牙髓干细胞在软组织再生和修复中的研究进展
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摘要
牙髓干细胞是一种来源丰富的成体间充质干细胞,较其他间充质干细胞而言,牙髓干细胞具有其独特优势。近年来,牙髓干细胞不仅仅在硬组织再生与修复研究领域中取得了显著进展,在软组织如神经、血管等的再生和修复领域也日益展现其潜在的作用。但在软组织修复再生方向的应用目前局限于基础研究阶段,本文总结了在软组织再生领域的最新相关研究进展,以期为牙髓干细胞在软组织在再生与修复中的应用提供新思路。
Dental pulp stem cells are a source of abundant adult mesenchymal stem cells, which have their unique advantages over other mesenchymal stem cells. In recent years, dental pulp stem cells have not only made significant progress in the field of hard tissue regeneration and repair research, but also increasingly demonstrated their potential role in the regeneration and repair of soft tissues such as nerves and blood vessels. However, the application of soft tissue repair and regeneration is currently limited to the basic research stage. This paper summarizes the latest research progress in the field of soft tissue regeneration in order to provide new ideas for the application of dental pulp stem cells in soft tissue regeneration and repair
Dental pulp stem cells are a source of abundant adult mesenchymal stem cells, which have their unique advantages over other mesenchymal stem cells. In recent years, dental pulp stem cells have not only made significant progress in the field of hard tissue regeneration and repair research, but also increasingly demonstrated their potential role in the regeneration and repair of soft tissues such as nerves and blood vessels. However, the application of soft tissue repair and regeneration is currently limited to the basic research stage. This paper summarizes the latest research progress in the field of soft tissue regeneration in order to provide new ideas for the application of dental pulp stem cells in soft tissue regeneration and repair
引文
[1] ANITUA E, TROYA M, ZALDUENDO M. Progress in the use of dental pulp stem cells in regenerative medicine[J]. Cytotherapy,2018, 20(4):479-498.
[2] WERLE SB, LINDEMANN D, STEFFENS D, et al. Carious deciduous teeth are a potential source for dental pulp stem cells[J]. Clin Oral Investig, 2016, 20(1):75-81.
[3] HUANG GT, GRONTHOS S, SHI S. Mesenchymal stem cells derived from dental tissues vs. those from other sources:their biology and role in regenerative medicine[J]. J Dent Res, 2009, 88(9):792-806.
[4] ERCAL P, PEKOZER GG, KOSE GT. Dental stem cells in bone tissue engineering:current overview and challenges[J]. Adv Exp Med Biol, 2018, 11(7):113-127.
[5] FOUDAH D, MONFRINI M, DONZELLI E, et al. Expression of neural markers by undifferentiated mesenchymal-like stem cells from different sources[J]. J Immunol Res, 2014, 2014:987678.
[6] LUO L, HE Y, WANG X, et al. Potential roles of dental pulp stem cells in neural regeneration and repair[J]. Stem Cells Int, 2018,2018:1731289.
[7] WANG F, JIA Y, LIU J, et al. Dental pulp stem cells promote regeneration of damaged neuron cells on the cellular model of Alzheimer's disease[J]. Cell Biol Int, 2017, 41(6):639-650.
[8] MITA T, FURUKAWA-HIBI Y, TAKEUCHI H, et al. Conditioned medium from the stem cells of human dental pulp improves cognitive function in a mouse model of Alzheimer’s disease[J]. Behav Brain Res, 2015, 293:189-197.
[9] CHANG CC, CHANG KC, TSAI SJ, et al. Neurogenic differentiation of dental pulp stem cells to neuron-like cells in dopaminergic and motor neuronal inductive media[J]. J Formos Med Assoc, 2014,113(12):956-965.
[10] GANAPATHY K, DATTA I, BHONDE R. Astrocyte-like cells differentiated from dental pulp stem cells protect dopaminergic neurons against 6-hydroxydopamine toxicity[J]. Mol Neurobiol, 2019, 56(6):4395-4413.
