高效液相色谱法测定化妆品中邻伞花烃-5-醇
|
摘要
建立化妆品中邻伞花烃-5-醇含量的高效液相色谱检测方法。样品用甲醇超声提取15 min,过0.22μm滤膜,以X-bridge C_(18)为色谱柱,乙腈–水(60∶40)为流动相,流量为1.0 mL/min,柱温为30℃,检测波长为280 nm,采用二极管阵列检测器检测。邻伞花烃-5-醇的质量浓度在1.0～100.0 mg/L范围内与色谱峰面积呈良好的线性关系,相关系数为0.999 9,检出限为3.0 mg/kg,定量限为10.0 mg/kg。样品加标回收率为93.7%～98.0%,测定结果的相对标准偏差为1.20%～2.09%(n=6)。该方法简单,灵敏度高且重复性好,适用于化妆品中邻伞花烃-5-醇的测定。
A method for the determination of 4-isopropyl-m-cresol in cosmetics by high performance liquid chromatography was established. Samples were extracted by methanol for 15 min, ?ltered through 0.22 μm membrane,X-bridge C_(18) as chromatographic column, acetonitrile–water(60∶40) as mobile phase, ?ow rate was 1.0 mL/min, column temperature was 30℃, detection wavelength was 280 nm, and detection by diode array detector. The mass concentration of 4-isopropyl-m-cresol in the range of 1.0–100.0 mg/L showed a good linear relationship with the peak area,the correlation coef?cient was 0.999 9, the detection limit was 3.0 mg/kg and the quantitative limit was 10.0 mg/kg.The recoverie were 93.7%–98.0%, and the relative standard deviations were 1.20%–2.09%(n=6). The method is simple,sensitive and reproducible, and it is suitable for the determination of 4-isopropyl-m-cresol in cosmetics.
A method for the determination of 4-isopropyl-m-cresol in cosmetics by high performance liquid chromatography was established. Samples were extracted by methanol for 15 min, ?ltered through 0.22 μm membrane,X-bridge C_(18) as chromatographic column, acetonitrile–water(60∶40) as mobile phase, ?ow rate was 1.0 mL/min, column temperature was 30℃, detection wavelength was 280 nm, and detection by diode array detector. The mass concentration of 4-isopropyl-m-cresol in the range of 1.0–100.0 mg/L showed a good linear relationship with the peak area,the correlation coef?cient was 0.999 9, the detection limit was 3.0 mg/kg and the quantitative limit was 10.0 mg/kg.The recoverie were 93.7%–98.0%, and the relative standard deviations were 1.20%–2.09%(n=6). The method is simple,sensitive and reproducible, and it is suitable for the determination of 4-isopropyl-m-cresol in cosmetics.
引文
[1]秦振伟,施云龙,晓庆军,等.一种2-甲基-4-异丙基苯酚的制备方法:CN103044205A[P].2013-04-17.
[2]A·阿克斯,D·布拉肖,F·利珀特,等.包含4-异丙基-2甲基苯酚和锌离子的抗菌组合:CN102378627A[P].2012-03-14.
[3]YANG J J.4-Isopropyl-3-methylphenol for the treatment of inflammation:US20120034312A1[P].2012-02-09.
[4]王娟,于文.高效液相色谱法测定洗手液中的3-甲基-4-异丙基苯酚的含量[J].工业与公共设施清洁,2016(11):38-41.
[5]BURNETT G R,STEPHEN A S,PIZZEY R L,et al.In vitro effects of novel toothpaste actives on components of oral malodour[J].International dental journal,2011,61(3):67-73.
[6]PIAAEY R L,MARQUIS R E,BRADSHAW D J.Antimicrobial effects of o-cymen-5-ol and zinc,alone&in combination in simple solutions and toothpaste formulations[J].International dental journal,2011,61(3):33-40.
[7]谢晓芳,陈敏珊,李林.葡萄柚提取物/O-伞花烃-5-醇在牙膏中的应用及其杀菌速率的评价[J].口腔护理用品工业,2017,27(1):21-24.
