黄芪提取物对慢性非细菌性前列腺炎大鼠的作用及机制探讨
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摘要
目的:研究黄芪提取物对慢性非细菌性前列腺炎大鼠的治疗作用及机制。方法:60只SD大鼠随机分为6组,正常组、模型组、阳性药舍尼通组(100 mg·kg-1)和黄芪提取物低、中和高剂量组(100,200,400 mg·kg-1),每组10只,除正常组外,其余各组用角叉菜胶制备实验性的慢性非细菌性前列腺炎大鼠模型,每天ig给药1次,正常组和模型组给予蒸馏水,连续给药3周。观察黄芪提取物对大鼠前列腺指数(PI),前列腺特异性抗原(PSA)和前列腺组织中炎症因子肿瘤坏死因子-α(TNF-α),白介素-1β(IL-1β),环氧酶-2(COX-2),前列腺素E2(PEG2),转化生长因子-β1(TGF-β1)和结缔组织生长因子(CTGF)含量的影响,以及前列腺组织病理形态的变化。结果:与正常组比较,模型组明显升高大鼠PI和PSA,明显升高大鼠前列腺组织中TNF-α,IL-1β,COX-2,PEG2,TGF-β1和CTGF的水平,均具有显著的差异(P<0.01),炎症细胞浸润和间质纤维化明显;与模型组比较,黄芪提取物高、中、低剂量组均能明显降低慢性非细菌性前列腺模型大鼠PI和PSA(P<0.01),明显降低模型大鼠前列腺组织中TNF-α,IL-1β,COX-2,PEG2,TGF-β1和CTGF的水平(P<0.01),同时显著减轻了炎症细胞浸润和间质纤维化的程度。结论:本研究证实黄芪提取物对慢性非细菌性前列腺炎大鼠有明显的治疗作用。
Objective: To observe the effect and mechanism of Astragalic Radix extract on chronic nonbacterial prostatitis in rats. Method: Sixty SD rats were randomly divided into 6 groups: the normal group( distilled water),the model group( distilled water),the cernilton group( 100 mg·kg- 1),the low-,medium-and high-dose Astragalic Radix extract groups( 100,200 and 400 mg·kg- 1) of 10 rats each. The chronic nonbacterial prostatitis model was prepared in rats except those in the normal group by injecting carrageenan. The corresponding medicines were intragastrically administered to rats once daily for 3 weeks. The effects on levels of prostatic index( PI), prostate-specific antigen( PSA), tumor necrosis factor-α( TNF-α), interleukin-1β( IL-1β),cyclooxygenase-2( COX-2),prostaglandin E2( PEG2),transforming growth factor-β1( TGF-β1) and connective tissue growth factor( CTGF) were determined. The pathological changes of prostate tissue in rats were observed.Result: Compared with the normal group,the levels of PI,PSA,TNF-α,IL-1β,COX-2,PEG2,TGF-β1and CTGF increased in the model group( P < 0. 01). Moreover,the pathological results showed obvious inflammatory cell infiltration and fibrosis in the model group. Compared with model group,the levels of PI,PSA,TNF-α,IL-1β,COX-2,PEG2,TGF-β1and CTGF decreased in the Astragalic Radix extract groups( P < 0. 01). Meanwhile,the pathological morphology had good improvement. Conclusion: This study confirmed Astragalic Radix extract had therapeutically effective effect on chronic nonbacterial prostatitis in rats.
Objective: To observe the effect and mechanism of Astragalic Radix extract on chronic nonbacterial prostatitis in rats. Method: Sixty SD rats were randomly divided into 6 groups: the normal group( distilled water),the model group( distilled water),the cernilton group( 100 mg·kg- 1),the low-,medium-and high-dose Astragalic Radix extract groups( 100,200 and 400 mg·kg- 1) of 10 rats each. The chronic nonbacterial prostatitis model was prepared in rats except those in the normal group by injecting carrageenan. The corresponding medicines were intragastrically administered to rats once daily for 3 weeks. The effects on levels of prostatic index( PI), prostate-specific antigen( PSA), tumor necrosis factor-α( TNF-α), interleukin-1β( IL-1β),cyclooxygenase-2( COX-2),prostaglandin E2( PEG2),transforming growth factor-β1( TGF-β1) and connective tissue growth factor( CTGF) were determined. The pathological changes of prostate tissue in rats were observed.Result: Compared with the normal group,the levels of PI,PSA,TNF-α,IL-1β,COX-2,PEG2,TGF-β1and CTGF increased in the model group( P < 0. 01). Moreover,the pathological results showed obvious inflammatory cell infiltration and fibrosis in the model group. Compared with model group,the levels of PI,PSA,TNF-α,IL-1β,COX-2,PEG2,TGF-β1and CTGF decreased in the Astragalic Radix extract groups( P < 0. 01). Meanwhile,the pathological morphology had good improvement. Conclusion: This study confirmed Astragalic Radix extract had therapeutically effective effect on chronic nonbacterial prostatitis in rats.
引文
[1]Motrich R D,Maccioni M,Molina R,et al.Presence of INFgamma-secreting lymphocytes specific to prostate antigens in a group of chronic prostatitis patients[J].Clin Immunol,2005,116(2):149-157.
[2]陈国辉,黄文凤.黄芪的化学成分及药理作用研究进展[J].中国新药杂志,2008,17(17):1482-1485.
[3]Chen R Z,Cui L,Guo Y J,et al.In vivo study of four preparative extracts of Clematis terniflora D C.for antinociceptive activity and anti-inflammatory activity in rat model of carrageenan-induced chronic non-bacterial prostatitis[J].J Ethnopharmacol,2011,134(3):1018-1023.
