喷施烯效唑调控大豆根部解剖结构缓解盐逆境伤害
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摘要
以大豆栽培品种"中黄13"为试材,检验大豆幼苗叶面喷施烯效唑(S_(3307))通过调控根部解剖结构变化缓解盐害作用。结果表明:叶面喷施S_(3307)后,盐逆境下降低的株高、根长、根及地上部的干重和鲜重、相对生长速率、叶面积和髓部直径等参数均回升;而增加的Cl~-含量、根/冠比、皮层厚度和射线长度等参数均表现为下调至对照与盐逆境植株之间;变形、凹陷的表皮细胞恢复至对照水平,导管中没有发现侵填体;喷施S_(3307)通过降低皮层厚度的方式减少根系总新陈代谢的消耗,缩短水分通过皮层向维管柱运输的距离;髓部直径的增加有利于营养物质的储存;射线长度降低能够缩短根系养分的横向运输距离,能够提高水分和营养物质的吸收和运输效率;根部和地上部分Cl~-含量显著下调极大减少了盐逆境对大豆幼苗的氯毒害;喷施S_(3307)能够通过调控根部解剖结构缓解盐逆境对大豆的伤害作用。
We tested whether exogenous uniconazole(S_(3307)) would ameliorate salt injury to soybean seedlings by regulating anatomical structure in roots, withGlycine maxcultivar ‘ Zhong-huang 13' as the experimental material. The results showed that after spraying S_(3307) on salt-stressed soybean, the salt-induced declined parameters, including plant height, root length, dryand fresh weight of roots and shoots, relative growth rate, leaf area, and pith diameter, weremarkedly recovered, while the salt-induced ascended parameters, including Cl~-content, root/shoot ratio, cortex thickness and ray length, were decreased to levels between that in the controland the salt-stressed seedlings. Partial deformation and invagination of salt-stressed epidermalcells recovered to the control level, with no tylosis being found in vessel elements. Spraying S_(3307) reduced the metabolic cost of roots by decreasing cortex thickness in roots, and shortened the dis-tance of water absorption from root to vascular cylinder. Additionally, increased pith diameter wasbeneficial to nutrient storage, and reduced ray length shortened the lateral transport distance ofnutrients in roots. Meanwhile, a significant reduction in Cl~-contents in roots and shoots couldgreatly relieve the chlorine toxicity to soybean seedlings under salt stress. In conclusion, S_(3307) application could ameliorate salt injury by regulating anatomical structure in soybean roots.
We tested whether exogenous uniconazole(S_(3307)) would ameliorate salt injury to soybean seedlings by regulating anatomical structure in roots, withGlycine maxcultivar ‘ Zhong-huang 13' as the experimental material. The results showed that after spraying S_(3307) on salt-stressed soybean, the salt-induced declined parameters, including plant height, root length, dryand fresh weight of roots and shoots, relative growth rate, leaf area, and pith diameter, weremarkedly recovered, while the salt-induced ascended parameters, including Cl~-content, root/shoot ratio, cortex thickness and ray length, were decreased to levels between that in the controland the salt-stressed seedlings. Partial deformation and invagination of salt-stressed epidermalcells recovered to the control level, with no tylosis being found in vessel elements. Spraying S_(3307) reduced the metabolic cost of roots by decreasing cortex thickness in roots, and shortened the dis-tance of water absorption from root to vascular cylinder. Additionally, increased pith diameter wasbeneficial to nutrient storage, and reduced ray length shortened the lateral transport distance ofnutrients in roots. Meanwhile, a significant reduction in Cl~-contents in roots and shoots couldgreatly relieve the chlorine toxicity to soybean seedlings under salt stress. In conclusion, S_(3307) application could ameliorate salt injury by regulating anatomical structure in soybean roots.
引文
姜妍,冷建田,费志宏,等.2009.广适应大豆品种中黄13的光周期反应.大豆科学,28(3):377-381.
李宁毅,吉振,时彦平.2011.烯效唑(S3307)对矮牵牛幼苗耐盐性的调节效应.沈阳农业大学学报,42(6):668-671.
李宁毅,时彦平,王吉振.2012.水分胁迫下烯效唑对百日草幼苗光合特性及叶解剖结构的影响.西北植物学报,32(8):1626-1631.
李煜昶,岳铭秀,董景华.1998.烯效唑对大豆的增产作用.天津农业科学,4(2):15-17.
刘丽琴,张永清,李鑫,等.2017.烯效唑浸种对干旱胁迫下红小豆生长及其根系生理特性的影响.西北植物学报,37(1):144-153.
孟娜,徐航,魏明,等.2017.叶面喷施烯效唑对盐胁迫下大豆幼苗的缓解效应.西北植物学报,37(10):1988-1995.
屈娅娜,於丙军.2008.氯离子通道抑制剂对盐胁迫下野生和栽培大豆幼苗离子含量等生理指标的影响.南京农业大学学报,31(2):17-24.
