和小麦中DON、AFB-1和OA的污染状况及相互关系[J].中国食品卫生杂志,1994,3(1):9-11.
[14]宫春波,董峰光,王朝霞.烟台市售谷类及其制品中真菌毒素污染状况调查分析[J].食品研究与开发,2018,39(16):189-194.
[15]邱思奇,徐贞贞,沈红,等.脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮对癌细胞的促增殖和促迁移作用[J].动物营养学报,2018,30(5):1988-1995.
[16]李伟群.真菌毒素与人体健康[M].北京:人民军医出版社,2005:102-113.
[17]吕东晋.青稞及其制品中两种镰刀菌毒素的检测[J].环境与健康杂志,2013,30(4):303-305.
[18]李群伟.大骨节病、克山病地理分布成因研究[J].国外医学(医学地理分册),2010,31(3):139-142.
[19]王丽英,任贝贝,杨立新,等.河北地区谷物及谷物制品中脱氧雪腐镰刀菌烯醇及其衍生物污染水平调查与分析[J].中国食品卫生杂志,2015,27(5):571-575.
[20]熊凯华.粮食中脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮的污染调查及降解毒素微生物的筛选[D].南昌大学,2010.
[2]Gu W,Zhu P,Jiang D,et al.A novel and simple cell-based electrochemical impedance biosensor for evaluating the combined toxicity of DON and ZEN[J].Bio Bioe,2015,70:447-454.
[3]De Ruyck K,De Boevre M,Huybrechts I,et al.Dietary mycotoxins,co-exposure,and carcinogenesis in humans:short review[J].Mutation Research,2015,766:32-41.
[4]陈龙明,李群伟,侯海峰,等.DON与NOC对肝脏致病作用的实验研究[J].中华肿瘤防治杂志,2010,17(21):1711-1713.
[5]陈龙明,李群伟,侯海峰.DON与NOC对胃组织致癌作用的实验研究[J].中华肿瘤防治杂志,2009,16(18):1372-1374.
[6]史建荣,刘馨,仇剑波,等.小麦中镰刀菌毒素脱氧雪腐镰刀菌烯醇污染现状与防控研究进展[J].中国农业科学,2014,47(18):3641-3654.
[7]GB/T2715-2016食品安全国家标准[S].北京:中国标准出版社,2016.
[8]Bindera EM,Tanb LM,Chinb LJ,et al.Worldwide occurrence of mycotoxins in commodities,feeds and feed ingredients[J].An Feed Sci and Technol,2007,137(3/4):265-282.
[9]季海霞,钱英,黄萃茹,等.2015年全国部分地区饲料及原料霉菌毒素分析报告[J].养猪,2016,30(1):21-24.
[10]熊凯华,胡威,汪孟娟,等.安徽河南粮食中脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮的污染调查[J].食品科学,2009,30(20):265-268.
[11]侯海峰,李群伟,李晓梅.温度和湿度对黄绿青霉菌生长和产毒的影响[J].中国地方病防治杂志,2010,25(2):104-106.
[12]刘凤芝,李锋,王永丽.2017年上半年我国部分地区饲料及饲料原料中霉菌毒素的污染状况分析[J].粮食与饲料工业,2017,11(1):46-50.
[13]宋先梅,潘丽梅,高晓岚,等.山西省玉米和小麦中DON、AFB-1和OA的污染状况及相互关系[J].中国食品卫生杂志,1994,3(1):9-11.
[14]宫春波,董峰光,王朝霞.烟台市售谷类及其制品中真菌毒素污染状况调查分析[J].食品研究与开发,2018,39(16):189-194.
[15]邱思奇,徐贞贞,沈红,等.脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮对癌细胞的促增殖和促迁移作用[J].动物营养学报,2018,30(5):1988-1995.
[16]李伟群.真菌毒素与人体健康[M].北京:人民军医出版社,2005:102-113.
[17]吕东晋.青稞及其制品中两种镰刀菌毒素的检测[J].环境与健康杂志,2013,30(4):303-305.
[18]李群伟.大骨节病、克山病地理分布成因研究[J].国外医学(医学地理分册),2010,31(3):139-142.
[19]王丽英,任贝贝,杨立新,等.河北地区谷物及谷物制品中脱氧雪腐镰刀菌烯醇及其衍生物污染水平调查与分析[J].中国食品卫生杂志,2015,27(5):571-575.
[20]熊凯华.粮食中脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮的污染调查及降解毒素微生物的筛选[D].南昌大学,2010.