C-myc和LIN28B蛋白及其mRNA在胃癌组织和相应癌旁正常组织中的表达及其与胃癌发生发展的相关性
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摘要
目的分析反式激活活性蛋白(C-myc)与LIN28B mRNA、蛋白在胃癌组织和相应癌旁正常组织中的表达及其与胃癌发生发展的相关性。方法通过Western blot法对60例胃癌组织和相应癌旁正常组织中C-myc、LIN28B蛋白表达水平进行检测,实时荧光定量PCR法测定C-myc、LIN28B mRNA表达水平,统计分析C-myc、LIN28B蛋白表达与胃癌临床病理参数的关系。结果 C-myc、LIN28B蛋白在胃癌组织中的表达水平较癌旁正常组织均明显升高(P<0. 01); C-myc、LIN28B mRNA在胃癌组织中的表达水平较癌旁正常组织均明显升高(P <0. 01)。C-myc蛋白表达与胃癌TNM分期、浸润深度及淋巴结转移均密切相关(P <0. 05); LIN28B蛋白表达与胃癌TNM分期、分化程度、浸润深度及淋巴结转移均密切相关(P <0. 05)。结论 C-myc、LIN28B蛋白及其mRNA均高表达于胃癌组织,二者与胃癌TNM分期、浸润深度及淋巴结转移均密切相关,可能共同参与胃癌的病理过程。
Objective To analyze the expression of trans activator protein( C-myc) and LIN28 B mRNA and protein in gastric cancer tissues and adjacent normal tissues and correlation with the occurrence and development of gastric cancer. Methods The Western blot method was used to detect the level of C-myc and LIN28 B protein expression in 60 cases of gastric cancer tissues and adjacent normal tissues,the expression levels of C-myc and LIN28 B mRNA were detected by the real-time fluorescence quantitative PCR method. The correlation between C-myc,LIN28 B protein expression and clinicopathological parameters of gastric cancer was statistically analyzed. Results The expression levels of C-myc and LIN28 B protein in gastric cancer tissues were significantly higher than those in adjacent normal tissues( P < 0. 01). The expression levels of C-myc and LIN28 B mRNA in gastric cancer tissues were significantly higher than those in adjacent normal tissues( P < 0. 01). C-myc protein expression was closely correlated with TNM staging,depth of invasion and lymph node metastasis of gastric cancer( P < 0. 05). LIN28 B protein expression was closely correlated with TNM staging,differentiation,invasion depth and lymph node metastasis of gastric cancer( P < 0. 05).Conclusion C-myc,LIN28 B protein and mRNA are all highly expressed in gastric cancer tissues,which are closely correlated with TNM stage,depth of invasion and lymph node metastasis,and may participate in the pathological process of gastric cancer.
Objective To analyze the expression of trans activator protein( C-myc) and LIN28 B mRNA and protein in gastric cancer tissues and adjacent normal tissues and correlation with the occurrence and development of gastric cancer. Methods The Western blot method was used to detect the level of C-myc and LIN28 B protein expression in 60 cases of gastric cancer tissues and adjacent normal tissues,the expression levels of C-myc and LIN28 B mRNA were detected by the real-time fluorescence quantitative PCR method. The correlation between C-myc,LIN28 B protein expression and clinicopathological parameters of gastric cancer was statistically analyzed. Results The expression levels of C-myc and LIN28 B protein in gastric cancer tissues were significantly higher than those in adjacent normal tissues( P < 0. 01). The expression levels of C-myc and LIN28 B mRNA in gastric cancer tissues were significantly higher than those in adjacent normal tissues( P < 0. 01). C-myc protein expression was closely correlated with TNM staging,depth of invasion and lymph node metastasis of gastric cancer( P < 0. 05). LIN28 B protein expression was closely correlated with TNM staging,differentiation,invasion depth and lymph node metastasis of gastric cancer( P < 0. 05).Conclusion C-myc,LIN28 B protein and mRNA are all highly expressed in gastric cancer tissues,which are closely correlated with TNM stage,depth of invasion and lymph node metastasis,and may participate in the pathological process of gastric cancer.
