Wnt/PCP信号通路中RhoA mRNA与AECOPD中医证型的相关性
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摘要
目的:探讨平面细胞极性(Wnt/PCP)信号通路中Ras homolog gene family member A(RhoA) mRNA与慢性阻塞性肺疾病急性加重期(AECOPD)中医证型的相关性,尝试为AECOPD中医证型诊断提供客观标准。方法:收集AECOPD患者100例,分为痰浊壅肺证、痰热郁肺证、痰蒙神窍证、肺肾气虚证、阳虚水泛证5个组别,每组20例。正常组15例。所有入选患者空腹抽取静脉血,血液总RNA的提取采用试剂盒,按说明书进行操作,采用实时荧光定量聚合酶链式反应(Real-time PCR)检测RhoA基因在AECOPD中医证型患者血液中mRNA的表达量,并探讨其相关性。结果:痰热郁肺组和痰蒙神窍组比较无差异;与正常组比较,痰浊壅肺组、痰热郁肺组、痰蒙神窍组、肺肾气虚组和阳虚水泛组中RhoA mRNA表达量明显增高(P <0. 01); 5组证型间RhoA mRNA表达量,痰浊壅肺组<痰热郁肺组/痰蒙神窍组<肺肾气虚组<阳虚水泛组。RhoA mRNA相对表达量与AECOPD 5组中医证型之间存在直线线性相关关系,且呈正性相关。结论:Wnt/PCP信号通路中RhoA mRNA的相对表达量在5组中医证型中存在显著差异,可能给AECOPD中医证型诊断提供一定的客观标准,并揭示其疾病严重程度。
Objective: To investigate the correlation between Ras homolog gene family member A( RhoA) gene in Wnt/PCP signaling pathway and acute exacerbation chronic obstructive pulmonary disease( AECOPD) traditional Chinese medicine( TCM) syndrome,attempting to provide an objective standard for the diagnosis of AECOPD TCM syndrome. Method: The 100 AECOPD patients were collected and divided into 5 groups: phlegm turbid obstructing lung syndrome,,phlegm-heat obstructing lung syndrome,syndrome of orifices confused by phlegm,deficiency of pulmonary and renal Qi,and edema due to yang deficiency,with 20 people in each group. 15 normal people were selected as a normal control group. All patients received fasting hemospasia,using a kit to extract blood total RibonucleicA( RNA) according to instructions. Real-time quantitative polymerase chain reaction( Real-time PCR) was adopted to detect the mRNA expression of RhoA gene in blood of patients with AECOPD TCM syndrome,and to explore the correlation. Result: There was no difference between phlegm-heat obstructing lung syndrome group and syndrome of orifices confused by phlegm group. The mRNA expression of RhoA gene in phlegm turbid obstructing lung syndrome group,phlegm-heat obstructing lung syndrome group,syndrome of orifices confused by phlegm group,deficiency of pulmonary and renal Qi group,and edema due to Yang deficiency group were significantly higher than that in normal group( P < 0. 01). The mRNA expression of RhoA gene in five TCM syndrome groups was as follows: phlegm turbid obstructing lung syndrome < phlegm-heat obstructing lung syndrome/syndrome of orifices confused by phlegm < deficiency of pulmonary renal Qi < edema due to Yang deficiency. There was a positive linear correlation between the relative expression of RhoA mRNA and above five AECOPD TCM syndrome groups. Conclusion: The significant difference in mRNA relative expression of RhoA gene in Wnt/PCP signaling pathway among the five AECOPD TCM syndrome groups may provide some objective diagnostic criteria for AECOPD TCM syndromes and reveal their disease severity.
Objective: To investigate the correlation between Ras homolog gene family member A( RhoA) gene in Wnt/PCP signaling pathway and acute exacerbation chronic obstructive pulmonary disease( AECOPD) traditional Chinese medicine( TCM) syndrome,attempting to provide an objective standard for the diagnosis of AECOPD TCM syndrome. Method: The 100 AECOPD patients were collected and divided into 5 groups: phlegm turbid obstructing lung syndrome,,phlegm-heat obstructing lung syndrome,syndrome of orifices confused by phlegm,deficiency of pulmonary and renal Qi,and edema due to yang deficiency,with 20 people in each group. 15 normal people were selected as a normal control group. All patients received fasting hemospasia,using a kit to extract blood total RibonucleicA( RNA) according to instructions. Real-time quantitative polymerase chain reaction( Real-time PCR) was adopted to detect the mRNA expression of RhoA gene in blood of patients with AECOPD TCM syndrome,and to explore the correlation. Result: There was no difference between phlegm-heat obstructing lung syndrome group and syndrome of orifices confused by phlegm group. The mRNA expression of RhoA gene in phlegm turbid obstructing lung syndrome group,phlegm-heat obstructing lung syndrome group,syndrome of orifices confused by phlegm group,deficiency of pulmonary and renal Qi group,and edema due to Yang deficiency group were significantly higher than that in normal group( P < 0. 01). The mRNA expression of RhoA gene in five TCM syndrome groups was as follows: phlegm turbid obstructing lung syndrome < phlegm-heat obstructing lung syndrome/syndrome of orifices confused by phlegm < deficiency of pulmonary renal Qi < edema due to Yang deficiency. There was a positive linear correlation between the relative expression of RhoA mRNA and above five AECOPD TCM syndrome groups. Conclusion: The significant difference in mRNA relative expression of RhoA gene in Wnt/PCP signaling pathway among the five AECOPD TCM syndrome groups may provide some objective diagnostic criteria for AECOPD TCM syndromes and reveal their disease severity.
