亚麻原麻及其粗纱生物酶处理工艺条件的优化
|
摘要
以亚麻打成麻及其粗纱为研究对象,以质量损失率、强度和细度(分裂度)作为评定标准,对生物酶处理工艺进行了研究。结果表明:在p H值为4的柠檬酸-柠檬酸钠缓冲溶液中,在40℃下,以180 r/min振荡处理4 h,果胶酶、木聚糖酶和甘露聚糖混合处理后的打成麻及粗纱的分裂度和强度最优,分裂度和细度分别为663 Nm和1.03 tex,强度分别为17.07、34.02 c N/tex,纤维强度均比化学方法处理后的高。采用扫描电镜、红外光谱对纤维上胶质的去除情况进行了分析,结果显示:酶处理后的麻纤维表面更加光滑;胶质含量下降。
This study aims at scotched flax and flax roving,besides,the weight loss,strength and the degree of fiber splitting as a measure of the standard for different enzyme treatment process. Under the conditions of the enzyme treatment: p H 4. 0,0. 1 mol/L of citric acid-sodium citrate buffer,40 ℃ and180 r/min with shock treatment,4 h. The results suggested that the tenacity and fineness of fiber after using mixing enzymes are 663 Nm and 1. 03 tex,respectively,and the strength is 17. 07 c N/tex and34. 02 c N/tex,respectively,which are higher than the conventional scouring process. In addition,scanning electron microscopy( SEM) and FT-IR spectroscopy were used to evaluate the removal of gummy materials on the fiber bundles. SEM results showed that the surface of hemp fiber is much smoother by the enzyme treatment; and the FT-IR spectroscopic results suggested the gum decreased.
This study aims at scotched flax and flax roving,besides,the weight loss,strength and the degree of fiber splitting as a measure of the standard for different enzyme treatment process. Under the conditions of the enzyme treatment: p H 4. 0,0. 1 mol/L of citric acid-sodium citrate buffer,40 ℃ and180 r/min with shock treatment,4 h. The results suggested that the tenacity and fineness of fiber after using mixing enzymes are 663 Nm and 1. 03 tex,respectively,and the strength is 17. 07 c N/tex and34. 02 c N/tex,respectively,which are higher than the conventional scouring process. In addition,scanning electron microscopy( SEM) and FT-IR spectroscopy were used to evaluate the removal of gummy materials on the fiber bundles. SEM results showed that the surface of hemp fiber is much smoother by the enzyme treatment; and the FT-IR spectroscopic results suggested the gum decreased.
引文
[1]丁若垚.亚麻粗纱脱胶微生物的选育与应用[D].上海:东华大学,2013:3-5.DING Ruoyao.Selection and application of microorganism for scouring of flax roving[D].Shanghai:Donghua University,2013:3-5.
[2]郑磊,丁若垚,郁崇文.脱胶细菌在亚麻粗纱煮练中的初步应用[J].纺织学报,2012,33(8):66-70.ZHENG Lei,DING Ruoyao,YU Chongwen.Preliminary application of degumming bacterium in scouring of flax roves[J].Journal of Textile Research,2012,33(8):66-70.
[3]彭源德,刘正初,金关荣,等.亚麻快速生物脱胶技术工厂化生产研究[J].中国农业科学,2005,38(4):849-853.PENG Yuande,LIU Zhengchu,JIN Guanrong,et al.Study on technique of fast microbial retting of flax[J].Scientia Agricultura Sinica,2005,38(4):849-853.
[4]BASUS,SAHAMN.Large-scale degumming of ramie fibre using a newly isolated Bacillus pumilus DKSl with high pectatelyase activity[J].Journal of lndustrial Microbiology&Biotechnology,2009,36(2):239-245.
[5]彭源德,唐守伟,杨喜爱,等.大麻快速脱胶菌株的选育与脱胶性能鉴定[J].纺织学报,2007,28(12):66-68.PENG Yuande,TANG Shouwei,YANG Xiai,et al.Breeding of a fast retting bacterium and appraisal of retting function in hemp[J].Journal of Textile Research,2007,28(12):66-68.
[6]王清吉,尹西竹,刘永举,等.DNS法测定饲用木聚糖活力[J].兽药与饲料添加剂,2000(5):10.WANG Qingji,YIN Xizhu,LIU Yongju,et al.DNS assay feeding xylanase activity[J].Veterinary Pharmaceuticals&Feed Additives,2000(5):10.
[7]董政娥,丁若垚,郑磊,等.亚麻粗纱细菌煮练工艺优化探讨[J].毛纺科技,2013(3):42-46.DONG Zheng`e,DING Ruoyao,ZHENG Lei,et al.Study on optimizing bacteria scouring of flax roving[J].Wool Textile Journal,2013(3):42-46.
[8]刘幸乐,姚继明,吴远明.酸的种类、p H和温度对酸性纤维素酶活力的影响[J].印染助剂,2012(12):11-14.LIU Xingle,YAO Jiming,WU Yuanming.Effects of acids,p H and temperature on acid cellulase activity[J].Textile Auxiliaries,2012(12):11-14.