引文
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[9] Teng R,Hu Y,Zhou J,et al. Overexpression of Lin28 decreases the chemosensitivity of gastric cancer cells to oxaliplatin,paclitaxel,doxorubicin,and fluorouracil in part via microRNA-107[J]. Plo S one,2015,10(12):e0143716.
[10]梁海海,李天宇,解岩,等. Lin28B/let-7d环路调控成纤维细胞功能参与肺纤维化发生[J].中国药理学通报,2017,33(2):175-179,180.
[11]白丽鹏,陈翀,刘妍,等. LIN28B调控miRNAs维持MCF-7肿瘤干细胞的干性特征[J].基础医学与临床,2015,35(6):754-760.
[12]屈婷婷. LIN28B的研究进展[J].医学分子生物学杂志,2013,10(3):177-179,183.
[13] Lin S,Gregory RI. MicroRNA biogenesis pathways in cancer[J]. Nat Rev Cancer,2015,15(6):321-333.
[14]韩威,苏一军,朱云芬,等.文昌鸡性成熟启动前后下丘脑Cmyc/LIN28B/let-7a通路表达规律分析[J].畜牧兽医学报,2016,47(4):709-715.
[15] Wang T,Wang G,Hao D,et al. Aberrant regulation of the LIN28A/LIN28B and let-7 loop in human malignant tumors and its effects on the hallmarks of cancer[J]. Mol Cancer,2015,14:125.
[2]郭志远,付吕平,付志国,等.重组人血管内皮抑制素对小鼠胃癌C-myc,bFGF表达的影响[J].标记免疫分析与临床,2015,22(9):916-920.
[3]刘龑航,刘映辉,董锡钓,等. Wnt2、APC及C-myc在胃癌中的表达[J].中国老年学杂志,2013,33(23):5838-5840.
[4]毛晓丹,谭宇思,李玉红,等.转化生长因子β1作用下绒毛膜癌JEG-3细胞C-myc mRNA表达[J].临床和实验医学杂志,2014,13(3):168-171.
[5]刘光耀,刘莉. survivin与C-myc在胃癌组织中的表达及意义[J].癌症进展,2017,15(12):1426-1429.
[6]黄国全,张才全,吴泉峰,等. F框/WD-40域蛋白7及C-myc在胃癌中的表达及临床意义[J].中华实验外科杂志,2014,31(10):2289.
[7] Song H,Xu W,Song J,et al. Overexpression of Lin28 inhibits the proliferation,migration and cell cycle progression and induces apoptosis of BGC-823 gastric cancer cells[J]. Oncol Rep,2015,33(2):997-1003.
[8]郑俊全,伊索恒,武杰,等.富含亮氨酸重复序列免疫球蛋白样蛋白1、E-Cadherin和EGFR在胃癌中的表达和临床意义[J].临床和实验医学杂志,2017,16(23):2329-2332.
[9] Teng R,Hu Y,Zhou J,et al. Overexpression of Lin28 decreases the chemosensitivity of gastric cancer cells to oxaliplatin,paclitaxel,doxorubicin,and fluorouracil in part via microRNA-107[J]. Plo S one,2015,10(12):e0143716.
[10]梁海海,李天宇,解岩,等. Lin28B/let-7d环路调控成纤维细胞功能参与肺纤维化发生[J].中国药理学通报,2017,33(2):175-179,180.
[11]白丽鹏,陈翀,刘妍,等. LIN28B调控miRNAs维持MCF-7肿瘤干细胞的干性特征[J].基础医学与临床,2015,35(6):754-760.
[12]屈婷婷. LIN28B的研究进展[J].医学分子生物学杂志,2013,10(3):177-179,183.
[13] Lin S,Gregory RI. MicroRNA biogenesis pathways in cancer[J]. Nat Rev Cancer,2015,15(6):321-333.
[14]韩威,苏一军,朱云芬,等.文昌鸡性成熟启动前后下丘脑Cmyc/LIN28B/let-7a通路表达规律分析[J].畜牧兽医学报,2016,47(4):709-715.
[15] Wang T,Wang G,Hao D,et al. Aberrant regulation of the LIN28A/LIN28B and let-7 loop in human malignant tumors and its effects on the hallmarks of cancer[J]. Mol Cancer,2015,14:125.