葡萄汁有孢汉逊酵母对采后草莓灰霉病抗性诱导机理研究
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摘要
以采后‘红颜’草莓果实为试验材料,通过接种葡萄汁有孢汉逊酵母(Hanseniaspora uvarum)、灰葡萄孢(Botrytis cinerea)、先接种H. uvarum后挑战接种B. cinerea来探究H. uvarum对草莓采后灰霉病的生防效果及对草莓抗性诱导的相关机理。结果显示,H. uvarum可短时间内在果实伤口部位定殖并大量繁殖,对草莓灰霉病有较好的防治效果,果实病斑直径显著减小。基因表达结果显示,先接种H. uvarum后挑战接种B. cinerea处理草莓果实的水杨酸(Salicylic acid)信号通路相关基因PR1、PR5、GLU、WRKY1、NPR1和PAL表达丰度在接种后12～24 h内显著上调,并高于H. uvarum和B. cinerea单独处理,但对茉莉酸(Jasmonic acid)信号通路标志基因PDF1和JAR1无影响;先接种H. uvarum后挑战接种B. cinerea处理可以短时间内提升草莓果实抗性相关酶SOD、APX、CAT和PAL的活性;接种H. uvarum可以延缓果实冷藏期间的劣变速度,维持较高的品质。
In this experiment,‘Hongyan'strawberry fruits were used as the experimental materials to explore the biocontrol efficacy and mechanism of inducing strawberry resistance by inoculation with Hanseniaspora uvarum,Botrytis cinerea,H. uvarum challenged with B. cinerea. The results showed that H. uvarum can compete for nutrition and space in the wound by large reproduction,having good biocontrol effect on the gary mold of strawberry,and the lesion diameter of strawberry decreased significantly in H. uvarum treatment. Results of gene expression abundance indicated that the WRKY1,NPR1,PAL,PR1,PR5,GLU were up-regulated within 12 h to 24 h of H. uvarum + B. cinerea treatment,which are the marker genes of SA signaling pathway,and the up-regulation was higher than that of H. uvarum or B. cinerea treatment alone. But the PDF1 and JAR1 genes of JA signaling pathway were not accumulated in each treatment. H. uvarum challenged with B. cinerea treatment can increase the SOD,APX,PAL and CAT enzyme activity in a short time,which are involved in plant defense. The H. uvarum treatment also can delay the deterioration of fruit and maintain high quality during the cold storage period.
In this experiment,‘Hongyan'strawberry fruits were used as the experimental materials to explore the biocontrol efficacy and mechanism of inducing strawberry resistance by inoculation with Hanseniaspora uvarum,Botrytis cinerea,H. uvarum challenged with B. cinerea. The results showed that H. uvarum can compete for nutrition and space in the wound by large reproduction,having good biocontrol effect on the gary mold of strawberry,and the lesion diameter of strawberry decreased significantly in H. uvarum treatment. Results of gene expression abundance indicated that the WRKY1,NPR1,PAL,PR1,PR5,GLU were up-regulated within 12 h to 24 h of H. uvarum + B. cinerea treatment,which are the marker genes of SA signaling pathway,and the up-regulation was higher than that of H. uvarum or B. cinerea treatment alone. But the PDF1 and JAR1 genes of JA signaling pathway were not accumulated in each treatment. H. uvarum challenged with B. cinerea treatment can increase the SOD,APX,PAL and CAT enzyme activity in a short time,which are involved in plant defense. The H. uvarum treatment also can delay the deterioration of fruit and maintain high quality during the cold storage period.
引文
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Fan Wei-hua,Dong Xin-nian.2002.In vivo interaction between NPR1 and transcription factor TGA2 leads to salicylic acid-mediated gene activation in Arabidopsis.Plant Cell,14(6):1377.
Gao Pan,Qin Jia-xing,Li De-long,Zhou Shan-yue.2018.Inhibitory effect and possible mechanism of a Pseudomonas strain QBA5 against gray mold on tomato leaves and fruits caused by Botrytis cinerea.PLoS ONE,13(1):e0190932.
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Jiao Wen-xiao,Li Xiang-xin,Wang Xiao-mei.2018.Chlorogenic acid induces resistance against Penicillium expansum in peach fruit by activating the salicylic acid signaling pathway.Food Chemistry,260:274-282.
Jiang Chun-hao,Liao Meng-jie,Wang Hong-kai,Zheng Ming-zi,Xu Jian-jun,Guo Jian-hua.2018.Bacillus velezensis,a potential and efficient biocontrol agent in control of pepper gray mold caused by Botrytis cinerea.Biological Control,126:147-157.
Jiang Chun-hao,Wu Fang,Xie Ping,Ke Hong-jiao,Yu Yi-yang,Guo Jian-hua.2015.Study on screening and antagonistic mechanisms of Bacillus amyloliquefaciens 54 against bacterial fruit blotch(BFB)caused by Acidovorax avenae subsp.citrulli.Microbiological Research,170:95-104.
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Li Dei-quan,Qian Ya-ming,Zhou Ming-ming,Tan Rong,Deng Zi-fa,Yuan Su-xia.2016.Mechanism of marine bacterial NH-8 against strawberry gray mold and its antibacterial substances.Journal of Plant Protection,43(2):42-48.(in Chinese)李德全，钱亚明，周鸣鸣，谈蓉，邓自发，袁素霞.2016.海洋细菌NH-8防治草莓灰霉病机理及其抗菌物质分析.植物保护学报，43(2):42-48.
Li Wan-hai.2016.Biocontrol of postharvest green mold decay of citrus by Hanseniaspora uvarum in combinaion with phosphatidylchline and the possible mechanisms involveds[M.D.Dissertation].Zhenjiang:Jiangsu University.(in Chinese)李万海.2016.葡萄汁有孢汉逊酵母结合卵磷脂对柑橘采后绿霉病的生物防治及其机制研究[硕士论文].镇江：江苏大学.
Lian Qin-gui,Zhang Jing,Gan Liang.2017.The biocontrol efficacy of Streptomyces pratensis LMM15 on Botrytis cinerea in tomato.Biomed Res Int,(1):1-11.
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