硝酸镧对大鼠免疫功能影响
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摘要
目的探讨硝酸镧对大鼠免疫的影响。方法将160只SPF级断乳大鼠随机分为两批,每批80只并随机分为对照、低、中、高剂量组,每组20只,雌雄各半,于出生后第24天开始灌胃给予硝酸镧溶液,剂量分别为0、2、20、60 mg/kg BW。出生后第51天对第一批大鼠进行主要脏器重量及脏体比、外周血淋巴细胞分型以及血清细胞因子的检测,出生后第54天对第二批大鼠进行脾T、B淋巴细胞增殖及脾脏抗体生成细胞的测定。结果与对照组比较,雌雄大鼠硝酸镧各剂量组周体质量和终体质量均差异无统计学意义(P>0.05)。雄性大鼠高剂量组肾脏绝对重量和相对重量高于对照组,差异有统计学意义(P<0.05);雌性大鼠低剂量组脑绝对重量和相对重量均高于对照组,差异有统计学意义(P<0.05)。雄性大鼠高剂量组自然杀伤细胞数低于对照组,差异有统计学意义(P<0.05),且存在剂量-反应关系;与对照组比较,雄性大鼠低剂量组CD4/CD8比值升高,雌性大鼠T细胞数增加、B细胞数降低,均差异有统计学意义(P<0.05)。结论本试验条件下硝酸镧对大鼠具有免疫毒性作用,基于免疫毒性测试终点,其免疫毒性作用的基准剂量下限为8.10 mg/kg BW。
Objective To explore the effect of lanthanum nitrate on immunity in rats. Methods One hundred and sixty SPF weaned rats were randomly divided into two batches, 80 rats in each group and randomly divided into control, low, medium and high dose group, with 20 rats(male and female) in each group.On the 24 th day after birth, lanthanum nitrate solution was administered at doses of 0, 2, 20 and 60 mg/kg BW respectively. On the 51 st day after birth, the first batch of rats were tested for main organs, organ-body ratio, peripheral blood lymphocyte type and serum cytokines; splenic T and B lymphocyte proliferation and splenic antibody-producing cells were measured on the 54 th day after birth to the second batch of rats. Results There was no significant difference of body weight among the three dose groups and the control group to the two batch of rats(P>0.05). The absolute and relative weight of kidney and viscera of male rats in middle and high dose groups were significantly higher than those in control group(P<0.05). The brain weight and viscera ratio of female rats in low dose group were higher than those in control group(P<0.05). The number of natural killer cells in the high dose group was significantly lower than that in the control group, with dose-response relationship(P<0.05).The ratio of CD4/CD8 in the low dose group was significantly higher(P<0.05). The number of T cells in the low dose group was significantly higher(P<0.05), however the number of B cells in the low dose group was significantly lower(P<0.05). Based on the immunotoxicity test parameter. Conclusion In this experiment, lanthanum nitrate had immunotoxic effects on rats, based on the immunotoxicity test parameter, the baseline lower limit value of immunotoxicity was 8.10 mg/kg BW.
Objective To explore the effect of lanthanum nitrate on immunity in rats. Methods One hundred and sixty SPF weaned rats were randomly divided into two batches, 80 rats in each group and randomly divided into control, low, medium and high dose group, with 20 rats(male and female) in each group.On the 24 th day after birth, lanthanum nitrate solution was administered at doses of 0, 2, 20 and 60 mg/kg BW respectively. On the 51 st day after birth, the first batch of rats were tested for main organs, organ-body ratio, peripheral blood lymphocyte type and serum cytokines; splenic T and B lymphocyte proliferation and splenic antibody-producing cells were measured on the 54 th day after birth to the second batch of rats. Results There was no significant difference of body weight among the three dose groups and the control group to the two batch of rats(P>0.05). The absolute and relative weight of kidney and viscera of male rats in middle and high dose groups were significantly higher than those in control group(P<0.05). The brain weight and viscera ratio of female rats in low dose group were higher than those in control group(P<0.05). The number of natural killer cells in the high dose group was significantly lower than that in the control group, with dose-response relationship(P<0.05).The ratio of CD4/CD8 in the low dose group was significantly higher(P<0.05). The number of T cells in the low dose group was significantly higher(P<0.05), however the number of B cells in the low dose group was significantly lower(P<0.05). Based on the immunotoxicity test parameter. Conclusion In this experiment, lanthanum nitrate had immunotoxic effects on rats, based on the immunotoxicity test parameter, the baseline lower limit value of immunotoxicity was 8.10 mg/kg BW.
引文
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[9] 李冬梅,黄可欣.长期服用低剂量硝酸镧对大鼠肾脏的影响[J].军医进修学院学报,2008,29(5):418-419.
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[11] 陈兴安,贺秋晨,关腾,等.小剂量柠檬酸稀土对小鼠中性多形核白细胞吞噬功能的影响[J].中国稀土学报,1995,13(1):70-73.
[12] 孙静,秦新英,刘会芳.氯化铒和氯化镝对小鼠免疫细胞作用的体外实验[J].河北大学学报(自然科学版),2012,32(3):281-285.
[13] 崔焕波,艾金霞.SBHL对小鼠免疫功能影响的实验研究[J].中国实验诊断学,2011,15(11):1804-1806.
[2] 汪伟,李冬梅,黄可欣,等.混合稀土染毒对小鼠免疫功能的影响[J].毒理学杂志,2012,26(6):458-459.
[3] 胡爱武,刘小阳,秦宜德.硝酸镧对大鼠甲状腺和胰岛分泌功能的影响[J].毒理学杂志,2009,23(1):56-57.
[4] 马文强,冯杰.稀土元素对动物免疫功能的影响[J].饲料工业,2006,27(4):52-54.
[5] 宋宇,黄可欣,李冬梅,等.硝酸镧对小鼠特异性抗体的形成、淋巴细胞转化和腹腔巨噬细胞吞噬功能的影响[J].吉林大学学报(医学版),2012,38(6):1048-1051.
[6] 刘丹丹,杨辉,张文众,等.孕期硝酸镧暴露对子代大鼠免疫的影响[J].中国食品卫生杂志,2017,29(3):257-263.
[7] 刘丹丹.孕期硝酸镧暴露对仔代大鼠免疫功能的影响[D].太原:山西医科大学,2017.
[8] 宋雁,贾旭东,崔文明,等.不同途径和剂量环磷酰胺建立小鼠免疫抑制模型的对比研究[J].中国食品卫生杂志,2013,25(3):218-225.
[9] 李冬梅,黄可欣.长期服用低剂量硝酸镧对大鼠肾脏的影响[J].军医进修学院学报,2008,29(5):418-419.
[10] 胡爱武.元素镧和氟对动物免疫和内分泌的影响[D].合肥:安徽医科大学,2007.
[11] 陈兴安,贺秋晨,关腾,等.小剂量柠檬酸稀土对小鼠中性多形核白细胞吞噬功能的影响[J].中国稀土学报,1995,13(1):70-73.
[12] 孙静,秦新英,刘会芳.氯化铒和氯化镝对小鼠免疫细胞作用的体外实验[J].河北大学学报(自然科学版),2012,32(3):281-285.
[13] 崔焕波,艾金霞.SBHL对小鼠免疫功能影响的实验研究[J].中国实验诊断学,2011,15(11):1804-1806.