高耐镉硫酸盐还原菌群的特性研究
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摘要
从湖南省株洲市镉污染矿区淤泥中富集得到1组硫酸盐还原菌菌群SRB10,研究了该菌群对镉、盐及氧气的耐受性,通过单因子试验考察了温度、pH值和SO_4~(2-)浓度对其生长及硫酸盐还原力的影响,通过正交试验确定了该菌群的最佳工艺条件及影响因子顺序。结果表明,SRB10属于中温耐碱性菌群,能够在镉浓度为175 mg/L或盐度为50 g/L的条件下正常生长,能够耐受一定浓度的氧。单因子试验显示,在培养温度35℃、pH值7.5、SO_4~(2-)浓度1 500 mg/L下,SRB10菌群生长最快,还原硫酸盐的能力最强。正交试验结果表明,对该菌群硫酸盐还原力的影响因素顺序为SO_4~(2-)>p H值>温度>时间,最佳工艺条件为pH值8.0、温度35℃、SO_4~(2-)浓度1 000 mg/L、培养时间40 h,该条件下硫酸盐还原效率可达98.91%。
引文
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[2]Mihcaei W,Anderw J R,Jodi L F,et al.Phylogeny of dissimilatory sufiite reductases supports an early origin of sulfate respiration[J].Bacteriol,1998,180:2975-2982.
[3]Joo J O,Choi J H,Kim I H,et al.Effective bioremediation of cadmium(Ⅱ),nickel(Ⅱ),and chromium(Ⅵ)in a marine environment by using desulfovibrio desulfuricans[J].Biotechnology and Bioprocess Engineering,2015,20(5):937-941.
[4]苏宇,王进,彭书传,等.以稻草和污泥为碳源硫酸盐还原菌处理酸性矿山排水[J].环境科学,2010,31(8):1858-1863.
[5]Kousi P,Remoundaki E,Hatzikioseyian A A,et al.Metal precipitation in an ethanol-fed,fixed-bed sulphate-reducing bioreactor[J].Journal of Hazardous Materials,2011,189(3,SI):677-684.
[6]Omaechevarria J R,Posadas H G,Ferreras J F.First essays for cadmium wastewater elimination by sulphate-reducing bacteria[J].International Journal of Chemical Reactor Engineering,2007,5(1):1-10.
[7]马忠友,邓盾,汪建飞,等.一组混合菌群还原硫酸盐的特性[J].中国农学通报,2013,29(8):184-188.
[8]潘嘉川,曹宏斌,邵宗泽,等.海洋硫酸盐还原菌群处理烟气脱硫废水[J].环境科学,2009,30(2):504-509.
[9]Kolmert A,Wikstrom P,Hallberg K B.A fast and simple turbidimetric method for the determination of sulfate in sulfatereducing bacterial cultures[J].Journal of Microbiological Methods,2000,41(3):179-184.
[10]朱晓丽,张建霞,徐雅雅,等.常温等离子体诱变选育高效耐镉硫酸盐还原菌[J].西北大学学报(自然科学版),2015,45(2):303-307.
[11]周冰玉,吴迎奔,陈薇,等.高耐镉硫酸盐还原菌的筛选及鉴定[J].湖南农业科学,2015(7):91-93.
[12]张小里,陈志昕,刘海洪,等.环境因素对硫酸盐还原菌生长的影响[J].中国腐蚀与防护学报,2000,20(4):224-229.
[13]Bade K,Manz W,Ulrich S.Behavior of sulfate-reducing bacteria under oligotrophic conditions and oxgen stress in particle-free systemsrelated to drinking water[J].FEMS Microbiology Ecolog,2000,32(3):215-223.
[14]山丹.大庆油田注水系统硫酸盐还原菌的分布规律及生态因子研究[D].哈尔滨:哈尔滨工业大学,2005.
[15]刘靖,侯宝利,郑家燊,等.硫酸盐还原菌腐蚀研究进展[J].材料保护,2001,34(8):8-11.
[16]刘安波.硫酸盐还原作用对升流式厌氧污泥床工艺性能影响的研究[D].北京:清华大学,1993.