复方增氧方耐缺氧作用及初步血清药物化学研究
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摘要
目的探讨复方增氧方提高鼠缺氧耐受力作用及安全性,并初步探讨其入血成分。方法通过常压耐缺氧实验、亚硝酸钠中毒存活实验、急性脑缺血试验记录小鼠存活时间、SOD活性和MDA含量测定,通过小鼠急性毒性试验记录小鼠状况及血清药物化学检测测定大鼠入血的原型成分。结果小鼠耐缺氧实验表明复方增氧方可以提高小鼠缺氧及中毒情况下存活时间,急性毒性试验表明复方增氧方无毒性,通过血清药物化学检测判断出9个入血的原型成分。结论复方增氧方安全无毒,可以显著增强小鼠存活时间,增加SOD活性,MDA含量显著降低,初步探知了该复方提取物在大鼠体内入血的原型成分,为后续研究提供依据。
Objective To investigate the ef fect and safety of Fu Fang Zeng Yang Fang on hypoxia tolerance in rats and to investigate the blood infusions. Methods The survival time, SOD activity and MDA content of mice were recorded by atmospheric pressure test, sodium nitrite poisoning survival test, acute cerebral ischemia test, mouse acute toxicity test, and serum drug chemistry test. Results The hypoxia tolerance test in mice showed that the Fu Fang Zeng Yang Fang can increase the survival time of mice under hypoxia and poisoning. The acute toxicity test showed that the Fu Fang Zeng Yang Fang was non-toxic, and the prototype components of 9 blood samples were determined by serum chemistry test. Conclusion Fu Fang Zeng Yang Fang is safe and non-toxic, can significantly enhance the survival time of mice, increase the SOD activity, significantly reduce the MDA content, and preliminarily detect its extracts in rats,providing evidence for subsequent studies.
Objective To investigate the ef fect and safety of Fu Fang Zeng Yang Fang on hypoxia tolerance in rats and to investigate the blood infusions. Methods The survival time, SOD activity and MDA content of mice were recorded by atmospheric pressure test, sodium nitrite poisoning survival test, acute cerebral ischemia test, mouse acute toxicity test, and serum drug chemistry test. Results The hypoxia tolerance test in mice showed that the Fu Fang Zeng Yang Fang can increase the survival time of mice under hypoxia and poisoning. The acute toxicity test showed that the Fu Fang Zeng Yang Fang was non-toxic, and the prototype components of 9 blood samples were determined by serum chemistry test. Conclusion Fu Fang Zeng Yang Fang is safe and non-toxic, can significantly enhance the survival time of mice, increase the SOD activity, significantly reduce the MDA content, and preliminarily detect its extracts in rats,providing evidence for subsequent studies.
引文
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[5]陈俊莉,蔡浩斌,罗思,等.阿魏酸对不同时间点糖氧剥夺诱导脑微血管内皮细胞氧化损伤的保护作用[J].中药新药与临床药理, 2015, 26(5):622-626.
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[7]唐峥,尹然,毕开顺,等.栀子大黄汤血清药物化学的初步研究[J].中草药, 2014, 45(23):3377-3382.
[8]成新平,苏韫,颜春鲁,等.黄芪百合颗粒对常压缺氧小鼠脑组织的保护作用[J].解放军药学学报, 2017, 33(5):425-427, 431.
[9]陈广通,杨敏,果德安.人参皂苷Re在大鼠体内的代谢研究[J].中国中药杂志, 2009, 34(12):1540-1543.
[2]李芸达,颜祖弟,黄涛,等.羌活不同提取物对小鼠脑缺血缺氧的保护作用[J].中国医院药学杂志, 2015, 35(4):296-299.
[3]许锦珍,刘文君,付玉梅,等.参灵草口服液对小鼠耐缺氧实验研究[J].江西中医药, 2017, 48(4):59-60.
[4]马艳,汪宇,康万军,等.蛹虫草口服液对小鼠耐缺氧和抗疲劳作用[J].中国药师, 2012, 15(10):1375-1377.
[5]陈俊莉,蔡浩斌,罗思,等.阿魏酸对不同时间点糖氧剥夺诱导脑微血管内皮细胞氧化损伤的保护作用[J].中药新药与临床药理, 2015, 26(5):622-626.
[6]金亚香,张研,刘天戟.茶树菇提取物抗疲劳及耐缺氧作用研究[J].时珍国医国药, 2016, 27(3):608-611.
[7]唐峥,尹然,毕开顺,等.栀子大黄汤血清药物化学的初步研究[J].中草药, 2014, 45(23):3377-3382.
[8]成新平,苏韫,颜春鲁,等.黄芪百合颗粒对常压缺氧小鼠脑组织的保护作用[J].解放军药学学报, 2017, 33(5):425-427, 431.
[9]陈广通,杨敏,果德安.人参皂苷Re在大鼠体内的代谢研究[J].中国中药杂志, 2009, 34(12):1540-1543.