[11] GNANASEGARAN N, GOVINDASAMY V, SIMON C, et al. Effect of dental pulp stem cells in MPTP-induced old-aged mice model[J].Eur J Clin Invest, 2017, 47(6):403-414.
[12] FUJII H, MATSUBARA K, SAKAI K, et al. Dopaminergic differentiation of stem cells from human deciduous teeth and their therapeutic benefits for Parkinsonian rats[J]. Brain Res, 2015, 1613:59-72.
[13] MARTENS W, SANEN K, GEORGIOU M, et al. Human dental pulp stem cells can differentiate into Schwann cells and promote and guide neurite outgrowth in an aligned tissue-engineered collagen construct in vitro[J]. FASEB J, 2014, 28(4):1634-1643.
[14] ULLAH I, PARK JM, KANG YH, et al. Transplantation of human dental pulp-derived stem cells or differentiated neuronal cells from human dental pulp-derived stem cells identically enhances regeneration of the injured peripheral nerve[J]. Stem Cells Dev, 2017, 26(17):1247-1257.
[15] ASKARI N, YAGHOOBI MM, SHAMSARA M, et al. Tetracycline-regulated expression of OLIG2 gene in human dental pulp stem cells lead to mouse sciatic nerve regeneration upon transplantation[J]. Neuroscience, 2015, 305:197-208.
[16] WANG P, ZHU S, YUAN C, et al. Shear stress promotes differentiation of stem cells from human exfoliated deciduous teeth into endothelial cells via the downstream pathway of VEGF-Notch signaling[J]. Int J Mol Med, 2018, 42(4):1827-1836.
[17] HILKENS P, FANTON Y, M W, et al. Pro-angiogenic impact of dental stem cells in vitro and in vivo[J]. Stem Cell Res, 2014, 12(3):778-790.
[18] BRONCKAERS A, HILKENS P, FANTON Y, et al. Angiogenic properties of human dental pulp stem cells[J]. PLoS One, 2013, 8(8):e71104.
[19] RATAJCZAK J, BRONCKAERS A, DILLEN Y, et al. The neurovascular properties of dental stem cells and their importance in dental tissue engineering[J]. Stem Cells Int, 2016, 2016:9762871.
[20] GANDIA C, ARMI?AN A, GARCíA-VERDUGOJM, et al. Human dental pulp stem cells improve left ventricular function, induce angiogenesis, and reduce infarct size in rats with acute myocardial infarction[J]. Stem Cells, 2008, 26(3):638-645.
[21] SONG M, LEE JH, BAE J, et al. Human dental pulp stem cells are more effective than human bone marrow-derived mesenchymal stem cells in cerebral ischemic injury[J]. Cell Transplant, 2017, 26(6):1001-1016.
[22] NAKASHIMA M, IOHARA K, SUGIYAMA M. Human dental pulp stem cells with highly angiogenic and neurogenic potential for possible use in pulp regeneration[J]. Cytokine Growth Factor Rev, 2009,20:435-440.
[23] ROOZAFZOON R, LASHAY A, VASEI M, et al. Dental pulp stem cells differentiation into retinal ganglion-like cells in a three dimensional network[J]. Biochem Biophys Res Commun, 2015, 457(2):154-160.
[24] MEAD B, LOGAN A, BERRY M, et al. Paracrine-mediated neuroprotection and neuritogenesis of axotomised retinal ganglion cells by human dental pulp stem cells:comparison with human bone marrow and adipose-derived mesenchymal stem cells[J]. PLoS One, 2014, 9(10):e109305.
[25] MEAD B, LOGAN A, BERRY M, et al. Intravitreally transplanted dental pulp stem cells promote neuroprotection and axon regeneration of retinal ganglion cells after optic nerve injury[J]. Invest Ophthalmol Vis Sci, 2013, 54(12):7544-7556.
