基于网络药理学的黄精抗糖尿病作用机制研究
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摘要
目的:运用网络药理学方法探讨黄精抗糖尿病作用的分子机制。方法:通过TCMSP数据库获取黄精的活性成分,通过HIT和TTD数据库筛选黄精的作用靶点,建立靶点数据集,OMIM数据库筛选糖尿病相关的靶点,PPI数据库构建黄精和糖尿病的交互靶点,利用Cytoscape软件构建"成分-靶点-疾病"交互网络图,并通过DAVID数据库中GO和KEGG通路分析靶点功能及信号通路。结果:筛选出黄精12个活性成分,且涉及胰岛素受体、半胱氨酸蛋白酶-3、诱导型一氧化氮合酶、雌激素受体等17个靶点,通过调节PI3K-Akt信号通路、AMPK信号通路、胰岛素抵抗、胰岛素信号通路和Ⅱ型糖尿病等信号通路发挥抗糖尿病作用。结论:黄精抗糖尿病具有多成分、多靶点、多途径的作用特点,为进一步开展黄精抗糖尿病作用的分子机制研究提供了新思路和科学依据。
Objective: To explore the anti-diabetic molecular mechanism of Polygonati Rhizoma by network pharmacology.Methods: The active components of Polygonati Rhizoma were obtained from TCMSP database. The target of Polygonati Rhizoma was screened by HIT and TTD databases; target dataset was established; OMIM database was used to screen the diabetes-related targets,and PPI database was used to construct the interactive targets for Polygonati Rhizoma and diabetes. Cytoscape software was used to construct a "component-target-disease" interactive network map, and the target function and signaling pathway were analyzed by the GO and KEGG pathways in the DAVID database. Results: The 12 active components of Polygonati Rhizoma were screened, and the active components of Polygonati Rhizoma were involved in 17 targets including insulin receptor, Caspase-3, inducible nitric oxide synthase and estrogen receptor, etc. Anti-diabetic effects of Polygonati Rhizoma was regulated by multiple signaling pathways including PI3 K-Akt signaling pathway, AMPK signaling pathway, Insulin resistance, Insulin signaling pathway and Type II diabetes mellitus. Conclusion: This study demonstrates the multi-component-multi-target-multi-pathway characteristics of Polygonati Rhizoma,which provides a new idea and scientific basis for further research on the molecular mechanism of anti-diabetic effect of Polygonati Rhizoma.
Objective: To explore the anti-diabetic molecular mechanism of Polygonati Rhizoma by network pharmacology.Methods: The active components of Polygonati Rhizoma were obtained from TCMSP database. The target of Polygonati Rhizoma was screened by HIT and TTD databases; target dataset was established; OMIM database was used to screen the diabetes-related targets,and PPI database was used to construct the interactive targets for Polygonati Rhizoma and diabetes. Cytoscape software was used to construct a "component-target-disease" interactive network map, and the target function and signaling pathway were analyzed by the GO and KEGG pathways in the DAVID database. Results: The 12 active components of Polygonati Rhizoma were screened, and the active components of Polygonati Rhizoma were involved in 17 targets including insulin receptor, Caspase-3, inducible nitric oxide synthase and estrogen receptor, etc. Anti-diabetic effects of Polygonati Rhizoma was regulated by multiple signaling pathways including PI3 K-Akt signaling pathway, AMPK signaling pathway, Insulin resistance, Insulin signaling pathway and Type II diabetes mellitus. Conclusion: This study demonstrates the multi-component-multi-target-multi-pathway characteristics of Polygonati Rhizoma,which provides a new idea and scientific basis for further research on the molecular mechanism of anti-diabetic effect of Polygonati Rhizoma.
引文
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[3]公惠玲,李卫平,尹艳艳,等.黄精多糖对链脲菌素糖尿病大鼠降血糖作用及其机制探讨[J].中国中药杂志,2009,34(9):1149-1154.
[4]公惠玲,尹艳艳,李卫平,等.黄精多糖对四氧嘧啶诱导的糖尿病小鼠血糖和抗氧化作用的影响[J].安徽医科大学学报,2008,43(5):538-540.
[5]徐茂红,李卫平,公惠玲.黄精多糖对四氧嘧啶糖尿病模型小鼠糖脂代谢的影响[J].安徽医药,2009,13(3):263-265.
[6]王建新.黄精降糖降脂作用的实验研究[J].中国中医药现代远程教育,2009,7(1):93-94.
[7]陈婷婷,王国贤,付婷婷,等.黄精多糖对Ⅰ型糖尿病大鼠心肌炎症的保护作用[J].中药药理与临床,2015,31(4):86-90.
[8]付婷婷,王国贤,陈婷婷,等.黄精多糖对糖尿病肾病大鼠肾脏的保护作用[J].中药药理与临床,2015,31(4):123-126.
[9]HOPKINS A L.Network pharmacology[J].Nat Biotechnol,2007,25(10):1110-1111.
[10]李泮霖,苏薇薇.网络药理学在中药研究中的最新应用进展[J].中草药,2016,47(16):2938-2942.
[11]刘丹,朱靖博,王永华,等.基于网络药理学的银杏叶提取物治疗痛风潜在作用机制初探[J].中草药,2016,47(15):2693-2700.
[12]郝俊杰,王涛.基于网络药理学的桂枝甘草汤治疗心律失常的作用机制研究[J].大理大学学报,2017,2(10)1-4.
[13]DENNIS G,SHERMAN B T,HOSACK D A,et al.DAVID:Database for Annotation,Visualization,and Integrated Discovery[J].Genome Biol,2003,4:R60.
[14]郭阳艳,刘萌萌,杨晓华,等.黄芩素对大鼠胰岛素分泌的作用及机制研究[J].中国药理学通报,2018,34(6):820-824.
[15]GUPTA R,SHARMA A K,DOBHAL M P,et al.Antidiabetic and antioxidant potential ofβ-sitosterol in streptozotocin-induced experimental hyperglycemia[J].J Diabetes,2011,3(1):29-37.
[16]陈建国.中医现代化的现状、现存问题与对策[J].大家健康:中旬刊,2013,7(8):183-184.
[17]王艺,彭国庆,江新泉,等.黄精多糖对糖尿病大鼠模型的保护机制研究[J].中医药导报,2017,23(2):8-16.