单菌株与复合菌对有机物料腐熟性能的影响
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摘要
[目的]研究单菌株和复合菌的腐熟性能。[方法]测定了单菌株和复合菌的拮抗效果、除臭能力、纤维素酶和蛋白酶活性,以及复合菌对有机物料的除臭和腐熟效果。[结果]由TARE1013KC、TARE1003DY、TARE2012SR、TARE2009MQ和TARE2008LS这5种菌株组成的复合菌具有较强的纤维素、蛋白质分解能力及除臭能力,综合性能高于任何单一菌株。复合菌作为腐熟剂进行应用后,从菌数、速效养分、C/N、气味和发酵周期效果来看,对有机物料的腐熟和除臭都起到了显著的促进作用。[结论]复合菌在促进有机物料腐熟及除臭方面效果优于单菌株。
[Objective]To study the maturity performance of single strain and compound bacteria. [Method]The antagonistic effect,deodorization ability,cellulose and protease activity of single strain and compound bacteria,and deodorization and effect of decomposition of compound bacteria on organic materials were determined. [Result] The test result showed that compound bacterium maked up by TARE1013 KC,TARE1003DY,TARE2012 SR,TARE2009MQ and TARE2008 LS had strong comprehensive ability of cellulose,protein decomposition and deodorization ability than any single strain. After compound bacterium as rotten agent was applied in composting,it has played a significant role in promoting the effect of the rotten and deodorization on organic material by indicators of bacterial count,available nutrients,C / N ratio,smell and fermentation cycle. [Conclusion] The compound bacteria in promoting the decomposition of organic materials and deodorization effect was better than that of single strain.
[Objective]To study the maturity performance of single strain and compound bacteria. [Method]The antagonistic effect,deodorization ability,cellulose and protease activity of single strain and compound bacteria,and deodorization and effect of decomposition of compound bacteria on organic materials were determined. [Result] The test result showed that compound bacterium maked up by TARE1013 KC,TARE1003DY,TARE2012 SR,TARE2009MQ and TARE2008 LS had strong comprehensive ability of cellulose,protein decomposition and deodorization ability than any single strain. After compound bacterium as rotten agent was applied in composting,it has played a significant role in promoting the effect of the rotten and deodorization on organic material by indicators of bacterial count,available nutrients,C / N ratio,smell and fermentation cycle. [Conclusion] The compound bacteria in promoting the decomposition of organic materials and deodorization effect was better than that of single strain.
引文
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[10]熊骏生,魏姣皎,陆倩,等.垃圾堆肥过程恶臭污染及其控制技术研究进展[J].杭州师范大学学报(自然科学版),2015,14(4):405-411.
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[14]刘胜洪,周玲艳,刘文,等.生物除臭菌对猪粪堆肥腐熟效果的影响[J].南方农业学报,2014,45(3):425-428.
[15]张云峰.嗜热蛋白酶生产菌的筛选[J].西南师范大学学报(自然科学版),2006,31(2):157-161.
[16]陆文龙,崔广明,陈浩泉,等.微生物除臭剂对污泥和生活垃圾臭气抑制效果的中试研究[J].环境卫生工程,2012,20(2):23-25.
[17]赵晓锋,隋文志.微生物除臭剂研究进展[J].现代化农业,2011(6):26-28.
[18]刘胜洪,程驹,郭长江,等.微生物除臭菌对改善畜禽养殖场环境的应用初探[J].天津农业科学,2011,17(4):25-27.
[19]杨伯杰,张淑娟,林创发,等.污泥堆肥恶臭气体生物处理技术的研究进展[J].广东化工,2014,41(16):124-125.
[20]李南华,胡子全,赵海泉,等.新型生物除臭剂在垃圾渗滤液除臭效果评估[J].广州环境科学,2013,28(1):18-19,30.
[21]崔玉雪,郭广寨,黄皇,等.用于填埋场恶臭气体控制的微生物除臭剂筛选及其除臭机制研究[J].环境污染与防治,2014,36(1):60-63.
[2]柳玲玲,范成五,芶久兰,等.不同腐熟菌剂对鸡粪堆肥效果的影响[J].耕作与栽培,2015(5):18-19.
[3]梁雄.不同秸秆腐熟剂应用效果对比研究[J].安徽农学通报,2011,17(15):127-129.
[4]刘军.产高温蛋白酶高温菌的筛选[J].武汉工业学院学报,2004,23(4):22-23.
[5]翁海波,敬蔚然,杨丹丽,等.产高温碱性蛋白酶菌株筛选及酶学性质研究[J].郑州大学学报(工学版),2010,31(1):70-73.
[6]杨冠东,杜少平,杨础华,等.产碱性蛋白酶的嗜热菌株筛选及发酵条件研究[J].食品与发酵工业,2008,34(10):71-73.
[7]刘月,许修宏,徐杰,等.功能菌剂对堆肥中木质纤维素降解的影响[J].中国土壤与肥料,2014(4):81-86.
[8]赵晓锋,于文清,田艳洪,等.鸡粪除氨菌株的分离、筛选与菌剂配制[J].黑龙江农业科学,2012(6):85-87.
[9]徐大勇,黄为一.接种外源腐熟菌剂对牛粪高温堆肥的影响[J].安徽农业科学,2012,40(13):7759-7762.
[10]熊骏生,魏姣皎,陆倩,等.垃圾堆肥过程恶臭污染及其控制技术研究进展[J].杭州师范大学学报(自然科学版),2015,14(4):405-411.
[11]曾苏,李南华,贺琨,等.垃圾微生物除臭剂的筛选、复配及其培养条件的优化[J].微生物学杂志,2015,35(2):72-77.
[12]席锋,高颖,吴良政,等.生物除臭剂的制备及除臭效果测定[J].贵州畜牧兽医,2011,35(3):1-3.
[13]梁军,罗洪,罗英,等.生物除臭剂对鸡粪除臭处理的研究[J].四川环境,2012,31(S1):13-17.
[14]刘胜洪,周玲艳,刘文,等.生物除臭菌对猪粪堆肥腐熟效果的影响[J].南方农业学报,2014,45(3):425-428.
[15]张云峰.嗜热蛋白酶生产菌的筛选[J].西南师范大学学报(自然科学版),2006,31(2):157-161.
[16]陆文龙,崔广明,陈浩泉,等.微生物除臭剂对污泥和生活垃圾臭气抑制效果的中试研究[J].环境卫生工程,2012,20(2):23-25.
[17]赵晓锋,隋文志.微生物除臭剂研究进展[J].现代化农业,2011(6):26-28.
[18]刘胜洪,程驹,郭长江,等.微生物除臭菌对改善畜禽养殖场环境的应用初探[J].天津农业科学,2011,17(4):25-27.
[19]杨伯杰,张淑娟,林创发,等.污泥堆肥恶臭气体生物处理技术的研究进展[J].广东化工,2014,41(16):124-125.
[20]李南华,胡子全,赵海泉,等.新型生物除臭剂在垃圾渗滤液除臭效果评估[J].广州环境科学,2013,28(1):18-19,30.
[21]崔玉雪,郭广寨,黄皇,等.用于填埋场恶臭气体控制的微生物除臭剂筛选及其除臭机制研究[J].环境污染与防治,2014,36(1):60-63.