2型糖尿病患者牙槽骨骨髓间充质干细胞生物学特性研究
|
摘要
目的:观察2型糖尿病患者牙槽骨骨髓间充质干细胞的生物学特性,为进一步探讨糖尿病影响种植体骨结合的分子机制提供实验和理论基础。方法:通过体外培养正常人和2型糖尿病患者骨髓间充质干细胞(bone mesench ymal stem cells,BMSCs),将正常人与糖尿病患者BMSCs分别分为高糖组、低糖组,检测以下指标:细胞增殖、细胞凋亡率、细胞黏附及细胞迁移;结果:BMSCs细胞在高糖环境下的增殖能力明显减弱,正常人牙槽骨来源的BMSCs细胞增殖能力优于糖尿病患者BMSCs;而糖尿病患者高糖组BMSCs的凋亡率明显高于正常人组;各组之间细胞黏附率差异无统计学意义;正常人组BMSCs迁移能力明显高于糖尿病组BMSCs;结论:2型糖尿病患者BMSCs的增殖能力较缓慢,凋亡率较高,细胞迁移能力稍弱。高糖环境容易抑制BMSCs的增殖及迁移能力。
Objective: To observe the differences characterizations of alveolar bone-derived marrow mesenchymal stem cells in normal and diabetic patients, in order to provide experimental and theoretical basis for further exploring of implant osseointegration molecular mechanism in diabetes mellitus patients. Methods: In vitro culture of bone marrow mesenchymal stem cells from normal subjects and patients with type 2 diabetes mellitus. BMSCs of normal people and diabetic patients were divided into high sugar group and low sugar group The following indexes were detected: cell proliferation, apoptosis rate, cell adhesion and cell migration ability. Results: The cell proliferation assay showed proliferation ability of BMSCs in low glucose was higher than that in high glucose both in normal and diabetic patients. The apoptosis rate of BMSCs in high group was significantly higher than that in low glucose group in diabetic patients. There was no significant difference of BMSCs apoptosis rate in these groups. There was no statistically significant difference in cell adhesion rate between each group. BMSCs migration ability of normal patients was significantly better than that of the diabetic group. Conclusions:BMSCs cell proliferation ability in diabetic patients was slower than in normal patients. BMSCs cell migration ability in diabetic patients were weaker than in normal patients. However, adipogenic differentiation potential was slightly better than normal patients. High glucose condition could suppress BMSCs cell proliferation and cell migration ability.
Objective: To observe the differences characterizations of alveolar bone-derived marrow mesenchymal stem cells in normal and diabetic patients, in order to provide experimental and theoretical basis for further exploring of implant osseointegration molecular mechanism in diabetes mellitus patients. Methods: In vitro culture of bone marrow mesenchymal stem cells from normal subjects and patients with type 2 diabetes mellitus. BMSCs of normal people and diabetic patients were divided into high sugar group and low sugar group The following indexes were detected: cell proliferation, apoptosis rate, cell adhesion and cell migration ability. Results: The cell proliferation assay showed proliferation ability of BMSCs in low glucose was higher than that in high glucose both in normal and diabetic patients. The apoptosis rate of BMSCs in high group was significantly higher than that in low glucose group in diabetic patients. There was no significant difference of BMSCs apoptosis rate in these groups. There was no statistically significant difference in cell adhesion rate between each group. BMSCs migration ability of normal patients was significantly better than that of the diabetic group. Conclusions:BMSCs cell proliferation ability in diabetic patients was slower than in normal patients. BMSCs cell migration ability in diabetic patients were weaker than in normal patients. However, adipogenic differentiation potential was slightly better than normal patients. High glucose condition could suppress BMSCs cell proliferation and cell migration ability.
