羊肉鉴伪技术的研究进展及其分析比较
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摘要
近年来,利用鸭肉、猪肉、貂肉、老鼠肉制造假羊肉新闻屡见不鲜,假羊肉的泛滥不仅干扰了市场秩序,还严重损害了养殖户和消费者的利益,因此有效利用羊肉鉴伪技术打击不法商家从事假羊肉生产经营活动,以维护市场秩序,保护消费者利益具有重要意义。随着仪器分析技术的发展,假羊肉的鉴别方法从传统的感官鉴别法转向仪器分析法,或是感官鉴别与仪器分析相结合。本文对聚合酶链式反应法(polymerase chain reaction, PCR)、环介导等温扩增技术(loop-mediated isothermal amplification, LAMP)、重组酶聚合酶扩增技术(recombinase polymerase amplification,RPA)、质谱检测法、近红外光谱法、电子鼻与电子舌法等检测方法的研究进展进行了综述,并对其优缺点进行了比较分析,以期为假羊肉的鉴别方法的选择提供借鉴。
In recent years,it is not uncommon to make fake lamb meat from duck,pork,clam meat and rat meat.,the proliferation of fake lamb not only interferes with the market order,but also seriously damages the interests of farmers and consumers.Therefore,it is of great significance to effectively use the mutton authentication technology to combat unscrupulous merchants engaged in the production and operation of fake mutton in order to maintain market order and protect consumer interests.With the development of instrumental analysis techniques,the identification method for mutton meat has changed from the traditional sensory identification method to instrumental analysis,or the combination of sensory identification and instrumental analysis.In this paper,the research progress of detection methods such aspolymerase chain reaction(PCR),loop-mediated isothermal amplification(LAMP),recombinase polymerase amplification(RPA),mass spectrometry,near-infrared spectroscopy,electronic nose and electronic tongue method were reviewed,and their advantages and disadvantageswere compared and analyzed,in order to provide references for the selection of the identification method of fake lamb.
In recent years,it is not uncommon to make fake lamb meat from duck,pork,clam meat and rat meat.,the proliferation of fake lamb not only interferes with the market order,but also seriously damages the interests of farmers and consumers.Therefore,it is of great significance to effectively use the mutton authentication technology to combat unscrupulous merchants engaged in the production and operation of fake mutton in order to maintain market order and protect consumer interests.With the development of instrumental analysis techniques,the identification method for mutton meat has changed from the traditional sensory identification method to instrumental analysis,or the combination of sensory identification and instrumental analysis.In this paper,the research progress of detection methods such aspolymerase chain reaction(PCR),loop-mediated isothermal amplification(LAMP),recombinase polymerase amplification(RPA),mass spectrometry,near-infrared spectroscopy,electronic nose and electronic tongue method were reviewed,and their advantages and disadvantageswere compared and analyzed,in order to provide references for the selection of the identification method of fake lamb.
引文
[1]汤一亮.公安部通报:犯罪团伙用狐狸、老鼠肉冒充羊肉销往市场[DB/OL].[2013-5-4].http://m.cnwest.com/data/html/content/9132590.html Tang YL.Ministry of public security informed:Criminal gangs with fox,rat meat posing as mutton sold in the market China[DB/OL].[2013-5-4].http://m.cnwest.com/data/html/content/9132590.html
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[2]张聪.山东临邑警方披露“假羊肉案”细节:用鸭脯肉造假148吨[DB/OL].[2013-12-4].http://news.iqilu.com/shandong/yuanchuang/2013/1204/1769722.shtml Zhang C.Shandong Linyi police disclosed fake mutton case details:Faked with 148 tons of duck breast meat[DB/OL].[2013-12-4].http://news.iqilu.com/shandong/yuanchuang/2013/1204/1769722.shtml.
[3]王晓易.辽宁曝光假羊肉等十大食品犯罪案例[J].中国防伪报道,2013,(6):102-104.Wang XY.Liaoning exposed fake mutton and other top ten food crime cases[J].China Anti-cou Rep,2013,(6):102-104.
[4]阎婧琦.大连甘区市场监管局查获两处制造假羊肉黑窝点[N].地铁时报,2017-4-14.Yan JQ.Dalian Ganqu market authority seized two manufacturing black sheep dens point[N].Metro Times,2017-4-14.
[5]李欣南,关一夫.掺假肉检验技术发展现状[J].食品研究与开发,2016,37(5):189-193.Li XN,Guan YF.Present situation of testing technology of meat adulteration[J].Food Res Dev,2016,37(5):189-193.
[6]朱雨薇.肉类掺假检测鉴定技术的研究进展[J].食品工业,2014,35(11):242-247.Zhu YW.The development trend of the meat adulteration detection and authentication technology[J].Food Ind,2014,35(11):242-247.
[7]巩红霞,任永宏,巩强.用多重PCR方法鉴定生羊肉的真假[J].中国畜牧兽医,2006,33(8):38-39.Gong HX,Ren YH,Gong Q.Identification of true and false raw lamb by multiplex PCR[J].China Anim Husb Vet Med,2006,33(8):38-39.
[8]Sanders R,Huggett J,Bushell CA,et al.Evaluation ofdigital PCR for absolute DNA quantification[J].Anal Chem,2011,(83):6474-6484.
[9]胡伟,陈荣华,张晨,等.微滴式数字PCR技术用于生物样品种属鉴定和绝对定量[J].法医学杂志,2014,30(5):342-345.Hu W,Chen RH,Zhang C,et al.Species identification and absolute quantification of biological samples by dropletdigital PCR[J].J For Med,2014,30(5):342-345.
