响应面法优化二色补血草多糖的乙酰化工艺
|
摘要
采用响应面试验研究合成乙酰化二色补血草多糖的最优工艺条件,以乙酰化多糖取代度为评价指标,考察反应时间、乙酸酐与多糖的物质的量比和反应温度对乙酰化多糖取代度的影响。结果表明,通过响应面试验得到的最优工艺条件为:反应时间2.6 h、乙酸酐与多糖的物质的量比3.3:1、反应温度65℃。在此条件下,二色补血草乙酰化多糖取代度为0.409。初步的抗氧化性实验表明,乙酰化后的二色补血草多糖的抗氧化性能有了明显提高。
In this study, response surface methodology was employed to optimize the acetylation conditions of polysaccharides from Limonium bicolor Kunze. The degree of substitution(Ds) of acetylated polysaccharides was investigated as a function of reaction time, molar ratio of acetic anhydride to polysaccharides, and reaction temperature. The optimal conditions for acetylating polysaccharides from Limonium bicolor Kunze were found to be reaction at 65 ℃ for 2.6 h with an acetic anhydride-to-polysaccharide ratio of 3.3:1. The maximum DS of 0.409 was obtained under the optimized reaction conditions. Antioxidant assays demonstrated that compared with Limonium bicolor Kunze polysaccharides, the antioxidant capacity of acetylated derivatives was significantly improved.
In this study, response surface methodology was employed to optimize the acetylation conditions of polysaccharides from Limonium bicolor Kunze. The degree of substitution(Ds) of acetylated polysaccharides was investigated as a function of reaction time, molar ratio of acetic anhydride to polysaccharides, and reaction temperature. The optimal conditions for acetylating polysaccharides from Limonium bicolor Kunze were found to be reaction at 65 ℃ for 2.6 h with an acetic anhydride-to-polysaccharide ratio of 3.3:1. The maximum DS of 0.409 was obtained under the optimized reaction conditions. Antioxidant assays demonstrated that compared with Limonium bicolor Kunze polysaccharides, the antioxidant capacity of acetylated derivatives was significantly improved.
引文
[1]汤新慧,沈敏.补血草属植物化学成分及药理作用研究进展[J].时珍国医国药,2007,18(8):1874-1876.
[2]魏友霞,王军宪,姚鸿萍.补血草属植物化学成分和药理作用研究进展[J].西北药学杂志,2007,22(4):222-225.
[3]LIN L C,KUOY C,CHOU C J.Anti-herpes simplex virus type-1flavonoids and a new flavanone from the root of Limonium sinese[J].Planta Medica,2000,66(3):333-336.
[4]BASHIR A K,ABDALLA A A,WASFI I A.Flavonoids of Limonium axillare[J].International Journal of Pharmacognosy,1994,32(4):366-372.
[5]魏友霞,王军宪,姚鸿萍.二色补血草挥发油成分气相-质谱联用分析[J].中国现代应用药学杂志,2007,24(5):398-401.
[6]吴冬青,安红钢,林敏,等.二色补血草不同部位总黄酮含量的测定[J].光谱实验室,2006,23(1):127-129.
[7]魏有霞,王军宪.二色补血草地下部分化学成分研究[J].中草药,2006,29(11):1182-1184.
[8]李莉,杨汉民.二色补血草临床止血206例疗效观察[J].陕西中医,2001,22(11):649.
[9]张连茹,陈喀林,李妮,等.二色补血草多糖的结构表征及其对HeLa细胞的抑制作用[J].高等学校化学学报,2004,25(11):2034-2037.
[10]王晶,张全斌,张忠山,等.乙酰化海带褐藻多糖硫酸酯的制备及其抗氧化活性研究[J].中国海洋药物杂志,2008,27(1):50-54.
[11]梁忠岩,苗春艳,张翼伸,等.化学修饰对斜顶菌多糖抑菌活性影响的研究[J].中国药学杂志,1996,30(10):613-615.
[12]QI Huimin,ZHANG Quanbin,ZHAO Tingting,et al.In vitro antioxidant activity of acetylated and benzoylated derivatives of polysaccharide extracted from Ulva pertusa(Chlorophyta)[J].Bioorganic and Medicinal Chemistry Letters,2006,16:2441-2445.
[13]QI Huimin,ZHAO Tingting,ZHANG Quanbin,et al.Antioxidant activity of different molecular weight sulfated polysaccharides from Ulva pertusa Kjellm(Chlorophyta)[J].Journal of Applied Phycology,2005,17:527-534.
[14]朱玉婷,谭姚,莫开菊.硫酸酯化修饰葛仙米多糖工艺研究[J].食品科学,2011,32(24):46-49.
[15]田龙,王庆林,阚云超,等.正交试验优化猕猴桃果水溶性多糖硫酸酯化条件的研究[J].食品科学,2007,28(5):103-106.
[16]梁进,张剑韵,崔莹莹,等.茶多糖的化学修饰及体外抗凝血作用研究[J].茶叶科学,2008,28(3):166-171.
[17]李书福,周革非,王长海.乙酰化修饰的角叉菜λ-卡拉胶的免疫和抗氧化活性研究[J].食品科技,2010,35(5):124-128.
[18]黄丹菲,聂少平,江乐明,等.乙酰化大粒车前子多糖的制备及其活性初探[J].食品科学,2013,34(22):1-4.