[26] MEAD B, HILL LJ, BLANCH RJ, et al. Mesenchymal stromal cell-mediated neuroprotection and functional preservation of retinal ganglion cells in a rodent model of glaucoma[J]. Cytotherapy, 2016,18(4):487-496.
[27] KUSHNEREV E, SHAWCROSS SG, SOTHIRACHAGAN S, et al.Regeneration of corneal epithelium with dental pulp stem cells using a contact lens delivery system[J]. Invest Ophthalmol Vis Sci, 2016,57(13):5192-5199.
[28]ISHKITIEV N, YAEGAKI K, CALENIC B, et al. Deciduous and permanent dental pulp mesenchymal cells acquire hepatic morphologic and functional features in vitro[J]. J Endod, 2010, 36(3):469-474.
[29] MATSUSHITA Y, ISHIGAMI M, MATSUBARA K, et al. Multifaceted therapeutic benefits of factors derived from stem cells from human exfoliated deciduous teeth for acute liver failure in rats[J]. J Tissue Eng Regen Med, 2017, 11(6):1888-1896.
[30] YAMAZA T, ALATAS FS, YUNIARTHA R, et al. In vivo hepatogenic capacity and therapeutic potential of stem cells from human exfoliated deciduous teeth in liver fibrosis in mice[J]. Stem Cell Res Ther,2015, 6:171.
[31] CHO YA, NOH K, JUE SS. Melatonin promotes hepatic differentiation of human dental pulp stem cells:clinical implications for the prevention of liver fibrosis[J]. J Pineal Res, 2015, 58(1):127-135.
[32] KIM HJ, CHO YA, LEE YM, et al. PIN1 suppresses the hepatic differentiation of pulp stem cells via Wnt3a[J]. J Dent Res, 2016, 95(12):1415-1424.
[33] CARNEVALE G, RICCIO M, PISCIOTTA A, et al. In vitro differentiation into insulin-producingβ-cells of stem cells isolated from human amniotic fluid and dental pulp[J]. Dig Liver Dis, 2013, 45(8):669-676.
[34]YAGI MENDOZA H, YOKOYAMA T, TANAKA T, et al. Regeneration of insulin-producing islets from dental pulp stem cells using a3D culture system[J]. Regen Med, 2018, 13(6):673-687.
[35] KIM G, SHIN KH, PAE EK. Zinc up-regulates insulin secretion fromβcell-like cells derived from stem cells from human exfoliated deciduous tooth(SHED)[J]. Int J Mol Sci, 2016, 17(12):2092.
[36] KANAFI MM, RAJESHWARI YB, GUPTA S, et al. Transplantation of islet-like cell clusters derived from human dental pulp stem cells restores normoglycemia in diabetic mice[J]. Cytotherapy, 2013, 15(10):1228-1236.
[37] OMI M, HATA M, NAKAMURA N, et al. Transplantation of dental pulp stem cells improves long-term diabetic polyneuropathy together with improvement of nerve morphometrical evaluation[J]. Stem Cell Res Ther, 2017, 8(1):279.
[2] WERLE SB, LINDEMANN D, STEFFENS D, et al. Carious deciduous teeth are a potential source for dental pulp stem cells[J]. Clin Oral Investig, 2016, 20(1):75-81.
[3] HUANG GT, GRONTHOS S, SHI S. Mesenchymal stem cells derived from dental tissues vs. those from other sources:their biology and role in regenerative medicine[J]. J Dent Res, 2009, 88(9):792-806.
[4] ERCAL P, PEKOZER GG, KOSE GT. Dental stem cells in bone tissue engineering:current overview and challenges[J]. Adv Exp Med Biol, 2018, 11(7):113-127.
[5] FOUDAH D, MONFRINI M, DONZELLI E, et al. Expression of neural markers by undifferentiated mesenchymal-like stem cells from different sources[J]. J Immunol Res, 2014, 2014:987678.
[6] LUO L, HE Y, WANG X, et al. Potential roles of dental pulp stem cells in neural regeneration and repair[J]. Stem Cells Int, 2018,2018:1731289.
