虎掌南星化学成分及其抑菌活性
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摘要
目的研究虎掌南星Pinellia pedatisecta Schott的化学成分及其抑菌活性。方法虎掌南星氯仿提取物采用硅胶、ODS、MCI进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。平板法筛选最低抑菌浓度。结果从中分离得到10个化合物,分别鉴定为β-谷甾醇(1)、邻苯二甲酸丁二酯(2)、胡萝卜苷(3)、(2S)-1-O-十六碳酰基-2-O-亚油酰基-3-O-β-D-半乳糖基甘油(4)、diacygalactolipidⅠ(5)、(2S)-1-O-十八碳酰基-2-O-亚油酰基-3-O-β-D-半乳糖基甘油(6)、(2S)-1,2-双-亚油酰基-3-O-β-D-半乳糖基甘油(7)、(2S)-1-O-十六碳酰基-2-O-十四碳酰基-3-O-β-D-半乳糖基甘油(8)、spongilipid(9)、丙三醇(10)。对于金黄色葡萄球菌、黑曲霉菌、幽门螺旋杆菌,化合物4、7的最低抑菌浓度分别为20、30、50μg/mL,25、50、150μg/mL。结论所有化合物均为首次从该植物中分离得到,化合物4、7有较强的抑菌活性。
AIM To study the chemical constituents from Pinellia pedatisecta Schott and their antibacterial activites.METHODS The chloroform extract from P. pedatisecta was isolated and purified by silica, ODS and MCI,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The minimal inhibitory concentration(MICs) of pure phytochemicals was evaluated by agar dilution.RESULTS Ten compounds were isolated and identified as β-sterol(1), di-butyl phthalate(2), daucosterol(3), l-O-hexadecanoyl-2(S)-O-(9Z, 12Z-octadecadienoyl)-3-O-β-D-galacatopyranosyl-glycerol(4), diacygalactolipid Ⅰ(5), 2(S)-1-O-(octadecanoyl)-2-O-(9Z,12Z-octadecadienoyl)-3-O-β-D-galactopyranoyl-glycerol(6),(2S)-1,2-O-(9Z,12Z-di-octadica-dienoylglyceryl)-3-O-β-D-galactopyranoside(7),1-O-phalmitoyl-2(S)-O-myristoyl-glyceryl-3-O-β-D-galactopyranoside(8), spongilipid(9), glycerine(10). Compounds 4,7 inhibited growths of Staphylococcus aureus, Aspergillus niger, Helicobacter pylori with MICs of 20, 30,50 mg/mL and 25, 50, 150 mg/mL,respectively.CONCLUSION All the compounds are isolatd from this plant for the first time, and compounds 4,7 have strong antibacterial activites.
AIM To study the chemical constituents from Pinellia pedatisecta Schott and their antibacterial activites.METHODS The chloroform extract from P. pedatisecta was isolated and purified by silica, ODS and MCI,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The minimal inhibitory concentration(MICs) of pure phytochemicals was evaluated by agar dilution.RESULTS Ten compounds were isolated and identified as β-sterol(1), di-butyl phthalate(2), daucosterol(3), l-O-hexadecanoyl-2(S)-O-(9Z, 12Z-octadecadienoyl)-3-O-β-D-galacatopyranosyl-glycerol(4), diacygalactolipid Ⅰ(5), 2(S)-1-O-(octadecanoyl)-2-O-(9Z,12Z-octadecadienoyl)-3-O-β-D-galactopyranoyl-glycerol(6),(2S)-1,2-O-(9Z,12Z-di-octadica-dienoylglyceryl)-3-O-β-D-galactopyranoside(7),1-O-phalmitoyl-2(S)-O-myristoyl-glyceryl-3-O-β-D-galactopyranoside(8), spongilipid(9), glycerine(10). Compounds 4,7 inhibited growths of Staphylococcus aureus, Aspergillus niger, Helicobacter pylori with MICs of 20, 30,50 mg/mL and 25, 50, 150 mg/mL,respectively.CONCLUSION All the compounds are isolatd from this plant for the first time, and compounds 4,7 have strong antibacterial activites.
引文
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[16] 艾双艳,李卫国,冯亚东,等. 全蝎化学成分的研究[J]. 中成药,2017,39(8):1639-1641.
[2] 汪荣斌,刘晓龙,王存琴,等. 天南星的本草考证与药用品种调查[J].中药材,2010,33(7):1182-1185.
[3] 徐树楠,朱兵占. 神农本草经[M]. 石家庄:河北科学技术出版社,1996:119.
[4] 苏颂,尚志钧. 图经本草[M].合肥:安徽科学技术出版社,1994:266,309.
[5] 卢多逊,尚志钧. 开宝本草[M]. 合肥:安徽科学技术出版社,1998:252.
[6] 唐慎微. 证类本草[M]. 北京:华夏出版社,1993:281,310.
[7] 李彦文,李志勇,郭庆梅,等. 虎掌南星的本草考证[J]. 现代中药研究与实践,2012, 26(4):18-20.
[8] 曾孝杰,王国才,吴霞,等. 山沉香化学成分研究[J].中草药,2013,44(13):1721-1725.
[9] Byeng W S, Jung C K, Sang M L, et al. New diacylgalactolipids from the marine Cyanophycean Microalga Oscillatoria sp.[J]. Bull Korean Chem Soc, 2000, 21(11):1138-1140.
[10] Jung J H, Lee H, Kang S S.Diacylglycerylgalactosides from Arisaema amurense [J].Phytochemistry, 1996, 42(2):447.
[11] Fan G J, Kim S H, Han B H, et al. Glyceroglycolipids, a novel class of platelet-activating factor antagonists from Kalimeris indica[J]. Phytochem Lett, 2008, 1(4):207-210.
[12] Son B W, Kim J C, Lee S M, et al. New diacylgalactolipids from the marine Cyanophycean Microalga Oscillatoria sp.[J]. Bull Korean Chem Soc,2000, 21(11):1138-1140.
[13] Kiem V P, Minh V C, Nhiem X N, et al. Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa[J]. Arch Pharm Res, 2012,35(12):2135-2142.
[14] Murakami N,Morimoto T,Imamura H,et al.Studies of glycolipids.Ⅲ.glyceroglycolipids from an axenically cultured cyanobacterium,Phormidium tenue [J].Chem Pharm Bull (Tokyo),1991,39(9):2277-2281.
[15] Pettit G R, Bond T J, Herald D L, et al. Isolation and structure of spongilipid from the republic of Singapore marine porifera spongia cf.lispida [J]. Can J Chem,1997, 75(6):920-925.
[16] 艾双艳,李卫国,冯亚东,等. 全蝎化学成分的研究[J]. 中成药,2017,39(8):1639-1641.