[10]董莲华,张玲,姜君,等.大肠杆菌O157:H7微滴数字PCR定量方法的建立[J].分析化学,2015,43(3):319-324.Dong LH,Zhang L,Jiang J,et al.Development of droplet digital polymerase chain reaction for quantifying Escherichia Coli O157:H7[J].Chin J Anal Chem,2015,43(3):319-324.
[11]Morisset D,Stebih D,Milavec M,et al.Quantitative analysisof food and feed samples with droplet digital PCR[J].PLoS One,2013,8(5):1-9.
[12]姜羽,胡佳莹,杨立桃.利用微滴数字PCR分析转基因生物外源基因拷贝数[J].农业生物技术学报,2014,22(10):1298-1305.Jiang Y,Hu JY,Yang LT.Estimating the exogenous genes copy number of genetically modified organisms by droplet digital PCR[J].Chin J Agric Biotechnol,2014,22(10):1298-1305.
[13]Cai YC,Li X,Lv R,et al.Quantitative analysis of porkand chicken products by droplet digital PCR[J].Bioed Res Int,2014,(8):1-6.
[14]朱鹏宇.利用微滴数字PCR定量检测食品或饲料样品[J].农业生物技术学报,2013,(12):1-6.Zhu PY.Quantitative analysis of food and feed samples with droplet digital PCR[J].J Agric Biotechnol,2013,(12):1-6.
[15]Brunetto GS,Massoud R,Leibovitch EC,et al.Digital droplet PCR(ddPCR)for the precise quantification of humantlymphotropic virus 1proviral loads in peripheral blood and cerebrospinal fluid of HAM/TSPpatients and identification of viral mutations[J].J Neurovirol,2014,20(4):341-351
[16]张驰,邱皓璞,张筠.荧光定量PCR检测肉制品中鸭源性成分[J].食品科学,2013,39(18):154-157.Zhang C,Qiu HP,Zhang Y.Quantitative fluorescent PCR method fordetection of duck-derived ingredients in meat products[J].Food Sci,2013,39(18):154-157.
[17]高敬,魏迪,赵癸荣.常见肉类鉴别技术研究进展[J].食品科学,2014,35(11):356-360.Gao J,Wei D,Zhao KR.Recent progress on commonly used techniques for identification of meat species[J].Food Sci,2014,35(11):356-360.
[18]何玮玲,黄明,张驰.食品中肉类成分种属鉴别技术研究进展[J].食品科学,2012,33(3):304-307.He WL,Huang M,Zhang C.Recent technological advances for identification of meat species in food products[J].Food Sci,2012,33(3):304-307.
[19]Dooley JJ,Paine KE,Garrett SD,et al.Detection of meat species using TaqMan real-time PCR assays[J].Meat Sci,2004,68(3):431-438.
[20]Notomi T,Okayama H,Masubuchi H,et al.Loop-mediated isothermalamplification of DNA[J].Nucleic Acid Res,2000,28(12):63.
[21]Bühlmann A,Pothier JF,Rezzonico F,et al.Loop-mediated isotherma lamplification(LAMP)assay for rapid pathogen detection and on-site diagnosis of fire blight[J].J Microbiol Meth,2013,92(3):332-339.
[22]江再茂,马雪萍,殷竹君,等.单管可视化环介导等温扩增技术快速检测恶性疟原虫[J].现代生物医学进展,2014,14(26):5014-5018.Jiang ZM,Ma XP,Yin ZJ,et al.A closed-tube isothermal amplificationmethod for highly sensitive and visualized detection of plasmodiumfalciparum[J].Prog Mod Biomed,2014,14(26):5014-5018.
[23]Scheel CM,Zhou Y,Theodoro RC,et al.Development of a loop-mediatedisothermal amplification method for detection of Histoplasma capsulatum DNA in clinical samples[J].J Clin Microbiol,2014,52(2):483-488.
[24]初亚男,封利颖,张婕妤,等.环介导等温扩增技术改进的研究进展[J].微生物学通报,2015,42(4):729-735.Chu YN,Feng LY,Zhang JY,et al.A systematic review of the researchprogress and improvement of loop-mediated isothermal amplification[J].Microbiol China,2015,42(4):729-735.
[25]李向丽,刘垚,谭贵良,等.基于LAMP法快速检测羊肉及其制品中的猪、鸭和羊源性成分[J].中国食品卫生杂志,2015,27(3):247-252.Li XL,Liu Y,Tan GL,et al.Identification of porcin,duck and sheep-derived materials in mutton and its products by LAMP assay[J].Chin J Food Hyg,2015,27(3):247-252.
[26]杨丽霞,邱华丽,蒋成,等.可视化环介导等温扩增技术检测鸡鸭源性成分[J].食品安全质量检测学报,2017,8(2):624`-628.Yang LX,Qiu H,Jiang C,et al.Visual detection of chicken and duck derived components by loop-mediated isothermal amplification method[J].J Food Saf Qual,2017,8(2):624-628.
[27]曾军辉.可视化环介导等温扩增技术研究进展[J].广东畜牧兽医科技,2016,41(1):8-10,19.Zeng JH.Review on visualizable loop-mediated isothermal amplification[J].Guangdong J Anim Vet Sci,2016,41(1):8-10,19.
[28]罗宝正,赵福振,邵建宏,等.羊源性成分的LAMP检测方法的引物、检测方法和试剂盒.中国:CN105838807A[P].2016-8-10.Luo BZ,Zhao FZ,Shao JH,et al.Primers,detection methods and kits for LAMP detection methods of sheep-derived components.China:CN105838807A[P].2016-8-10.
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