[7] WANG F, JIA Y, LIU J, et al. Dental pulp stem cells promote regeneration of damaged neuron cells on the cellular model of Alzheimer's disease[J]. Cell Biol Int, 2017, 41(6):639-650.
[8] MITA T, FURUKAWA-HIBI Y, TAKEUCHI H, et al. Conditioned medium from the stem cells of human dental pulp improves cognitive function in a mouse model of Alzheimer’s disease[J]. Behav Brain Res, 2015, 293:189-197.
[9] CHANG CC, CHANG KC, TSAI SJ, et al. Neurogenic differentiation of dental pulp stem cells to neuron-like cells in dopaminergic and motor neuronal inductive media[J]. J Formos Med Assoc, 2014,113(12):956-965.
[10] GANAPATHY K, DATTA I, BHONDE R. Astrocyte-like cells differentiated from dental pulp stem cells protect dopaminergic neurons against 6-hydroxydopamine toxicity[J]. Mol Neurobiol, 2019, 56(6):4395-4413.
[11] GNANASEGARAN N, GOVINDASAMY V, SIMON C, et al. Effect of dental pulp stem cells in MPTP-induced old-aged mice model[J].Eur J Clin Invest, 2017, 47(6):403-414.
[12] FUJII H, MATSUBARA K, SAKAI K, et al. Dopaminergic differentiation of stem cells from human deciduous teeth and their therapeutic benefits for Parkinsonian rats[J]. Brain Res, 2015, 1613:59-72.
[13] MARTENS W, SANEN K, GEORGIOU M, et al. Human dental pulp stem cells can differentiate into Schwann cells and promote and guide neurite outgrowth in an aligned tissue-engineered collagen construct in vitro[J]. FASEB J, 2014, 28(4):1634-1643.
[14] ULLAH I, PARK JM, KANG YH, et al. Transplantation of human dental pulp-derived stem cells or differentiated neuronal cells from human dental pulp-derived stem cells identically enhances regeneration of the injured peripheral nerve[J]. Stem Cells Dev, 2017, 26(17):1247-1257.
[15] ASKARI N, YAGHOOBI MM, SHAMSARA M, et al. Tetracycline-regulated expression of OLIG2 gene in human dental pulp stem cells lead to mouse sciatic nerve regeneration upon transplantation[J]. Neuroscience, 2015, 305:197-208.
[16] WANG P, ZHU S, YUAN C, et al. Shear stress promotes differentiation of stem cells from human exfoliated deciduous teeth into endothelial cells via the downstream pathway of VEGF-Notch signaling[J]. Int J Mol Med, 2018, 42(4):1827-1836.
[17] HILKENS P, FANTON Y, M W, et al. Pro-angiogenic impact of dental stem cells in vitro and in vivo[J]. Stem Cell Res, 2014, 12(3):778-790.
[18] BRONCKAERS A, HILKENS P, FANTON Y, et al. Angiogenic properties of human dental pulp stem cells[J]. PLoS One, 2013, 8(8):e71104.
[19] RATAJCZAK J, BRONCKAERS A, DILLEN Y, et al. The neurovascular properties of dental stem cells and their importance in dental tissue engineering[J]. Stem Cells Int, 2016, 2016:9762871.
[20] GANDIA C, ARMI?AN A, GARCíA-VERDUGOJM, et al. Human dental pulp stem cells improve left ventricular function, induce angiogenesis, and reduce infarct size in rats with acute myocardial infarction[J]. Stem Cells, 2008, 26(3):638-645.
[21] SONG M, LEE JH, BAE J, et al. Human dental pulp stem cells are more effective than human bone marrow-derived mesenchymal stem cells in cerebral ischemic injury[J]. Cell Transplant, 2017, 26(6):1001-1016.
[22] NAKASHIMA M, IOHARA K, SUGIYAMA M. Human dental pulp stem cells with highly angiogenic and neurogenic potential for possible use in pulp regeneration[J]. Cytokine Growth Factor Rev, 2009,20:435-440.
