组织工程支架和骨髓间充质干细胞在脊髓损伤修复中的应用
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摘要
脊髓损伤后再生及修复困难,骨髓间充质干细胞来源丰富,可稳定培养扩增,具有多分化潜能,免疫原性弱,自体及异体移植对脊髓损伤修复均具有明显作用。但移植后存活率较低,作用有限。组织工程支架可填充脊髓空洞,改善脊髓微环境,模拟脊髓原有结构,携带药物和细胞因子,提高移植细胞的存活率及分化,且其设计理念不断更新。
It is difficult for the regeneration and repair of the spinal cord injury.The bone marrow mesenchymal stem cells,with abundant source and stable culture and easy amplification and multiple differentiation potential and weak immunogenicity were applied in autologous and allogeneic transplantation to repair spinal cord injury,but the survival rate of transplanted cells was low,the effect is limited.Tissue engineering scaffolds can be adapted to the spinal cavity,improve the microenvironment of the spinal cord,simulate the original structure of the spinal cord,carry drugs and cytokines,improve the survival rate and differentiation of transplanted cells,and the design idea is constantly updated.In this study,the combination of tissue engineering scaffold with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury was reviewed.
It is difficult for the regeneration and repair of the spinal cord injury.The bone marrow mesenchymal stem cells,with abundant source and stable culture and easy amplification and multiple differentiation potential and weak immunogenicity were applied in autologous and allogeneic transplantation to repair spinal cord injury,but the survival rate of transplanted cells was low,the effect is limited.Tissue engineering scaffolds can be adapted to the spinal cavity,improve the microenvironment of the spinal cord,simulate the original structure of the spinal cord,carry drugs and cytokines,improve the survival rate and differentiation of transplanted cells,and the design idea is constantly updated.In this study,the combination of tissue engineering scaffold with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury was reviewed.
引文
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[14]王东,张建军,杨卫山.NgR基因沉默骨髓间充质干细胞浒旺细胞支架复合体的构建[J].中国组织工程研究,2012,16(25):4591-4595.
[15]Krsko P,McCann TE,Thach TT,et al.Length-scale mediated adhesion and directed growth of neural cells by surface-patterned polyethylene glycol)hydrogels[J].Biomaterials,2009,30(5):721-729.
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[17]卢丽雅,张巍,曾园山,等.神经营养素-3基因修饰骨髓间充质干细胞的明胶海绵圆柱体支架移植促进大鼠全横断脊髓损伤部分结构和功能修复的研究[J].中国康复医学杂志,2011,26(7):603-608.
[18]Huang JH,Hu XY,Lu L,et al.Electrical regulation of Schwann cells using conductive polypyrrole/chitosan polymers[J].J Biomed Mater Res A,2010,93(1):164-174.
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[21]Shen YX,Qian YQ,Zhang HX,et al.Guidance of Olfactory Ensheathing Cell Growth and Migration on Electrospun Silk Fibroin Scaffolds[J].Cell Transplantation,2010,19(2):147-157.
[22]Ravichandran R,Griffith M,Phopase J.Applications of selfassembling peptide scaffolds in regenerative medicine:the way to the clinic[J].J Mater Chem B,2014,2(48):8466-8478.
[23]Dionigi C,Posati T,Benfenati V,et al.A nano structured conductive bio-composite of silk fibroin-single walled carbon nanotubes[J].J Mater Chem,2014,2(10):1424-1431.
[24]李宁,刘斌,戎利民,等.静电纺丝聚乳酸聚乙醇酸/聚乙二醇纳米纤维作为组织工程支架的体外细胞相容性研究[J].中华创伤骨科杂志,2012,14(3):241-245.
[25]Fansa H,Schneider W,Wolf G,et al.Host responses after acellular muscle basal lamina allografting used as a matrix for tissue engineered nerve grafts[J].Transplantation,2002,74(3):381-387.
[26]魏祥科,文益民,张涛,等.BMSCs联合去细胞肌肉生物支架修复大鼠脊髓半切损伤的实验研究[J].中国修复重建外科杂志,2012,26(11):1362-1368.
