一株辣椒内生拮抗细菌的筛选及初步鉴定
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摘要
从多年连作重茬的辣椒地采集健康植株,表面消毒后从根系中分离到1株内生拮抗细菌PEB-99,该菌株抗菌谱较广,对辣椒青枯雷尔氏菌、辣椒疫霉菌、辣椒炭疽病菌、辣椒镰刀枯萎病菌都有显著抑制效果,无菌发酵液亦具有抑制细菌和真菌的活性,通过16S r DNA测序对该菌株进行了鉴定,初步认为PEB-99菌株为贝莱斯芽孢杆菌(Bacillus velezensis),该菌能产生铁载体、吲哚-3-乙酸(IAA),还具有溶解有机磷的能力,显示了很好的抗病促生长潜力。
After surface disinfection, an endophytic antagonistic bacteria, named PEB-99 was isolated from the roots of pepper which cultivated in the field with many years continuous cropping, which exhibit strong antagonistic activity against several pathogens of pepper, such as Ralstonia solanacearum, Phytophthora capsici, Colletotrichum capsici, Sclerotium rolfsii, Fusarium oxysporum. The strain was primarily identified as Bacillus velezensis by 16 s r DNA sequencing. The strain still have the ability of production the siderophores and indole-3-acetic acid, and dissolving organic phosphorus, exhibiting well plant-growth-promoting potentiality.
After surface disinfection, an endophytic antagonistic bacteria, named PEB-99 was isolated from the roots of pepper which cultivated in the field with many years continuous cropping, which exhibit strong antagonistic activity against several pathogens of pepper, such as Ralstonia solanacearum, Phytophthora capsici, Colletotrichum capsici, Sclerotium rolfsii, Fusarium oxysporum. The strain was primarily identified as Bacillus velezensis by 16 s r DNA sequencing. The strain still have the ability of production the siderophores and indole-3-acetic acid, and dissolving organic phosphorus, exhibiting well plant-growth-promoting potentiality.
引文
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[2]黄志农,文吉辉,符伟.湖南辣椒生产概况与病虫害绿色防控[J].辣椒杂志,2011,(4):43-47.
[3]曹坳程.毁灭性土传病害综合治理技术体系的构建与创新[J].基层农技推广,2016,(11):108.
[4]曹坳程,刘晓漫,郭美霞,等.作物土传病害的危害及防治技术[J].植物保护,2017,43(2):6-16.
[5]郝楠,仝赞华,邱德文.侧孢短芽孢杆菌A60的筛选及其对辣椒疫霉的室内防效测定[J].生物技术通报,2017,33(9):160-165.
[6]李师默.辣椒和番茄青枯病等土传病害的生物防治研究[D].南京:南京农业大学,2008.
[7]梅新兰,赵青云,谭石勇,等.辣椒疫病拮抗菌株筛选、鉴定及其防效[J].应用生态学报,2010,21(10):2652-2658.
[8]王玲,刘二明,周鑫钰,等.一株辣椒青枯病菌拮抗内生细菌的筛选鉴定与发酵条件优化[J].南方农业学报,2014,45(10):1781-1787.
[9]杨万荣,邢丹,蓬桂华,等.木霉菌生物防治辣椒疫病的研究进展[J].现代农业科技,2015,(19):127-129.
[10]张爱民,邢丹,杨万荣,等.辣椒疫病生物防治技术研究进展[J].长江蔬菜,2013,(8):1-4.
[11]江欢欢,程凯,杨兴明,等.辣椒青枯病拮抗菌的筛选及其生物防治效应[J].土壤学报,2010,47(6):1225-1231.
[12]别小妹,吕凤霞,陆兆新,等.Bacillus subtilis fmb J脂肽类抗菌物质的分离和鉴定[J].生物工程学报,2006,22(4):644-649.
[13]袁珊珊.水稻内生菌Osi Sh-10的分离及其抗稻瘟病与促植物生长的研究[D].长沙:湖南大学,2015.
[14]郭照辉,黄军,魏小武,等.1株广谱拮抗菌的分离鉴定及其抗菌活性成分分析[J].湖南农业大学学报(自科版),2014,40(5):513-518.
[15]李引,虞丽,李辉信,等.一株花生根际促生菌的筛选鉴定及其特性研究[J].生态与农村环境学报,2012,28(4):416-421.
[16]蒙渊.产铁载体和ACC脱氨酶的氢氧化细菌筛选及促生作用研究[D].西安:西北大学,2011.
[17]石晶盈,陈维信,刘爱媛.植物内生菌及其防治植物病害的研究进展[J].生态学报,2006,26(7):2395-2401.
[18]文才艺,吴元华,田秀玲.植物内生菌研究进展及其存在的问题[J].生态学杂志,2004,23(2):86-91.