引文
[1]何权瀛,谭星宇.慢性阻塞性肺疾病的诊治进展[J].中国厂矿医学,2008,21(2):129-132.
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[9]余朝骏,侯键,陈楠,等.肺胀各证胸部平片X线征像及与血气分析关系的研究[J].中国中西医结合急救杂志,1999,6(11):493-498.
[10] Kikuchi A,Yamamoto H,Kishida S. Multiplicity of the interactions of Wnt proteins and their receptors[J].Cell Signal,2006,19(4):659-671.
[11] Van Scoyk M,Randall J,Sergew A,et al. Wnt signaling pathway and lung disease[J]. Transl Res,2008,151(4):175-180.
[12]侯成千,梁卫红,王军. Rho蛋白和细胞骨架的关系[J].生命的化学,2007,27(2):130-133.
[13] Pirone D M. An inhibitory role for FAK in regulating proliferation:a link between limited adhesion and Rho AROCK signaling[J]. J Cell Biol,2006,174(2):277-288.
[14] TAN J L. Cells lying on a bed of microneedles:an approach to isolate mechanical force[J]. Proc Natl Acad Sci USA,2003,100:1484-1489.
[15] Michele A,Christopher W S. Mechanotransduction in development:a growing role for contractility[J]. Nat Rev Mol Cell Biol,2009,10(1):34-43.
[16] Wodnicka M C,Burridge K. Rho-stimulated contractility drives the formation of stress fibers and focal adhesions[J]. J Cell Biol,1996(133):1403-1415.
[2]姜良铎.中医肺系病证研究进展[J].环球中医药,2009,2(3):164-172.
[3]周仲瑛.中医内科学[M].北京:中国中医药出版社,2007:112-119.
[4]于晓敏,王辰,王军. Wnt信号通路的生物学效应及其与肺部疾病的关系[J].首都医科大学学报,2009,30(2):182-184.
[5] Sasaki M,Kashima M,Ito T,et al. Effect of heparin and related glyco-saminoglycan on PDGF-induced lung fibroblast proliferation,chemotactic response and matrix metalloproteinases activity[J]. Mediat Inflamm,2000,9(2):85-91.
[6] Araujo B B,Dolhnikoff M,Silva L F,et al. Extracellular matrix components and regulators in the airway smooth muscle in asthma[J]. Eur Respir J,2008,32(1):61-69.
[7]中华医学会呼吸病学分会慢性阻塞性肺疾病学组.慢性阻塞性肺疾病诊治指南(2013年修订版)[J].中华结核和呼吸杂志,2013,36(4):255-264.
[8]焉石,刘振.慢性阻塞性肺疾病中医辨证分型与白细胞计数、肺功能、血气分析的相关性研究[J].中医药信息,2015,32(3):70-72.
[9]余朝骏,侯键,陈楠,等.肺胀各证胸部平片X线征像及与血气分析关系的研究[J].中国中西医结合急救杂志,1999,6(11):493-498.
[10] Kikuchi A,Yamamoto H,Kishida S. Multiplicity of the interactions of Wnt proteins and their receptors[J].Cell Signal,2006,19(4):659-671.
[11] Van Scoyk M,Randall J,Sergew A,et al. Wnt signaling pathway and lung disease[J]. Transl Res,2008,151(4):175-180.
[12]侯成千,梁卫红,王军. Rho蛋白和细胞骨架的关系[J].生命的化学,2007,27(2):130-133.
[13] Pirone D M. An inhibitory role for FAK in regulating proliferation:a link between limited adhesion and Rho AROCK signaling[J]. J Cell Biol,2006,174(2):277-288.
[14] TAN J L. Cells lying on a bed of microneedles:an approach to isolate mechanical force[J]. Proc Natl Acad Sci USA,2003,100:1484-1489.
[15] Michele A,Christopher W S. Mechanotransduction in development:a growing role for contractility[J]. Nat Rev Mol Cell Biol,2009,10(1):34-43.
[16] Wodnicka M C,Burridge K. Rho-stimulated contractility drives the formation of stress fibers and focal adhesions[J]. J Cell Biol,1996(133):1403-1415.