采用改良磷钼钨酸-干酪素法测定闽产盐肤木根中总鞣质含量
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摘要
目的:建立舒冠通糖浆原药材盐肤木根中总鞣质的含量测定方法,并对产自福建的该药材进行鞣质含量的测定。方法:以《中华人民共和国药典》2015版四部中鞣质含量测定法(磷钼钨酸-干酪素法)为基础,在显色温度、显色时间、干酪素的用量及处理方法等方面进行改良,确定了含量测定方法,并用该法测定样品中总鞣质含量。结果:通过对鞣质含量测定各环节的考察,确定了原药材鞣质含量测定方法,并进行了方法学验证,没食子酸在1.00～12.00μg/mL与吸光度呈良好的线性关系,r=0.9985,操作重复性良好,RSD=2.89%,闽产盐肤木根中鞣质含量为22.1 mg·g-1(n=6,RSD=2.39%)。结论:经方法学验证,该方法可用于测定闽产盐肤木根中总鞣质的含量。
Objective:To establish the method for determination of total tannis of the root of Rhus chinensis from Fujian province and determine its content. Methods:An optimized method with which the content of total tannis was finally determined was developed based on the phosphomolybdium tungstic acid-casein reaction reported in Pharmacopoeia of the People's Republic of China 2015,volumeⅣ. Incubation time and amount of the casein were modified. Results:The modified strategy achieved good results. The gallic acid had good linear relationship in the range of 1.00-12.00 μg/mL(r=0.9985). The repeatability was also satisfactory,with the RSD of 2.89%,total tannis of the root of Rhus chinensis from Fujian province was 22.1 mg·g-1(n=6,RSD=2.39%). Conclusion:According to the methodology validation,this method can be applied to the determination of the total tannis of the Rhus chinensis root from Fujian province.
Objective:To establish the method for determination of total tannis of the root of Rhus chinensis from Fujian province and determine its content. Methods:An optimized method with which the content of total tannis was finally determined was developed based on the phosphomolybdium tungstic acid-casein reaction reported in Pharmacopoeia of the People's Republic of China 2015,volumeⅣ. Incubation time and amount of the casein were modified. Results:The modified strategy achieved good results. The gallic acid had good linear relationship in the range of 1.00-12.00 μg/mL(r=0.9985). The repeatability was also satisfactory,with the RSD of 2.89%,total tannis of the root of Rhus chinensis from Fujian province was 22.1 mg·g-1(n=6,RSD=2.39%). Conclusion:According to the methodology validation,this method can be applied to the determination of the total tannis of the Rhus chinensis root from Fujian province.
引文
[1]中国科学院中国植物志编辑委员会.中国植物志[S].北京:科学出版社,1980:100.
[2]福建省科学技术委员会.福建植物志:第三卷[S].福州:福建科学技术出版社,1988:244.
[3]叶聚荣,林大杰,郑幼兰,等.盐肤木对冠脉循环的作用及其毒性[J].中国药学杂志,1981(1):50.
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[16]Smeriglio A,Barreca D,Bellocco E,et al.Proanthocyanidins and hydrolysable tannins:occurrence,dietary intake and pharmacological effects[J].Br J Pharmacol,2017,174(11):1244-1262.
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[19]Quifer-Rada P,Choy Y Y,Calvert C C,et al.Use of metabolomics and lipidomics to evaluate the hypocholestreolemic effect of proanthocyanidins from grape seed in a pig model[J].Mol Nutr Food Res,2016,60(10):2219-2227.
[20]Draijer R,de Graaf Y,Slettenaar M,et al.Consumption of a polyphenol-rich grape-wine extract lowers ambulatory blood pressure in mildly hypertensive subjects[J].Nutrients,2015(7):3138-3153.
[21]Pons Z,Guerrero L,Margalef M,et al.Effect of low molecular grape seed proanthocyanidins on blood pressure and lipid homeostasis in cafeteria diet-fed rats[J].J Physiol Biochem,2014(70):629-637.
[22]国家药典委员会.中华人民共和国药典(四部)[S].2015年版.北京:中国医药科技出版社,2015:38,323.