[19]殷殷,张燕,彭芳,等.紫外分光光度法测定魔芋多糖中乙酰取代度[J].药物分析杂志,2010,30(5):919-921.
[20]赵鹏.款冬花多糖提取纯化工艺研究及结构鉴定[D].西安:西北大学,2010.
[21]赵鹏,李稳宏,朱骤海,等.超声提取款冬花多糖的响应面法工艺优化研究[J].精细化工,2009,26(6):546-549.
[22]王俊杰,赵燕,涂勇刚,等.响应面法优化受精蛋蛋清制备抗氧化肽酶解工艺[J].食品科学,2014,35(9):187-191.
[23]邱远,芮昕,谢翌冬,等.响应面法优化超声提取葛根素工艺[J].食品科学,2014,35(6):1-5.
[24]张辉,张娜娜,马丽,等.响应面法优化大麦苗叶绿素提取工艺[J].食品科学,2014,35(2):75-80.
[25]周林,郭祀远,郑必胜,等.裂褶多糖的乙酰化及光谱分析[J].华南理工大学学报:自然科学版,2006,34(12):88-91.
[2]魏友霞,王军宪,姚鸿萍.补血草属植物化学成分和药理作用研究进展[J].西北药学杂志,2007,22(4):222-225.
[3]LIN L C,KUOY C,CHOU C J.Anti-herpes simplex virus type-1flavonoids and a new flavanone from the root of Limonium sinese[J].Planta Medica,2000,66(3):333-336.
[4]BASHIR A K,ABDALLA A A,WASFI I A.Flavonoids of Limonium axillare[J].International Journal of Pharmacognosy,1994,32(4):366-372.
[5]魏友霞,王军宪,姚鸿萍.二色补血草挥发油成分气相-质谱联用分析[J].中国现代应用药学杂志,2007,24(5):398-401.
[6]吴冬青,安红钢,林敏,等.二色补血草不同部位总黄酮含量的测定[J].光谱实验室,2006,23(1):127-129.
[7]魏有霞,王军宪.二色补血草地下部分化学成分研究[J].中草药,2006,29(11):1182-1184.
[8]李莉,杨汉民.二色补血草临床止血206例疗效观察[J].陕西中医,2001,22(11):649.
[9]张连茹,陈喀林,李妮,等.二色补血草多糖的结构表征及其对HeLa细胞的抑制作用[J].高等学校化学学报,2004,25(11):2034-2037.
[10]王晶,张全斌,张忠山,等.乙酰化海带褐藻多糖硫酸酯的制备及其抗氧化活性研究[J].中国海洋药物杂志,2008,27(1):50-54.
[11]梁忠岩,苗春艳,张翼伸,等.化学修饰对斜顶菌多糖抑菌活性影响的研究[J].中国药学杂志,1996,30(10):613-615.
[12]QI Huimin,ZHANG Quanbin,ZHAO Tingting,et al.In vitro antioxidant activity of acetylated and benzoylated derivatives of polysaccharide extracted from Ulva pertusa(Chlorophyta)[J].Bioorganic and Medicinal Chemistry Letters,2006,16:2441-2445.
[13]QI Huimin,ZHAO Tingting,ZHANG Quanbin,et al.Antioxidant activity of different molecular weight sulfated polysaccharides from Ulva pertusa Kjellm(Chlorophyta)[J].Journal of Applied Phycology,2005,17:527-534.
[14]朱玉婷,谭姚,莫开菊.硫酸酯化修饰葛仙米多糖工艺研究[J].食品科学,2011,32(24):46-49.
[15]田龙,王庆林,阚云超,等.正交试验优化猕猴桃果水溶性多糖硫酸酯化条件的研究[J].食品科学,2007,28(5):103-106.
[16]梁进,张剑韵,崔莹莹,等.茶多糖的化学修饰及体外抗凝血作用研究[J].茶叶科学,2008,28(3):166-171.
[17]李书福,周革非,王长海.乙酰化修饰的角叉菜λ-卡拉胶的免疫和抗氧化活性研究[J].食品科技,2010,35(5):124-128.
[18]黄丹菲,聂少平,江乐明,等.乙酰化大粒车前子多糖的制备及其活性初探[J].食品科学,2013,34(22):1-4.
[19]殷殷,张燕,彭芳,等.紫外分光光度法测定魔芋多糖中乙酰取代度[J].药物分析杂志,2010,30(5):919-921.
[20]赵鹏.款冬花多糖提取纯化工艺研究及结构鉴定[D].西安:西北大学,2010.
[21]赵鹏,李稳宏,朱骤海,等.超声提取款冬花多糖的响应面法工艺优化研究[J].精细化工,2009,26(6):546-549.
[22]王俊杰,赵燕,涂勇刚,等.响应面法优化受精蛋蛋清制备抗氧化肽酶解工艺[J].食品科学,2014,35(9):187-191.
[23]邱远,芮昕,谢翌冬,等.响应面法优化超声提取葛根素工艺[J].食品科学,2014,35(6):1-5.
[24]张辉,张娜娜,马丽,等.响应面法优化大麦苗叶绿素提取工艺[J].食品科学,2014,35(2):75-80.
[25]周林,郭祀远,郑必胜,等.裂褶多糖的乙酰化及光谱分析[J].华南理工大学学报:自然科学版,2006,34(12):88-91.