不同氮磷比对小球藻叶绿素荧光参数及生长的影响
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摘要
以小球藻(Chlorella vulagris)为研究对象,研究不同氮磷比对其叶绿素荧光、叶绿素含量和细胞密度的影响,以期找到小球藻生长最适的氮磷比,为小球藻的规模化培养提供基础资料。结果表明:不同浓度的氮磷比对小球藻的叶绿素荧光、叶绿素含量和细胞密度有显著影响,各处理组均呈上升趋势,其中17. 30∶1处理组的最大光能转化速率F_v/F_m、潜在活力F_v/F_o、实际光能转化效率ΦPSⅡ和量子效率Yield、相对电子转化速率ETR均高于其他处理组,34. 60∶1处理组叶绿素含量和细胞密度最高,其值分别为3 231. 81μg/L和1. 07×10~7个/mL。138. 40∶1处理组上升趋势最小,且各处理组的荧光参数指标在试验后8 d开始呈下降趋势。小球藻在氮磷比为17. 30∶1生长最好。
引文
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[29]孙开明.东海原甲藻光合系统Ⅱ可变荧光对营养盐变化的响应研究[D].青岛:中国海洋大学,2015.
[30]许珍,殷大聪,陈进,等.温度和光强对4种常见水华藻叶绿素荧光特性的影响[J].长江科学院院报,2017,34(6):39-44.
[2]Pancha I,Chokshi K,George B,et al.Nitrogen stress triggered biochemical and morphological changes in the microalgae Scenedesmus sp.CCNM 1077[J].Bioresource Technology,2014,156(2):146-154.
[3]He G,Zhang J,Hu X,et al.Effect of aluminum toxicity and phosphorus deficiency on the growth and photosynthesis of oil tea(Camellia oleifera Abel.)seedlings in acidic red soils[J].Acta Physiologiae Plantarum,2010,33(4):1285-1292.
[4]王英英.不同氮磷质量浓度对太浦河四种优势藻类生长影响的研究[D].上海:上海师范大学,2016.
[5]郑杰,黄显怀,尚巍,等.不同氮磷比对藻类生长及水环境因子的影响[J].工业用水与废水,2011,42(1):12-16.
[6]Xin L,Hu H Y,Ke G,et al.Effects of different nitrogen and phosphorus concentrations on the growth,nutrient uptake,and lipid accumulation of a freshwater microalga Scenedesmus sp.[J].Bioresource Technology,2010,101(14):5494-5500.
[7]韩博平.藻类光合作用机理与模型[M].北京:科学出版社,2003.
[8]宋丽娜,郑晓宇,顾詠洁,等.磷浓度对海洋小球藻叶绿素荧光及生长的影响[J].环境污染与防治,2010,32(8):20-24.
[9]Schreiber U.Detection of rapid induction kinetics with a new type of high frequency modulated chlorophyll fluorometer[J].Photosynthesis Research,1986,9(1/2):261-272.
[10]胡丰姣,黄鑫浩,朱凡,等.叶绿素荧光动力学技术在胁迫环境下的研究进展[J].广西林业科学,2017,46(1):102-106.
[11]Lechaudel M,Urban L,Joas J.Chlorophyll fluorescence,a nondestructive method to assess maturity of mango fruits(cv.‘Cogshall’)without growth conditions bias[J].Journal of Agricultural&Food Chemistry,2010,58(13):7532-7538.
[12]Guo H,Yao J,Sun Z,et al.Effect of temperature,irradiance on the growth of the green alga Caulerpa lentillifera(Bryopsidophyceae,Chlorophyta)[J].Journal of Applied Phycology,2015,27(2):879-885.
[13]吕士如.基于藻类叶绿素荧光动力学曲线的水质监测系统的设计[D].重庆:重庆大学,2014.
[14]Miyake C,Amako K,Shiraishi N,et al.Acclimation of tobacco leaves to high light intensity drives the plastoquinone oxidation system-relationship among the fraction of open PSⅡcenters,non-photochemical quenching of Chl fluorescence and the maximum quantum yield of PSⅡin the dark[J].Plant&Cell Physiology,2009,50(4):730-43.
[15]Li C,Liu K.Analysis of photosynthesis efficiency of maize hybrids with different yield in the later growth stage[J].Acta Agronomica Sinica,2002,28.
[16]王立丰,王纪坤.叶绿素荧光动力学原理及在热带作物研究中的应用[J].热带农业科学,2013,33(11):16-23.
[17]尤鑫,龚吉蕊.叶绿素荧光动力学参数的意义及实例辨析[J].西部林业科学,2012,41(5):90-94.
[18]陈莲花,刘雷.叶绿素荧光技术在藻类光合作用中的应用[J].江西科学,2007,25(6):788-790.
[19]Holzwarth A R,Lenk D,Jahns P.On the analysis of nonphotochemical chlorophyll fluorescence quenching curves:Ⅰ.Theoretical considerations[J].Biochimica et Biophysica Acta,2013,1827(6):786-92.
[20]Zhou R,Zhao H.Seasonal pattern of antioxidant enzyme system in the roots of perennial forage grasses grown in alpine habitat,related to freezing tolerance[J].Physiologia Plantarum,2004,121(3):399-408.
[21]葛红星,陈钊,李健,等.pH和氮磷比对微小原甲藻和青岛大扁藻生长竞争的影响[J].中国水产科学,2017,24(3):587-595.
[22]雷玉新,刘耀兴,席银,等.水体中铁离子和氮磷比对藻类生长影响研究[J].生态科学,2016(1):75-78.
[23]李慧,丁刚,辛美丽,等.不同氮、磷浓度及配比对铜藻(Sargassum horneri)幼苗生长的影响[J].海洋与湖沼,2017,48(2):368-372.
[24]陈庆荣,林长顺.氮、磷对青岛大扁藻生长的影响初探[J].农民致富之友,2017(8):63-64.
[25]刘东艳,孙军,巩晶,等.不同氮、磷比例对球等鞭金藻生长的影响[J].海洋水产究,2002,23(1):29-32.
[26]李冰.氮磷营养盐与藻类生长相关性研究[D].济南:山东建筑大学,2013.
[27]陈书秀,梁英,王虎.氮磷浓度对雨生红球藻叶绿素荧光参数的影响[J].淡水渔业,2012,42(1):15-19.
[28]王昭玉.叶绿素荧光参数对氮、磷限制的响应及其在赤潮生消过程中的变化特征研究[D].青岛:中国海洋大学,2013.
[29]孙开明.东海原甲藻光合系统Ⅱ可变荧光对营养盐变化的响应研究[D].青岛:中国海洋大学,2015.
[30]许珍,殷大聪,陈进,等.温度和光强对4种常见水华藻叶绿素荧光特性的影响[J].长江科学院院报,2017,34(6):39-44.