[4]孙继红,胡梅,巫凤娟.花川保列颗粒对大鼠非菌性前列腺炎模型的实验研究[J].中国实验方剂学杂志,2010,16(4):135-138.
[5]叶勇,郭亚,徐杏昌.复方中药组方抗前列腺炎的实验研究[J].时珍国医国药,2012,23(9):2195-2196.
[6]王先进,钟山,张存明.大鼠慢性非细菌性前列腺炎模型的建立及病理组织学特征[J].中华泌尿外科杂志,2012,33(4):282-287.
[7]任钧国,王建业,李军梅.前列舒通胶囊对雌二醇诱导的慢性非细菌性前列腺炎大鼠T淋巴细胞亚群的影响[J].现代泌尿外科杂志,2013,18(3):233-236.
[8]Lu B Y,Cai H F,Huang W S,et al.Protective effect of bamboo shoot oil on experimental nonbacterial prostatitis in rats[J].Food Chem,2011,124(3):1017-1023.
[9]Tsunemori H,Sugimoto M,Xia Z,et al.Effect of the phytotherapeutic agent Eviprostat on inflammatory changes and cytokine production in a rat model of nonbacterial prostatitis[J].Urology,2011,77(7):e15-e20.
[10]Au R Y,Al-Talib,T K Au,et al.Avocado soybean unsaponifiables(ASU)suppress TNF-alpha,IL-1beta,COX-2,i NOS gene expression,and prostaglandin E2and nitric oxide production in articular chondrocytes and monocyte/macrophages[J].Osteoarthr Cartil,2007,15(11):1249-1255.
[11]Tahir M,Rehman M U,Lateef A,et al.Diosmin protects against ethanol-induced hepatic injury via alleviation of in ammation and regulation of TNF-alpha and NFkappa B activation[J].Alcohol,2013,47(2):131-139.
[12]Lu B Y,Cai H F,Huang W S,et al.Protective effect of bamboo shoot oil on experimental nonbacterial prostatitis in rats[J].Food Chem,2011,124(3):1017-1023.
[13]Liu R M,Gaston Pravia,KAG.Oxidative stress and glutathione in TGF-beta-Mediated brogenesis[J].Free Radic Biol Med,2010,48(1):1-15.
[14]Leask A,Abraham D J.TGF-beta signaling and the fibrotic response[J].FASEB J,2004,18(7):816-827.
[15]Borthwick L A,Wynn T A,Fisher A J.Cytokine mediated tissue fibrosis[J].Biochim Biophys Acta,2013,1832(7):1049-1060.
[16]魏伟,李晓辉,张洪泉,等.抗炎免疫药理学[M].北京:人民卫生出版社,2005:150.
[2]陈国辉,黄文凤.黄芪的化学成分及药理作用研究进展[J].中国新药杂志,2008,17(17):1482-1485.
[3]Chen R Z,Cui L,Guo Y J,et al.In vivo study of four preparative extracts of Clematis terniflora D C.for antinociceptive activity and anti-inflammatory activity in rat model of carrageenan-induced chronic non-bacterial prostatitis[J].J Ethnopharmacol,2011,134(3):1018-1023.
[4]孙继红,胡梅,巫凤娟.花川保列颗粒对大鼠非菌性前列腺炎模型的实验研究[J].中国实验方剂学杂志,2010,16(4):135-138.
[5]叶勇,郭亚,徐杏昌.复方中药组方抗前列腺炎的实验研究[J].时珍国医国药,2012,23(9):2195-2196.
[6]王先进,钟山,张存明.大鼠慢性非细菌性前列腺炎模型的建立及病理组织学特征[J].中华泌尿外科杂志,2012,33(4):282-287.
[7]任钧国,王建业,李军梅.前列舒通胶囊对雌二醇诱导的慢性非细菌性前列腺炎大鼠T淋巴细胞亚群的影响[J].现代泌尿外科杂志,2013,18(3):233-236.
[8]Lu B Y,Cai H F,Huang W S,et al.Protective effect of bamboo shoot oil on experimental nonbacterial prostatitis in rats[J].Food Chem,2011,124(3):1017-1023.
[9]Tsunemori H,Sugimoto M,Xia Z,et al.Effect of the phytotherapeutic agent Eviprostat on inflammatory changes and cytokine production in a rat model of nonbacterial prostatitis[J].Urology,2011,77(7):e15-e20.
[10]Au R Y,Al-Talib,T K Au,et al.Avocado soybean unsaponifiables(ASU)suppress TNF-alpha,IL-1beta,COX-2,i NOS gene expression,and prostaglandin E2and nitric oxide production in articular chondrocytes and monocyte/macrophages[J].Osteoarthr Cartil,2007,15(11):1249-1255.
[11]Tahir M,Rehman M U,Lateef A,et al.Diosmin protects against ethanol-induced hepatic injury via alleviation of in ammation and regulation of TNF-alpha and NFkappa B activation[J].Alcohol,2013,47(2):131-139.
[12]Lu B Y,Cai H F,Huang W S,et al.Protective effect of bamboo shoot oil on experimental nonbacterial prostatitis in rats[J].Food Chem,2011,124(3):1017-1023.
[13]Liu R M,Gaston Pravia,KAG.Oxidative stress and glutathione in TGF-beta-Mediated brogenesis[J].Free Radic Biol Med,2010,48(1):1-15.
[14]Leask A,Abraham D J.TGF-beta signaling and the fibrotic response[J].FASEB J,2004,18(7):816-827.
[15]Borthwick L A,Wynn T A,Fisher A J.Cytokine mediated tissue fibrosis[J].Biochim Biophys Acta,2013,1832(7):1049-1060.
[16]魏伟,李晓辉,张洪泉,等.抗炎免疫药理学[M].北京:人民卫生出版社,2005:150.