[27]Onuma-Ukegawa M,Bhatt K,Hirai T,et al.Bone marrow stromal cells combined with a honeycomb collagen sponge facilitate neurite elongation in vitro and neural restoration in the hemisected rat spinal cord[J].Cell Transplant,2015,24(7):1283-1297.
[28]Wang X,Li Y,Gao Y,et al.Combined use of spinal cord-mimicking partition type scaffold architecture and neurotrophin-3 for surgical repair of completely transected spinal cord in rats[J].J Biomater Sci Polym Ed.2013,24(8):927-939.
[29]Sasaki H,Tanaka N,Nakanishi K,et al.Therapeutic effects with magnetic targeting of bone marrow stromal cells in a rat spinal cord injury model[J].Spine(Phila Pa 1976),2011,36(12):933-938.
[30]Amr SM,Gouda A,Koptan WT,et al.Bridging defects in chronic spinal cord injury using peripheral nerve grafts combined with a chitosan-laminin scaffold and enhancing regeneration through them by co-transplantation with bone-marrow-derived mesenchymal stem cells:case series of 14 patients[J].J Spinal Cord Med,2014,37(1):54-71.
[2]Dasari VR,Veeravalli KK,Dinh DH.Mesenchymal stem cells in the treatment of spinal cord injuries:a review[J].World J Stem Cells,2014,6(2):120-133.
[3]Choi JS,Leem JW,Lee KH,et al.Effects of human mesenchymal stem cell transplantation combined with polymer on functional recovery following spinal cord hemisection in rats[J].Korean J Physiol Pharmacol,2012,16(6):405-411.
[4]Shin DA,Kim JM,Kim HI,et al.Comparison of functional and histological outcomes after intralesional,intracisternal,and intravenous transplantation of human bone marrow-derived mesenchymal stromal cells in a rat model of spinal cord injury[J].Acta Neurochir(Wien),2013,155(10):1943-1950.
[5]Barriga A,Medrano M,De-Juan J,et al.Intravenous infusion of adult adiposetissue stem cells for repairing spinal cord ischaemic lesions.An experimental study on animals[J].Rev Esp Cir Ortop Traumatol,2013,57(2):89-94.
[6]Ukegawa M,Bhatt K,Hirai T,et al.Bone marrow stromal cells combined with a honeycomb collagen sponge facilitate neurite elongation in vitro and neural restoration in the hemisected rat spinal cord[J].Cell Transplant,2015,24(7):1283-1297.
[7]Chen X,Yang Y,Yao J,et al.Bone marrow stromal cells-loaded chitosan conduits promote repair of complete transection injury in rat spinal cord[J].J Mater Sci:Mater Med,2011,22(10):2347-2356.
[8]Ribeiro-Samy S,Silva NA,Correlo VM,et al.Development and Characterization of a PHB-HV-based 3D Scaffold for a Tissue Engineering and Cell-therapy Combinatorial Approach for Spinal Cord Injury Regeneration[J].Macromol Biosci,2013,13(11):1576-1592.
[9]Ansorena E,De Berdt P,Ucakar B,et al.Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury[J].Int J Pharm,2013,455(1-2):148-158.
[10]McKay CA,Pomrenke RD,McLane JS,et al.An Injectable,Calcium Responsive Composite Hydrogel for the Treatment of Acute Spinal Cord Injury[J].ACS Appl Mater Interfaces,2014,6(3):1424-1438.
[11]Gunther MI,Weidner N,Müller R,et al.Cell-seeded alginate hydrogel scaffolds promote directed linear axonal regeneration in the injured rat spinal cord[J].Acta Biomater,2015,27:140-150.
[12]King VR,Alovskaya A,Wei DY,et al.The use of injectable forms of fibrin and fibronectin to support axonal ingrowth after spinal cord injury[J].Biomaterials,2010,31(15):4447-4456.
[13]Pertici V,Trimaille T,Laurin J,et al.Repair of the injured spinal cord by implantation of a synthetic degradable block copolymer in rat[J].Biomaterials,2014,35(24):6248-6258.