[23] ROOZAFZOON R, LASHAY A, VASEI M, et al. Dental pulp stem cells differentiation into retinal ganglion-like cells in a three dimensional network[J]. Biochem Biophys Res Commun, 2015, 457(2):154-160.
[24] MEAD B, LOGAN A, BERRY M, et al. Paracrine-mediated neuroprotection and neuritogenesis of axotomised retinal ganglion cells by human dental pulp stem cells:comparison with human bone marrow and adipose-derived mesenchymal stem cells[J]. PLoS One, 2014, 9(10):e109305.
[25] MEAD B, LOGAN A, BERRY M, et al. Intravitreally transplanted dental pulp stem cells promote neuroprotection and axon regeneration of retinal ganglion cells after optic nerve injury[J]. Invest Ophthalmol Vis Sci, 2013, 54(12):7544-7556.
[26] MEAD B, HILL LJ, BLANCH RJ, et al. Mesenchymal stromal cell-mediated neuroprotection and functional preservation of retinal ganglion cells in a rodent model of glaucoma[J]. Cytotherapy, 2016,18(4):487-496.
[27] KUSHNEREV E, SHAWCROSS SG, SOTHIRACHAGAN S, et al.Regeneration of corneal epithelium with dental pulp stem cells using a contact lens delivery system[J]. Invest Ophthalmol Vis Sci, 2016,57(13):5192-5199.
[28]ISHKITIEV N, YAEGAKI K, CALENIC B, et al. Deciduous and permanent dental pulp mesenchymal cells acquire hepatic morphologic and functional features in vitro[J]. J Endod, 2010, 36(3):469-474.
[29] MATSUSHITA Y, ISHIGAMI M, MATSUBARA K, et al. Multifaceted therapeutic benefits of factors derived from stem cells from human exfoliated deciduous teeth for acute liver failure in rats[J]. J Tissue Eng Regen Med, 2017, 11(6):1888-1896.
[30] YAMAZA T, ALATAS FS, YUNIARTHA R, et al. In vivo hepatogenic capacity and therapeutic potential of stem cells from human exfoliated deciduous teeth in liver fibrosis in mice[J]. Stem Cell Res Ther,2015, 6:171.
[31] CHO YA, NOH K, JUE SS. Melatonin promotes hepatic differentiation of human dental pulp stem cells:clinical implications for the prevention of liver fibrosis[J]. J Pineal Res, 2015, 58(1):127-135.
[32] KIM HJ, CHO YA, LEE YM, et al. PIN1 suppresses the hepatic differentiation of pulp stem cells via Wnt3a[J]. J Dent Res, 2016, 95(12):1415-1424.
[33] CARNEVALE G, RICCIO M, PISCIOTTA A, et al. In vitro differentiation into insulin-producingβ-cells of stem cells isolated from human amniotic fluid and dental pulp[J]. Dig Liver Dis, 2013, 45(8):669-676.
[34]YAGI MENDOZA H, YOKOYAMA T, TANAKA T, et al. Regeneration of insulin-producing islets from dental pulp stem cells using a3D culture system[J]. Regen Med, 2018, 13(6):673-687.
[35] KIM G, SHIN KH, PAE EK. Zinc up-regulates insulin secretion fromβcell-like cells derived from stem cells from human exfoliated deciduous tooth(SHED)[J]. Int J Mol Sci, 2016, 17(12):2092.
[36] KANAFI MM, RAJESHWARI YB, GUPTA S, et al. Transplantation of islet-like cell clusters derived from human dental pulp stem cells restores normoglycemia in diabetic mice[J]. Cytotherapy, 2013, 15(10):1228-1236.
[37] OMI M, HATA M, NAKAMURA N, et al. Transplantation of dental pulp stem cells improves long-term diabetic polyneuropathy together with improvement of nerve morphometrical evaluation[J]. Stem Cell Res Ther, 2017, 8(1):279.