不同实验条件下食品中半抗原有机磷农药残留的准确测定
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摘要
针对当前食品中有机磷农药残留检测的精度较低,无法精确确定食品中有机磷农药残留,影响食品质量检测效果且检测耗时较长的问题,提出一种通过酶联免疫吸附分析法实现食品中有机磷农药残留检测的方法,首先对实现食品中有机磷农药残留测定的抗原和抗体进行制备,保证所需抗原和抗体符合测定要求,对酶联免疫吸附分析法的实现过程进行分析,确定有机磷农药残留的免疫吸附分析过程,并对酶联免疫吸附分析方法中有机农药农药残留的交叉反应率进行分析,在此基础上,对测定所需的溶剂以及实验仪器进行制备,确定酶联免疫吸附分析法测定食品中有机磷农药残留的实现过程,从而实现酶联免疫吸附分析法测定食品中有机磷农药残留。实验结果表明,所提方法能够准确测定食品中有机磷农药残留,且检测过程较为简单,为该课题向应用领域发展提供理论依据。
Aiming at the problem that the detection precision of organophosphorus pesticide residues in food is low,the organophosphorus pesticide residues in food can not be accurately determined,and the detection effect of food quality is affected and the test takes a long time. In this paper,a method of enzyme-linked immunosorbent assay Organophosphorus pesticide residues in the detection method,first of all to achieve the determination of organophosphorus pesticide residues in food antigens and antibodies prepared to ensure that the required antigens and antibodies meet the assay requirements,the enzymelinked immunosorbent assay analysis of the process to determine the Organophosphorus pesticide residues in the process of immunoadsorption analysis, and analysis of enzyme-linked immunosorbent assay pesticide pesticide residues in the cross-reaction rate analysis,on this basis,the determination of the required solvents and experimental equipment were prepared to determine the enzyme linked Immunoadsorption analysis was used to determine the process of organophosphorus pesticide residues in foodstuffs. In order to achieve enzyme-linked immunosorbent assay Determination of organophosphoruspesticides. The experimental results show that the proposed method can accurately determine organophosphorus pesticide residues in food,and the detection process is relatively simple,providing a theoretical basis for the development of the subject to the application field.
Aiming at the problem that the detection precision of organophosphorus pesticide residues in food is low,the organophosphorus pesticide residues in food can not be accurately determined,and the detection effect of food quality is affected and the test takes a long time. In this paper,a method of enzyme-linked immunosorbent assay Organophosphorus pesticide residues in the detection method,first of all to achieve the determination of organophosphorus pesticide residues in food antigens and antibodies prepared to ensure that the required antigens and antibodies meet the assay requirements,the enzymelinked immunosorbent assay analysis of the process to determine the Organophosphorus pesticide residues in the process of immunoadsorption analysis, and analysis of enzyme-linked immunosorbent assay pesticide pesticide residues in the cross-reaction rate analysis,on this basis,the determination of the required solvents and experimental equipment were prepared to determine the enzyme linked Immunoadsorption analysis was used to determine the process of organophosphorus pesticide residues in foodstuffs. In order to achieve enzyme-linked immunosorbent assay Determination of organophosphoruspesticides. The experimental results show that the proposed method can accurately determine organophosphorus pesticide residues in food,and the detection process is relatively simple,providing a theoretical basis for the development of the subject to the application field.
引文
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[9]白红妍,付明磊,罗琴,等.气-质谱联用法测定罗汉果中有机磷农药残留[J].食品研究与开发,2017,38(17):139-142.
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[2]徐迪,王欢,耿文静,等.气相色谱法测定酸奶中8种有机磷农药的残留[J].中国乳品工业,2015,43(1):36-38.
[3]陈子键,王宇,李美英,等.食品基质有机磷农药残留的前处理新方法研究进展[J].分析测试学报,2015,34(11):1315-1323.
[4]赵海田,生加根,陈弘健. RBAC模型在食品安全溯源平台中的应用[J].电子设计工程,2017,25(7):49-52.
[5]周蓉,曹赵云,赵肖华,等.分散固相萃取-分散液液微萃取/气相色谱-串联质谱法测定蔬菜中19种有机磷农药残留[J].分析测试学报,2017,36(1):67-72.
[6]胡美华,朱惠芳,马丽芳.液下单液滴微萃取/快速气相色谱-质谱联用法测定蔬菜中多种有机磷农药残留[J].分析测试学报,2016,35(5):569-573.
[7]宋丹,李传仁,贾春虹,等.韭菜上五种有机磷农药的残留监测与安全性评价[J].应用昆虫学报,2016,53(6):1255-1260.
[8]郗艳丽,葛红娟,王长文,等.气相色谱法检测蔬菜中7种有机磷农药残留[J].食品研究与开发,2015,36(21):136-138.
[9]白红妍,付明磊,罗琴,等.气-质谱联用法测定罗汉果中有机磷农药残留[J].食品研究与开发,2017,38(17):139-142.
[10]刘凯.蔬菜中有机磷农药多残留的光谱特征检测分析[J].科技通报,2017,33(8):238-242.