补气药人参、黄芪防治心脑疾病的网络药理学研究
|
摘要
基于网络药理学研究补气中药人参、黄芪的主要活性成分、靶点及作用于心脑疾病的分子机制。通过中药整合药理学平台(TCMIP),筛选出人参和黄芪的成分、靶标信息,进行相关的GO基因功能和KEGG通路富集分析,构建核心靶标网络图和"中药-成分-关键药靶-通路"图。从人参、黄芪药对中筛选得到160个药物间共同靶标,463条药物-疾病核心靶标信息,其中62条共同靶标、131条潜在药物靶标、25条已知疾病靶标,主要涉及在线粒体能量产生、转化及氧化代谢等多条生物学过程信号通路。阐释了人参、黄芪功效及应用的异同点,并从成分-靶点-疾病角度初步揭示了人参、黄芪治疗心脑疾病具有补气功效共同作用机制,为深入研究中药功效的作用机制提供了示范和参考。
To study of the main active components,targetsof Ginseng and Astragalusand theirmolecular mechanisms of cardiac or cerebral diseases by networkpharmacology.Based on the Integrative Pharmacology of Traditional Chinese Medicine(TCMIP),we have screened the components and target information of Ginseng and Astragalus,and carried out the related GO gene function and KEGG pathway enrichment analysis,andconstructed the core target network diagram and the " Traditional Chinese Medicine-ingredient-key drug target-pathway" network diagram.The160 common target of drugs,463 drug-diseasecore target informationof are screened from Ginseng and Astragalus hebral disease.Among the 463 drug-diseasecore target information,the 62 common targets,131 potential drug targets and 25 known disease targets are mainly involved in multiple biological processes,such as mitochondrial energy production,transformation and oxidative metabolism.This study explains the similarities and differences of the effect and application onGinseng and Astragalus,which preliminarily revealthe mechanism of combinedaction that Ginseng and Astragalus in the treatment of cardio-cerebral diseases have the effect of Tonifying Qi,and it also provides a demonstration and reference for the in-depth study of the mechanism of the effect of traditional Chinese medicine.
To study of the main active components,targetsof Ginseng and Astragalusand theirmolecular mechanisms of cardiac or cerebral diseases by networkpharmacology.Based on the Integrative Pharmacology of Traditional Chinese Medicine(TCMIP),we have screened the components and target information of Ginseng and Astragalus,and carried out the related GO gene function and KEGG pathway enrichment analysis,andconstructed the core target network diagram and the " Traditional Chinese Medicine-ingredient-key drug target-pathway" network diagram.The160 common target of drugs,463 drug-diseasecore target informationof are screened from Ginseng and Astragalus hebral disease.Among the 463 drug-diseasecore target information,the 62 common targets,131 potential drug targets and 25 known disease targets are mainly involved in multiple biological processes,such as mitochondrial energy production,transformation and oxidative metabolism.This study explains the similarities and differences of the effect and application onGinseng and Astragalus,which preliminarily revealthe mechanism of combinedaction that Ginseng and Astragalus in the treatment of cardio-cerebral diseases have the effect of Tonifying Qi,and it also provides a demonstration and reference for the in-depth study of the mechanism of the effect of traditional Chinese medicine.
引文
[1]刘月,初杰.人参黄芪的性味与配伍对方剂构成的影响[J].辽宁中医药大学学报,2008,10(5):5-6.
[2]贾蔷,唐仕欢,石作荣,等.从中医气血理论探析“脑心同治”[J].世界中医药,2014(10):1293-1295.
[3]王冬菊.心脑血管疾病流行概况及主要影响因素[J].预防医学论坛,2016,22(01):71-75.
[4]郭优勤,魏桂林,钟秋明,等.浅谈人参的临床应用[J].中国现代药物应用,2011,05(7):128-129.
[5]刘会艳.黄芪及其制剂的药理作用和临床应用[J].内蒙古中医药,2012,31(22):44-45.
[6]许海玉,刘振明,付岩,等.中药整合药理学计算平台的开发与应用[J].中国中药杂志,2017,42(18):3633-3638.
[7]Lipinski C A,Lombardo F,Dominy B W,et al.Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings 1[J].Advanced Drug Delivery Reviews,2001,46(1-3):3-26.
[8]Gaignard Pauline,Liere Philippe,Thérond Patrice,et al.Role of Sex Hormones on Brain Mitochondrial Function,with Special Reference to Aging and Neurodegenerative Diseases[J].Frontiers in Aging Neuroscience,2017,9:406.
[9]宋齐.人参化学成分和药理作用研究进展[J].人参研究,2017,29(2):47-54.
[10]曹玉冰.黄芪甲苷的药理作用及其机制的研究进展[J].现代药物与临床,2017,32(05):954-960.
[11]Fernandes-Rosa F L,Boulkroun S,Zennaro M C.Somatic and inherited mutations in primary aldosteronism[J].Journal of Molecular Endocrinology,2017,59(1):47-63.
[12]Hu H J,Song M.Disrupted Ionic Homeostasis in Ischemic Stroke and New Therapeutic Targets.[J].J Stroke Cerebrovasc Dis,2017,26(12):2706-2719.
[13]Nd D G,Vega R B,Kelly D P.Mitochondrial biogenesis and dynamics in the developing and diseased heart.[J].Genes&Development,2015,29(19):1981-1991.