王洪新,胡志昂,钟敏,等.1997.盐渍条件下野大豆群体的遗传分化和生理适应:同工酶和随机扩增多态DNA研究.植物学报,39(l):34-42.
伍应保.2008.栽培大豆和滩涂野大豆杂交后代JB185的耐盐生理与分子机理(硕士学位论文).南京:南京农业大学.
周强,李萍,曹金花,等.2007.测定植物体内氯离子含量的滴定法和分光光度法比较.植物生理学通迅,43(6):1163-1166.
朱俊义,杨忠顺,梁泽本,等.2003.大豆属植物根的初生结构及系统演化.东北师大学报:自然科学版,35(4):112-116.
Akcin TA,Akcin A,Yalcin E.2017.Anatomical changes induced by salinity stress inSalicornia freitagii(Amaranthaceae).Brazilian Journal of Botany,40:1013-1018.
Barbier-Brygoo H,Vinauger M,Colcombet J,et al.2000.Anion channels in higher plants:Functional characterization,molecular structure and physiological role.Biochimica et Biophysica Acta:Biomembranes,1465:199-218.
Chi TN,Agorio A,Jossier M,et al.2016.Characterization of the chloride channel-like,AtCLCg,involved in chloride tolerance inArabidopsis thaliana.Plant and Cell Physiology,57:764-775.
Chimungu JG,Brown KM,Lynch JP.2014a.Large root cortical cell size improves drought tolerance in maize.Plant Physiology,166:2166-2178.
Chimungu JG,Brown KM,Lynch JP.2014b.Reduced root cortical cell file number improves drought tolerance in maize.Plant Physiology,166:1943-1955.
Guo JS,Zhou Q,Li XJ,et al.2017.Enhancing NO-supply confers NaCl tolerance by adjusting Cl-uptake and transport inG.max&G.soja.Journal of Soil Science and Plant Nutrition,17:194-204.
Huang MJ,Fang Y,Liu Y,et al.2015.Using proteomic analysis to investigate uniconazole-induced phytohormone variation and starch accumulation in duckweed(Landoltia punctata).BMC Biotechnology,15:81.
Jiang B,Nan H,Gao Y,et al.2014.Allelic combinations of soybean maturity lociE1,E2,E3 andE4 result in diver sity of maturity and adaptation to different latitudes.PLoSOne,9:e106042.
Liu Y,Fang Y,Huang MJ,et al.2015.Uniconazole-induced starch accumulation in the bioenergy crop duckweed(Landoltia punctata).II:Transcriptome alterations of pathways involved in carbohydrate metabolism and endogenous hormone crosstalk.Biotechnology for Biofuels,8:64.
Luo QY,Yu BJ,Liu YL.2005.Differential sensitivity to chloride and sodium ions in seedlings ofGlycine maxandGlycine sojaunder NaCl stress.Journal of Plant Physiology,162:1003-1012.
Meister R,Rajani MS,Ruzicka D,et al.2014.Challenges of modifying root traits in crops for agriculture.Trends in Plant Science,19:779-788.
Meng N,Yu BJ,Guo JS.2016.Ameliorative effects of inoculation withBradyrhizobium japonicumonGlycine maxand Glycine sojaseedlings under salt stress.Plant Growth Regulation,81:137-147.
Meng N,Yu BJ.2018.Proteomics-based investigation of saltresponsive mechanisms in roots ofBradyrhizobium japonicum-inoculatedGlycine maxandGlycine sojaseedlings.Journal of Plant Growth Regulation,37:266-277.
Munns R,Tester M.2008.Mechanisms of salinity tolerance.Annual Review of Plant Biology,59:651-681.
Schmutz J,Cannon SB,Schlueter J,et al.2010.Genome sequence of the palaeopolyploid soybean.Nature,463:178-183.
Teakle NL,Tyerman SD.2010.Mechanisms of Cl-transport contributing to salt tolerance.Plant,Cell and Environment,33:566-589.
Vincent D,Ergul A,Bohlman MC,et al.2007.Proteomic analysis reveals differences betweenVitis viniferaL.‘Chardonnay’and‘Cabernet Sauvignon’and their responses to water deficit and salinity.Journal of Experimental Botany,58:1873-1892.
Zhang MC,Duan LS,Tian XL,et al.2007.Uniconazole-induced tolerance of soybean to water deficit stress in relation to changes in photosynthesis,hormones and antioxidant system.Journal of Plant Physiology,164:709-717.
李宁毅,吉振,时彦平.2011.烯效唑(S3307)对矮牵牛幼苗耐盐性的调节效应.沈阳农业大学学报,42(6):668-671.
李宁毅,时彦平,王吉振.2012.水分胁迫下烯效唑对百日草幼苗光合特性及叶解剖结构的影响.西北植物学报,32(8):1626-1631.
李煜昶,岳铭秀,董景华.1998.烯效唑对大豆的增产作用.天津农业科学,4(2):15-17.