引文
[1] L. Guariguata, D. R. Whieing, I. Hambleton, et al. Global estimates of diabetes prevalence for 2013 and projections for2035[J]. Diabetes Res Clin Pract, 2014, 103(2):137-149
[2]中国糖尿病防控专家共识[J].中华预防医学杂志,2017, 51(1):12
[3]吕娇,刘洪臣.糖尿病对种植体骨结合影响的研究进展[J].中华老年口腔医学杂志,2008, 4(6):123-126
[4] Song G, Habibovic P, BaoC. et al. The homing of bone marrow MSCs to non-osseous sites for ectopic bone formation induced by osteoinductive calcium phosphate[J]. Biomaterials, 2013, 34(9):2167-2176
[5] Xin Wang, Yu Wang, Wen long Gou. et al. Role of mesenchymal stem cells in bone regeneration and fracture repair:a review[J]. Int Orthop, 2013, 37(12):2491-2498
[6] Park JC, Kim JC, Kim YT, et al. Acquisition of human alveolar bone-derived stromal cells using minimally irrigated implant osteotomy:in vitro and in vivo evaluations[J]. J Clin Periodontol, 2012, 39:495-505
[7] A Garcia-Hernandez, H Arzate, L Moreno-Fierros, et al.High glucose concentrations alter the biomineralization process in human osteoblastic cells[J]. Bone, 2012, 50:276-288
[8] Dao-Cai Sun, De-Hua Li, Ying-Liang Song, et al. In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics[J]. Braz J Med Biol Res, 2012, 45(6):502-509
[9] Deliloglu-Gurhan SI, Vatansever HS, Ozdal-Kurt F, et al.Characterization of osteoblasts derived from bone marrow stromal cells in a modified cell culture system[J]. Acta Histochem, 2006, 108(1):49-57
[10] Gopalakrishnan V, Vignesh RC, Arunakaran J, et al. Effects of glucose and its modulation by insulin and estradiol on BMSC differentiation into osteoblastic lineages[J]. Biochem Cell Biol, 2006, 84(1):93-101
[11] Webster TJ, Ergun C,Dor emus RH, et al. Enhanced osteoblastlike cell functions on nanophase ceramics[J]. Biomaterils, 2001, 22(11):1327-1333
[12]姜焕焕,刘鑫,赖红昌,等.亲水性纯钛表面对成骨细胞黏附行为的影响[J].中国口腔颌面外科杂志,2011, 9(1):18-22
[13]唐杰,陈龙菊,陈道帮,等.Transwell和Wound healing在细胞迁移中的应用比较[J].中国临床解剖学杂志,2007, 25(6):687-689
[14] J Li, CY Liu, YF Jiang, et al. Proliferation and differentiation of human osteoblasts from a type 2 diabetic patient in vitro[J]. Genetics and Molecular Research, 2015, 14(3):11292-11299
[2]中国糖尿病防控专家共识[J].中华预防医学杂志,2017, 51(1):12
[3]吕娇,刘洪臣.糖尿病对种植体骨结合影响的研究进展[J].中华老年口腔医学杂志,2008, 4(6):123-126
[4] Song G, Habibovic P, BaoC. et al. The homing of bone marrow MSCs to non-osseous sites for ectopic bone formation induced by osteoinductive calcium phosphate[J]. Biomaterials, 2013, 34(9):2167-2176
[5] Xin Wang, Yu Wang, Wen long Gou. et al. Role of mesenchymal stem cells in bone regeneration and fracture repair:a review[J]. Int Orthop, 2013, 37(12):2491-2498
[6] Park JC, Kim JC, Kim YT, et al. Acquisition of human alveolar bone-derived stromal cells using minimally irrigated implant osteotomy:in vitro and in vivo evaluations[J]. J Clin Periodontol, 2012, 39:495-505
[7] A Garcia-Hernandez, H Arzate, L Moreno-Fierros, et al.High glucose concentrations alter the biomineralization process in human osteoblastic cells[J]. Bone, 2012, 50:276-288
[8] Dao-Cai Sun, De-Hua Li, Ying-Liang Song, et al. In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics[J]. Braz J Med Biol Res, 2012, 45(6):502-509
[9] Deliloglu-Gurhan SI, Vatansever HS, Ozdal-Kurt F, et al.Characterization of osteoblasts derived from bone marrow stromal cells in a modified cell culture system[J]. Acta Histochem, 2006, 108(1):49-57
[10] Gopalakrishnan V, Vignesh RC, Arunakaran J, et al. Effects of glucose and its modulation by insulin and estradiol on BMSC differentiation into osteoblastic lineages[J]. Biochem Cell Biol, 2006, 84(1):93-101
[11] Webster TJ, Ergun C,Dor emus RH, et al. Enhanced osteoblastlike cell functions on nanophase ceramics[J]. Biomaterils, 2001, 22(11):1327-1333
[12]姜焕焕,刘鑫,赖红昌,等.亲水性纯钛表面对成骨细胞黏附行为的影响[J].中国口腔颌面外科杂志,2011, 9(1):18-22
[13]唐杰,陈龙菊,陈道帮,等.Transwell和Wound healing在细胞迁移中的应用比较[J].中国临床解剖学杂志,2007, 25(6):687-689
[14] J Li, CY Liu, YF Jiang, et al. Proliferation and differentiation of human osteoblasts from a type 2 diabetic patient in vitro[J]. Genetics and Molecular Research, 2015, 14(3):11292-11299