[23]谢坤,唐丽,张婉,等.磷钼钨酸/干酪素法测定蒙药阿给炮制前后的鞣质含量[J].中药材,2009,32(10):1517-1519.
[24]谢道刚,宋光志,刘静.鞣质含量测定法(中国药典2005版一部附录XB)方法学验证[J].世界科学技术-中医药现代化,2006,8(6):50-53.
[2]福建省科学技术委员会.福建植物志:第三卷[S].福州:福建科学技术出版社,1988:244.
[3]叶聚荣,林大杰,郑幼兰,等.盐肤木对冠脉循环的作用及其毒性[J].中国药学杂志,1981(1):50.
[4]张一意,欧阳恒慧,林观惠.从血液流变学探索舒冠通糖浆的作用原理[J].中成药,1988(7):27-28.
[5]沈亚英.治疗冠心病新药--舒冠通糖浆[J].中国药学杂志,1980,15(5):28.
[6]福建省第一医院.盐肤木治疗冠心病研究[J].新医学,1978(4):198.
[7]郑义信.舒冠通糖浆[J].福建医药杂志,1980(2):71.
[8]高洁莹,龚力民,刘平安,等.盐肤木属植物研究进展[J].中国实验方剂学杂志,2015,21(8):215-218.
[9]赵军,崔承彬,蔡兵,等.国产盐肤木属植物的研究进展[J].解放军药学学报,2006,22(1):48-51.
[10]Djakpo O,Yao W.Rhus chinensis and Galla Chinensis-folklore to modern evidence:review[J].Phytother Res,2010,24(12):1739.
[11]TAN D,YAN QX,MA XJ.Chemical constituents of Rhus chinensis[J].Chem Nat Compounds,2015,51(3):542-543.
[12]顾琼.五种药用植物化学与抗HIV成分研究[D].昆明:中国科学院昆明植物研究所,2007.
[13]赵军.盐肤木抗肿瘤活性成分的研究[D].天津:天津大学,2006.
[14]De Jesus N Z,de Souza Falc?o H,Gomes I F,et al.Tannins,peptic ulcers and related mechanisms[J].Int J Mol Sci,2012(13):3203-3228.
[15]刘畅,金哲雄.鞣质的药理活性研究进展[J].黑龙江医药,2015,28(1):14-17.
[16]Smeriglio A,Barreca D,Bellocco E,et al.Proanthocyanidins and hydrolysable tannins:occurrence,dietary intake and pharmacological effects[J].Br J Pharmacol,2017,174(11):1244-1262.
[17]Bladé C,Aragonès G,Arola-Arnal A,et al.Proanthocyanidins in health and disease[J].Biofactors,2016,42(1):5-12.
[18]张杰,詹炳炎.鞣质的药理作用(Ⅲ)--抗氧化、降脂、降压等多种作用[J].国外医药(植物药分册),1995,10(3):99-103.
[19]Quifer-Rada P,Choy Y Y,Calvert C C,et al.Use of metabolomics and lipidomics to evaluate the hypocholestreolemic effect of proanthocyanidins from grape seed in a pig model[J].Mol Nutr Food Res,2016,60(10):2219-2227.
[20]Draijer R,de Graaf Y,Slettenaar M,et al.Consumption of a polyphenol-rich grape-wine extract lowers ambulatory blood pressure in mildly hypertensive subjects[J].Nutrients,2015(7):3138-3153.
[21]Pons Z,Guerrero L,Margalef M,et al.Effect of low molecular grape seed proanthocyanidins on blood pressure and lipid homeostasis in cafeteria diet-fed rats[J].J Physiol Biochem,2014(70):629-637.
[22]国家药典委员会.中华人民共和国药典(四部)[S].2015年版.北京:中国医药科技出版社,2015:38,323.
[23]谢坤,唐丽,张婉,等.磷钼钨酸/干酪素法测定蒙药阿给炮制前后的鞣质含量[J].中药材,2009,32(10):1517-1519.
[24]谢道刚,宋光志,刘静.鞣质含量测定法(中国药典2005版一部附录XB)方法学验证[J].世界科学技术-中医药现代化,2006,8(6):50-53.