刘丽琴,张永清,李鑫,等.2017.烯效唑浸种对干旱胁迫下红小豆生长及其根系生理特性的影响.西北植物学报,37(1):144-153.
孟娜,徐航,魏明,等.2017.叶面喷施烯效唑对盐胁迫下大豆幼苗的缓解效应.西北植物学报,37(10):1988-1995.
屈娅娜,於丙军.2008.氯离子通道抑制剂对盐胁迫下野生和栽培大豆幼苗离子含量等生理指标的影响.南京农业大学学报,31(2):17-24.
王洪新,胡志昂,钟敏,等.1997.盐渍条件下野大豆群体的遗传分化和生理适应:同工酶和随机扩增多态DNA研究.植物学报,39(l):34-42.
伍应保.2008.栽培大豆和滩涂野大豆杂交后代JB185的耐盐生理与分子机理(硕士学位论文).南京:南京农业大学.
周强,李萍,曹金花,等.2007.测定植物体内氯离子含量的滴定法和分光光度法比较.植物生理学通迅,43(6):1163-1166.
朱俊义,杨忠顺,梁泽本,等.2003.大豆属植物根的初生结构及系统演化.东北师大学报:自然科学版,35(4):112-116.
Akcin TA,Akcin A,Yalcin E.2017.Anatomical changes induced by salinity stress inSalicornia freitagii(Amaranthaceae).Brazilian Journal of Botany,40:1013-1018.
Barbier-Brygoo H,Vinauger M,Colcombet J,et al.2000.Anion channels in higher plants:Functional characterization,molecular structure and physiological role.Biochimica et Biophysica Acta:Biomembranes,1465:199-218.
Chi TN,Agorio A,Jossier M,et al.2016.Characterization of the chloride channel-like,AtCLCg,involved in chloride tolerance inArabidopsis thaliana.Plant and Cell Physiology,57:764-775.
Chimungu JG,Brown KM,Lynch JP.2014a.Large root cortical cell size improves drought tolerance in maize.Plant Physiology,166:2166-2178.
Chimungu JG,Brown KM,Lynch JP.2014b.Reduced root cortical cell file number improves drought tolerance in maize.Plant Physiology,166:1943-1955.
Guo JS,Zhou Q,Li XJ,et al.2017.Enhancing NO-supply confers NaCl tolerance by adjusting Cl-uptake and transport inG.max&G.soja.Journal of Soil Science and Plant Nutrition,17:194-204.
Huang MJ,Fang Y,Liu Y,et al.2015.Using proteomic analysis to investigate uniconazole-induced phytohormone variation and starch accumulation in duckweed(Landoltia punctata).BMC Biotechnology,15:81.
Jiang B,Nan H,Gao Y,et al.2014.Allelic combinations of soybean maturity lociE1,E2,E3 andE4 result in diver sity of maturity and adaptation to different latitudes.PLoSOne,9:e106042.
Liu Y,Fang Y,Huang MJ,et al.2015.Uniconazole-induced starch accumulation in the bioenergy crop duckweed(Landoltia punctata).II:Transcriptome alterations of pathways involved in carbohydrate metabolism and endogenous hormone crosstalk.Biotechnology for Biofuels,8:64.
Luo QY,Yu BJ,Liu YL.2005.Differential sensitivity to chloride and sodium ions in seedlings ofGlycine maxandGlycine sojaunder NaCl stress.Journal of Plant Physiology,162:1003-1012.
Meister R,Rajani MS,Ruzicka D,et al.2014.Challenges of modifying root traits in crops for agriculture.Trends in Plant Science,19:779-788.
Meng N,Yu BJ,Guo JS.2016.Ameliorative effects of inoculation withBradyrhizobium japonicumonGlycine maxand Glycine sojaseedlings under salt stress.Plant Growth Regulation,81:137-147.
Meng N,Yu BJ.2018.Proteomics-based investigation of saltresponsive mechanisms in roots ofBradyrhizobium japonicum-inoculatedGlycine maxandGlycine sojaseedlings.Journal of Plant Growth Regulation,37:266-277.
Munns R,Tester M.2008.Mechanisms of salinity tolerance.Annual Review of Plant Biology,59:651-681.
Schmutz J,Cannon SB,Schlueter J,et al.2010.Genome sequence of the palaeopolyploid soybean.Nature,463:178-183.
Teakle NL,Tyerman SD.2010.Mechanisms of Cl-transport contributing to salt tolerance.Plant,Cell and Environment,33:566-589.
Vincent D,Ergul A,Bohlman MC,et al.2007.Proteomic analysis reveals differences betweenVitis viniferaL.‘Chardonnay’and‘Cabernet Sauvignon’and their responses to water deficit and salinity.Journal of Experimental Botany,58:1873-1892.
Zhang MC,Duan LS,Tian XL,et al.2007.Uniconazole-induced tolerance of soybean to water deficit stress in relation to changes in photosynthesis,hormones and antioxidant system.Journal of Plant Physiology,164:709-717.