DNA条形码在厦门湾鱼卵和仔稚鱼分类鉴定中的应用
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摘要
本研究选取2017年4月采集的厦门湾鱼卵、仔稚鱼样品进行DNA条形码分析。获得鱼卵、仔稚鱼的COI基因序列27条,共鉴定种类11种,全部鉴定到种,隶属于4目9科11属。在系统进化树的分析中,同一种类的不同个体都聚在一支,所有物种都分别聚为独立的一支,这些物种都能有效地区分开来,说明利用COI基因可以进行有效的仔稚鱼物种鉴定。同时获取11种鱼类浮游生物高清图像,并描述其甄别性的形态特征。以上结果表明,线粒体COI基因作为DNA条形码可以实现鱼卵和仔稚鱼的物种鉴定,且较多能鉴定到种的水平。
In most studies,the ichthyoplankton have been identified by morphological characteristics. Previous studies have shown that DNA barcoding can be used to identify the early life stages of fish more accurately than traditional morphological methods. To study the applicability of this technique to identify ichthyoplankton,samples were collected in Xiamen Bay in April 2017. A total of 27 valid cytochrome C oxidase subunit I( COI) gene sequences of ichthyoplankton were obtained. Eleven species were identified to the level of species. All sequences formed species units in a neighbor-joining phylogenetic tree [based on Kimura-2-parameter( K2 P) distances],indicating that the DNA barcode could be used to identify these 11 species.Consequently,COI barcoding was one of the useful tools to identify ichthyoplankton. The results demonstrated that DNA barcoding was a powerful complement to traditional morphology,and an accurate,standardized,and efficient tool for ichthyoplankton species identification,and possessed a broad prospect of application in fish biodiversity research.
In most studies,the ichthyoplankton have been identified by morphological characteristics. Previous studies have shown that DNA barcoding can be used to identify the early life stages of fish more accurately than traditional morphological methods. To study the applicability of this technique to identify ichthyoplankton,samples were collected in Xiamen Bay in April 2017. A total of 27 valid cytochrome C oxidase subunit I( COI) gene sequences of ichthyoplankton were obtained. Eleven species were identified to the level of species. All sequences formed species units in a neighbor-joining phylogenetic tree [based on Kimura-2-parameter( K2 P) distances],indicating that the DNA barcode could be used to identify these 11 species.Consequently,COI barcoding was one of the useful tools to identify ichthyoplankton. The results demonstrated that DNA barcoding was a powerful complement to traditional morphology,and an accurate,standardized,and efficient tool for ichthyoplankton species identification,and possessed a broad prospect of application in fish biodiversity research.
引文
[1]卞晓东,张秀梅,高天翔,等.沙氏下(鱵)鱼卵的形态学及遗传学鉴别[J].水产学报,2008,32(3):342-352.
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[7] Ward R D,Zemlak T S,Innes B H,et al. DNA barcoding Australia's fish species[J]. Philosophical Transactions Biological Sciences,2005,360(1462):1847-1857.
[8]卞晓东,张秀梅,肖永双,等.线粒体DNA序列在沙氏下鱵鱼鱼卵鉴别上的应用[J].中国海洋大学学报(自然科学版),2007,37(sup. 1):111-116.
[9] Ko H L,Wang Y T,Chiu T S,et al. Evaluating the accuracy of morphological identification of larval fishes by applying DNA barcoding[J]. Plos One,2013,8(1):e53451.
[10]曾祥波,黄邦钦.厦门西海域微型浮游动物的丰度、生物量及其生产力的季节变动[J].厦门大学学报(自然科学版),2010,49(1):109-115.
[11]叶孙忠,马超,刘勇,等.九龙江口秋季游泳生物种类组成及分布特征[J].渔业研究,2017,39(1):54-62.
[12]江素菲,陈枫.九龙江口鱼类浮游生物的生态[J].台湾海峡,1993,(4):351-358.
[13]林楠,沈长春,钟俊生.九龙江口仔、稚鱼种类组成和季节变化[J].南方水产,2009,5(4):1-8.
[14]张跃平.福建省主要海湾浮性鱼卵和仔稚鱼的种类组成与丰度分布[J].台湾海峡,2011,30(2):234-242.