[9]魏有霞,韩增祥.酶制剂活性的测定及稳定性的研究[J].饲料工业,2006(24):46-48.WEI Youxia,HAN Zengxiang.The estimation of active and study of stability on enzyme preparation[J].Feed Industry,2006(24):46-48.
[10]杨辉,张娟,王旭.果胶酶活力测定中酶活力与稀释倍数的研究[J].食品研究与开发,2006(9):96-98.YANG Hui,ZHANG Juan,WANG Xu.Study on the relationship between the activity of rectinase and the dilution ratio in pectinase assays[J].Food Research and Developent,2006(9):96-98.
[11]李长影,孔雯,王家昕,等.β-甘露聚糖酶产生菌的分离鉴定和酶学性质[J].华中农业大学学报,2011(2):138-142.LI Changying,KONG Wen,WANG Jiaxin,et al.Isolation identification and enzymatic characterization ofβ-mannanase producing strain[J].Journal of Huazhong Agricultural University,2011(2):138-142.
[12]聂国兴,李春喜,张建新,等.底物浓度、DNS量对木聚糖酶活性测定结果的影响[J].饲料工业,2002(11):24-25.NIE Guoxing,LI Chunxi,ZHANG Jianxin,et al.The effects of substrate concentration and DNS on the activity of xylanase[J].Feed Industry,2002(11):24-25.
[2]郑磊,丁若垚,郁崇文.脱胶细菌在亚麻粗纱煮练中的初步应用[J].纺织学报,2012,33(8):66-70.ZHENG Lei,DING Ruoyao,YU Chongwen.Preliminary application of degumming bacterium in scouring of flax roves[J].Journal of Textile Research,2012,33(8):66-70.
[3]彭源德,刘正初,金关荣,等.亚麻快速生物脱胶技术工厂化生产研究[J].中国农业科学,2005,38(4):849-853.PENG Yuande,LIU Zhengchu,JIN Guanrong,et al.Study on technique of fast microbial retting of flax[J].Scientia Agricultura Sinica,2005,38(4):849-853.
[4]BASUS,SAHAMN.Large-scale degumming of ramie fibre using a newly isolated Bacillus pumilus DKSl with high pectatelyase activity[J].Journal of lndustrial Microbiology&Biotechnology,2009,36(2):239-245.
[5]彭源德,唐守伟,杨喜爱,等.大麻快速脱胶菌株的选育与脱胶性能鉴定[J].纺织学报,2007,28(12):66-68.PENG Yuande,TANG Shouwei,YANG Xiai,et al.Breeding of a fast retting bacterium and appraisal of retting function in hemp[J].Journal of Textile Research,2007,28(12):66-68.
[6]王清吉,尹西竹,刘永举,等.DNS法测定饲用木聚糖活力[J].兽药与饲料添加剂,2000(5):10.WANG Qingji,YIN Xizhu,LIU Yongju,et al.DNS assay feeding xylanase activity[J].Veterinary Pharmaceuticals&Feed Additives,2000(5):10.
[7]董政娥,丁若垚,郑磊,等.亚麻粗纱细菌煮练工艺优化探讨[J].毛纺科技,2013(3):42-46.DONG Zheng`e,DING Ruoyao,ZHENG Lei,et al.Study on optimizing bacteria scouring of flax roving[J].Wool Textile Journal,2013(3):42-46.
[8]刘幸乐,姚继明,吴远明.酸的种类、p H和温度对酸性纤维素酶活力的影响[J].印染助剂,2012(12):11-14.LIU Xingle,YAO Jiming,WU Yuanming.Effects of acids,p H and temperature on acid cellulase activity[J].Textile Auxiliaries,2012(12):11-14.
[9]魏有霞,韩增祥.酶制剂活性的测定及稳定性的研究[J].饲料工业,2006(24):46-48.WEI Youxia,HAN Zengxiang.The estimation of active and study of stability on enzyme preparation[J].Feed Industry,2006(24):46-48.
[10]杨辉,张娟,王旭.果胶酶活力测定中酶活力与稀释倍数的研究[J].食品研究与开发,2006(9):96-98.YANG Hui,ZHANG Juan,WANG Xu.Study on the relationship between the activity of rectinase and the dilution ratio in pectinase assays[J].Food Research and Developent,2006(9):96-98.
[11]李长影,孔雯,王家昕,等.β-甘露聚糖酶产生菌的分离鉴定和酶学性质[J].华中农业大学学报,2011(2):138-142.LI Changying,KONG Wen,WANG Jiaxin,et al.Isolation identification and enzymatic characterization ofβ-mannanase producing strain[J].Journal of Huazhong Agricultural University,2011(2):138-142.
[12]聂国兴,李春喜,张建新,等.底物浓度、DNS量对木聚糖酶活性测定结果的影响[J].饲料工业,2002(11):24-25.NIE Guoxing,LI Chunxi,ZHANG Jianxin,et al.The effects of substrate concentration and DNS on the activity of xylanase[J].Feed Industry,2002(11):24-25.