[14]王东,张建军,杨卫山.NgR基因沉默骨髓间充质干细胞浒旺细胞支架复合体的构建[J].中国组织工程研究,2012,16(25):4591-4595.
[15]Krsko P,McCann TE,Thach TT,et al.Length-scale mediated adhesion and directed growth of neural cells by surface-patterned polyethylene glycol)hydrogels[J].Biomaterials,2009,30(5):721-729.
[16]Estrada V,Brazda N,Schmitz C,et al.Long-lasting significant functional improvement in chronic severe spinal cord injury following scar resection and polyethylene glycol implantation[J].Neurobiol Dis,2014,67:165-179.
[17]卢丽雅,张巍,曾园山,等.神经营养素-3基因修饰骨髓间充质干细胞的明胶海绵圆柱体支架移植促进大鼠全横断脊髓损伤部分结构和功能修复的研究[J].中国康复医学杂志,2011,26(7):603-608.
[18]Huang JH,Hu XY,Lu L,et al.Electrical regulation of Schwann cells using conductive polypyrrole/chitosan polymers[J].J Biomed Mater Res A,2010,93(1):164-174.
[19]Crapo PM,Medberry CJ,Reing JE,et al.Biologic scaffolds composed of central nervous system extracellular matrix[J].Biomaterials,2012,33(13):3539-3547.
[20]Liu J,Chen J,Liu B,et al.Acellular spinal cord scaffold seeded with mesenchymal stem cells promotes long-distance axon regeneration and functional recovery in spinal cord injured rats[J].J Neurol Sci,2013,325(1-2):127-136.
[21]Shen YX,Qian YQ,Zhang HX,et al.Guidance of Olfactory Ensheathing Cell Growth and Migration on Electrospun Silk Fibroin Scaffolds[J].Cell Transplantation,2010,19(2):147-157.
[22]Ravichandran R,Griffith M,Phopase J.Applications of selfassembling peptide scaffolds in regenerative medicine:the way to the clinic[J].J Mater Chem B,2014,2(48):8466-8478.
[23]Dionigi C,Posati T,Benfenati V,et al.A nano structured conductive bio-composite of silk fibroin-single walled carbon nanotubes[J].J Mater Chem,2014,2(10):1424-1431.
[24]李宁,刘斌,戎利民,等.静电纺丝聚乳酸聚乙醇酸/聚乙二醇纳米纤维作为组织工程支架的体外细胞相容性研究[J].中华创伤骨科杂志,2012,14(3):241-245.
[25]Fansa H,Schneider W,Wolf G,et al.Host responses after acellular muscle basal lamina allografting used as a matrix for tissue engineered nerve grafts[J].Transplantation,2002,74(3):381-387.
[26]魏祥科,文益民,张涛,等.BMSCs联合去细胞肌肉生物支架修复大鼠脊髓半切损伤的实验研究[J].中国修复重建外科杂志,2012,26(11):1362-1368.
[27]Onuma-Ukegawa M,Bhatt K,Hirai T,et al.Bone marrow stromal cells combined with a honeycomb collagen sponge facilitate neurite elongation in vitro and neural restoration in the hemisected rat spinal cord[J].Cell Transplant,2015,24(7):1283-1297.
[28]Wang X,Li Y,Gao Y,et al.Combined use of spinal cord-mimicking partition type scaffold architecture and neurotrophin-3 for surgical repair of completely transected spinal cord in rats[J].J Biomater Sci Polym Ed.2013,24(8):927-939.
[29]Sasaki H,Tanaka N,Nakanishi K,et al.Therapeutic effects with magnetic targeting of bone marrow stromal cells in a rat spinal cord injury model[J].Spine(Phila Pa 1976),2011,36(12):933-938.
[30]Amr SM,Gouda A,Koptan WT,et al.Bridging defects in chronic spinal cord injury using peripheral nerve grafts combined with a chitosan-laminin scaffold and enhancing regeneration through them by co-transplantation with bone-marrow-derived mesenchymal stem cells:case series of 14 patients[J].J Spinal Cord Med,2014,37(1):54-71.