[15]徐春燕,沈长春,刘勇,等.厦门南部海域春季鱼卵、仔稚鱼种类组成与数量分布[J].福建水产,2013,35(6):448-454.
[16]蔡秉及,王志远.厦门港及邻近海域的浮性鱼卵和仔、稚鱼[J].台湾海峡,1994,(2):204-208.
[17]林双淡.厦门港湾底栖鱼类群落结构的基本特征及其季节差异[J].台湾海峡,1985,(2):93-100.
[18]黄良敏,张雅芝,姚舒栓.厦门东海域定置网渔获鱼类种类组成及其季节变化[J].台湾海峡,2006,25(4):509-520.
[19]邢炳鹏,林汝榕,王彦国,等.基于COⅠ基因的厦门海域鱼类DNA条形码鉴定[J].应用海洋学学报,2016,35(1):144-150.
[2] Hebert P D N,Ratnasingham S,Dewaard J R D.Barcoding animal life:cytochrome c oxidase subunit 1divergences among closely related species[J]. Proceedings of the Royal Society B:Biological Sciences,2003,270(Suppl_1):S96-S99.
[3]水戸敏,Mito S.日本近海に出現する浮游性魚卵(II):アカマンボウ目,マトウダイ目,ボラ亜目,サバ亜目,アジ亜目およびイボダイ亜目[J].九州大學農學部學藝雜誌,1961,18:451-466.
[4]张仁斋.中国近海鱼卵与仔鱼[M].上海:上海科学技术出版社,1985.
[5]邵广昭.臺灣魚類資料庫網路電子版[DB/OL].[2008-08-30]. http://fishdb. sinica. edu. tw.
[6]万瑞景.中国近海及其邻近海域鱼卵与仔稚鱼[M].上海:上海科学技术出版社,2016.
[7] Ward R D,Zemlak T S,Innes B H,et al. DNA barcoding Australia's fish species[J]. Philosophical Transactions Biological Sciences,2005,360(1462):1847-1857.
[8]卞晓东,张秀梅,肖永双,等.线粒体DNA序列在沙氏下鱵鱼鱼卵鉴别上的应用[J].中国海洋大学学报(自然科学版),2007,37(sup. 1):111-116.
[9] Ko H L,Wang Y T,Chiu T S,et al. Evaluating the accuracy of morphological identification of larval fishes by applying DNA barcoding[J]. Plos One,2013,8(1):e53451.
[10]曾祥波,黄邦钦.厦门西海域微型浮游动物的丰度、生物量及其生产力的季节变动[J].厦门大学学报(自然科学版),2010,49(1):109-115.
[11]叶孙忠,马超,刘勇,等.九龙江口秋季游泳生物种类组成及分布特征[J].渔业研究,2017,39(1):54-62.
[12]江素菲,陈枫.九龙江口鱼类浮游生物的生态[J].台湾海峡,1993,(4):351-358.
[13]林楠,沈长春,钟俊生.九龙江口仔、稚鱼种类组成和季节变化[J].南方水产,2009,5(4):1-8.
[14]张跃平.福建省主要海湾浮性鱼卵和仔稚鱼的种类组成与丰度分布[J].台湾海峡,2011,30(2):234-242.
[15]徐春燕,沈长春,刘勇,等.厦门南部海域春季鱼卵、仔稚鱼种类组成与数量分布[J].福建水产,2013,35(6):448-454.
[16]蔡秉及,王志远.厦门港及邻近海域的浮性鱼卵和仔、稚鱼[J].台湾海峡,1994,(2):204-208.
[17]林双淡.厦门港湾底栖鱼类群落结构的基本特征及其季节差异[J].台湾海峡,1985,(2):93-100.
[18]黄良敏,张雅芝,姚舒栓.厦门东海域定置网渔获鱼类种类组成及其季节变化[J].台湾海峡,2006,25(4):509-520.
[19]邢炳鹏,林汝榕,王彦国,等.基于COⅠ基因的厦门海域鱼类DNA条形码鉴定[J].应用海洋学学报,2016,35(1):144-150.