[8]陈敏珊,李林,李平.天然提取物的抑菌研究及其在口腔护理产品中的应用[J].口腔护理用品工业,2016,26(4):16-20.
[9]李英,刘丽,刘志红.气相色谱-质谱法测定化妆品中多种防腐剂[J].色谱,2003,21(2):170-173.
[10]詹家芬,陆舍铭,曲国福,等.气相色谱/质谱法分析检测莳萝酊的挥发成分[J].精细化工,2007,24(8):805-819.
[11]孙宗喜,吕晓慧,徐桂花,等.甘肃产柴胡挥发油化学成分GC-MS分析[J].中国实验方剂学杂志,2012,18(9):75-78.
[12]李晨,黄纪国,路泰良,等.陕西荚蒾叶挥发油化学成分GC-MS分析及抗菌活性研究[J].中药材,2013,36(12):1 962-1 966.
[13]陈欣,陈鹏.RP-HPLC测定麝香草酚的含量及有关物质[J].华西药学杂志,2012,27(1):102-103.
[14]王静.高效液相色谱法测定复方水杨酸麝香草酚搽剂中樟脑和麝香草酚含量[J].中国药业,2015,24(2):44-45.
[15]陈丹,李柯,胡薏冰,等.高效液相色谱法测定显脉旋覆花药材中麝香草酚和异丁酸百里香酯的含量[J].中国民族民间医药,2012(21):45-48.
[2]A·阿克斯,D·布拉肖,F·利珀特,等.包含4-异丙基-2甲基苯酚和锌离子的抗菌组合:CN102378627A[P].2012-03-14.
[3]YANG J J.4-Isopropyl-3-methylphenol for the treatment of inflammation:US20120034312A1[P].2012-02-09.
[4]王娟,于文.高效液相色谱法测定洗手液中的3-甲基-4-异丙基苯酚的含量[J].工业与公共设施清洁,2016(11):38-41.
[5]BURNETT G R,STEPHEN A S,PIZZEY R L,et al.In vitro effects of novel toothpaste actives on components of oral malodour[J].International dental journal,2011,61(3):67-73.
[6]PIAAEY R L,MARQUIS R E,BRADSHAW D J.Antimicrobial effects of o-cymen-5-ol and zinc,alone&in combination in simple solutions and toothpaste formulations[J].International dental journal,2011,61(3):33-40.
[7]谢晓芳,陈敏珊,李林.葡萄柚提取物/O-伞花烃-5-醇在牙膏中的应用及其杀菌速率的评价[J].口腔护理用品工业,2017,27(1):21-24.
[8]陈敏珊,李林,李平.天然提取物的抑菌研究及其在口腔护理产品中的应用[J].口腔护理用品工业,2016,26(4):16-20.
[9]李英,刘丽,刘志红.气相色谱-质谱法测定化妆品中多种防腐剂[J].色谱,2003,21(2):170-173.
[10]詹家芬,陆舍铭,曲国福,等.气相色谱/质谱法分析检测莳萝酊的挥发成分[J].精细化工,2007,24(8):805-819.
[11]孙宗喜,吕晓慧,徐桂花,等.甘肃产柴胡挥发油化学成分GC-MS分析[J].中国实验方剂学杂志,2012,18(9):75-78.
[12]李晨,黄纪国,路泰良,等.陕西荚蒾叶挥发油化学成分GC-MS分析及抗菌活性研究[J].中药材,2013,36(12):1 962-1 966.
[13]陈欣,陈鹏.RP-HPLC测定麝香草酚的含量及有关物质[J].华西药学杂志,2012,27(1):102-103.
[14]王静.高效液相色谱法测定复方水杨酸麝香草酚搽剂中樟脑和麝香草酚含量[J].中国药业,2015,24(2):44-45.
[15]陈丹,李柯,胡薏冰,等.高效液相色谱法测定显脉旋覆花药材中麝香草酚和异丁酸百里香酯的含量[J].中国民族民间医药,2012(21):45-48.