[14]Fan X,Xie J,Tian J.Reducing Cardiac Fibrosis:Na/K-ATPase Signaling Complex as a Novel Target[J].Cardiovasc Pharm Open Access,2017,6(1):1-12.
[15]Heslegrave A J,Hussain K.Novel insights into fatty acid oxidation,amino acid metabolism,and insulin secretion from studying patients with loss of function mutations in 3-hydroxyacyl-CoA dehydrogenase[J].Journal of Clinical Endocrinology&Metabolism,2013,98(2):496.
[16]Malik B,Fernandes C,Killick R,et al.Oligomeric amyloid-βpeptide affects the expression of genes involved in steroid and lipid metabolism in primary neurons.[J].Neurochemistry International,2012,61(3):321-333.
[17]Chen J,Young M E,Chatham J C,et al.TXNIP regulates myocardial fatty acid oxidation via miR-33asignaling[J].American Journal of Physiology Heart&Circulatory Physiology,2016,311(1):64-75.
[18]Adevaandany M M,Gonzálezlucán M,Donapetrygarcía C,et al.Glycogen metabolism in humans[J].BBA Clinical,2016,5:85-100.
[19]Buervenich S,Carmine A,Galter D,et al.A rare truncating mutation in ADH1C(G78Stop)shows significant association with Parkinson disease in a large international sample.[J].Archives of Neurology,2005,62(1):74-78.
[20]韩学杰,李成卫.冠心病临床药对新用[M].中国医药科技出版社,2005:369.
[21]Nemmani K V S,Ramarao P.Ginseng total saponin potentiates acute U-50,488H-induced analgesia and inhibits tolerance to U-50,488H-induced analgesia in mice[J].Pharmacology Biochemistry&Behavior,2002,72(1-2):1-6.
[2]贾蔷,唐仕欢,石作荣,等.从中医气血理论探析“脑心同治”[J].世界中医药,2014(10):1293-1295.
[3]王冬菊.心脑血管疾病流行概况及主要影响因素[J].预防医学论坛,2016,22(01):71-75.
[4]郭优勤,魏桂林,钟秋明,等.浅谈人参的临床应用[J].中国现代药物应用,2011,05(7):128-129.
[5]刘会艳.黄芪及其制剂的药理作用和临床应用[J].内蒙古中医药,2012,31(22):44-45.
[6]许海玉,刘振明,付岩,等.中药整合药理学计算平台的开发与应用[J].中国中药杂志,2017,42(18):3633-3638.
[7]Lipinski C A,Lombardo F,Dominy B W,et al.Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings 1[J].Advanced Drug Delivery Reviews,2001,46(1-3):3-26.
[8]Gaignard Pauline,Liere Philippe,Thérond Patrice,et al.Role of Sex Hormones on Brain Mitochondrial Function,with Special Reference to Aging and Neurodegenerative Diseases[J].Frontiers in Aging Neuroscience,2017,9:406.
[9]宋齐.人参化学成分和药理作用研究进展[J].人参研究,2017,29(2):47-54.
[10]曹玉冰.黄芪甲苷的药理作用及其机制的研究进展[J].现代药物与临床,2017,32(05):954-960.
[11]Fernandes-Rosa F L,Boulkroun S,Zennaro M C.Somatic and inherited mutations in primary aldosteronism[J].Journal of Molecular Endocrinology,2017,59(1):47-63.
[12]Hu H J,Song M.Disrupted Ionic Homeostasis in Ischemic Stroke and New Therapeutic Targets.[J].J Stroke Cerebrovasc Dis,2017,26(12):2706-2719.
[13]Nd D G,Vega R B,Kelly D P.Mitochondrial biogenesis and dynamics in the developing and diseased heart.[J].Genes&Development,2015,29(19):1981-1991.
[14]Fan X,Xie J,Tian J.Reducing Cardiac Fibrosis:Na/K-ATPase Signaling Complex as a Novel Target[J].Cardiovasc Pharm Open Access,2017,6(1):1-12.
[15]Heslegrave A J,Hussain K.Novel insights into fatty acid oxidation,amino acid metabolism,and insulin secretion from studying patients with loss of function mutations in 3-hydroxyacyl-CoA dehydrogenase[J].Journal of Clinical Endocrinology&Metabolism,2013,98(2):496.
[16]Malik B,Fernandes C,Killick R,et al.Oligomeric amyloid-βpeptide affects the expression of genes involved in steroid and lipid metabolism in primary neurons.[J].Neurochemistry International,2012,61(3):321-333.
[17]Chen J,Young M E,Chatham J C,et al.TXNIP regulates myocardial fatty acid oxidation via miR-33asignaling[J].American Journal of Physiology Heart&Circulatory Physiology,2016,311(1):64-75.
[18]Adevaandany M M,Gonzálezlucán M,Donapetrygarcía C,et al.Glycogen metabolism in humans[J].BBA Clinical,2016,5:85-100.
[19]Buervenich S,Carmine A,Galter D,et al.A rare truncating mutation in ADH1C(G78Stop)shows significant association with Parkinson disease in a large international sample.[J].Archives of Neurology,2005,62(1):74-78.
[20]韩学杰,李成卫.冠心病临床药对新用[M].中国医药科技出版社,2005:369.
[21]Nemmani K V S,Ramarao P.Ginseng total saponin potentiates acute U-50,488H-induced analgesia and inhibits tolerance to U-50,488H-induced analgesia in mice[J].Pharmacology Biochemistry&Behavior,2002,72(1-2):1-6.