银黄参精油对日光性皮炎模型小鼠皮肤损伤的保护作用
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摘要
目的:观察银黄参精油对中波紫外线(UVB)致小鼠日光性皮炎模型的影响。方法:将60只SPF级BALB/c雌性小鼠随机分组空白组,模型组,敏立消组,银黄参精油大、小剂量组,除空白组外,模型组每天背部涂抹生理盐水0.25 mL·cm~(-2),敏立消组每天涂抹敏立消0.012 5 g·cm~(-2),银黄参精油小剂量组0.125 mL·cm~(-2),大剂量0.25 mL·cm~(-2),1次/d,连续给予相应药物8 d。除空白组外,其他各组于第6天在距308 nm紫外线15 cm处照射1.5 h,照射后1 h及照射后24 h再涂搽相应药物1次,48 h后观察皮肤,模型组背部脱毛处有红肿出现,提示造模成功。结果:取部分局部皮肤匀浆液测定一氧化氮(NO),一氧化氮合酶(NOS),肿瘤坏死因子-α(TNF-α)的含量,剩余部分苏木素-伊红(HE)染色观察局部组织病理形态变化。结果:与空白组相比,模型组局部皮肤表观红肿、破溃,组织上皮和附件缺失,表面附着大量炎性细胞及坏死物,局部组织形态病理有显著变化(P<0.01),NO,NOS,TNF-α含量显著升高(P<0.01);与模型组相比,银黄参精油大、小剂量组和敏立消组均可显著减轻局部皮肤组织表观症状(P<0.01),皮肤组织中NO,NOS,TNF-α含量显著降低(P<0.01),显著减轻局部组织形态病理变化(P<0.01)。结论:银黄参精油大、小剂量对小鼠日光性皮炎有较好的防治作用。
Objective:To study the effect of Yinhuangshen essential oil in mice of solar dermatitis model by ultraviolet(UVB).Method:Sixty SPF BALB/c female mice were randomly divided into blank group,model group,Minlixiao group,and large and small-dose Yinhuangshen essential oil groups.Except for blank group,model group was given 0.25 mL·cm~(-2) normal saline on the back every day.The Minlixiao group was given Min Lixiao every day.Low-dose Yinhuangshen essential oil group was given 0.125 mL·cm~(-2),and large-dose group was given 0.25 mL·cm~(-2),once a day,and the corresponding drugs were given continuously for 8 days.Except for blank group,all of the other groups were irradiated for 1.5 hours at 15 cm away from 308 nm UVB lamp on the 6 th day.One hour and 24 hours after irradiation,mice were given corresponding drug once,and skin was observed 48 hours later.Contents of nitric oxide(NO),nitric oxide synthase(NOS) and tumor necrosis factor-alpha(TNF-α)in some local skin homogenates were measured.The histopathological changes were observed by htoxylin eosin(HE) staining.Result:Compared with blank group,skin of model group was reddish,swollen and ulcerated,epithelium and adnexal were absent,a large number of inflammatory cells and necrosis were adhered to the surface,the local histopathological changes significantly(P<0.01),and NO,NOS,TNF-α were significantly increased(P<0.01).Compared with model group,contents of NO,NOS and TNF-α in the skin tissue of Minlixiao group and Yinhuangshen essential oil group were significantly decreased,and it could remarkable reduce the pathological changes in local tissue morphology(P<0.01).Conclusion:Yinhuangshen essential oil has a good effect in preventing and treating solar dermatitis.
Objective:To study the effect of Yinhuangshen essential oil in mice of solar dermatitis model by ultraviolet(UVB).Method:Sixty SPF BALB/c female mice were randomly divided into blank group,model group,Minlixiao group,and large and small-dose Yinhuangshen essential oil groups.Except for blank group,model group was given 0.25 mL·cm~(-2) normal saline on the back every day.The Minlixiao group was given Min Lixiao every day.Low-dose Yinhuangshen essential oil group was given 0.125 mL·cm~(-2),and large-dose group was given 0.25 mL·cm~(-2),once a day,and the corresponding drugs were given continuously for 8 days.Except for blank group,all of the other groups were irradiated for 1.5 hours at 15 cm away from 308 nm UVB lamp on the 6 th day.One hour and 24 hours after irradiation,mice were given corresponding drug once,and skin was observed 48 hours later.Contents of nitric oxide(NO),nitric oxide synthase(NOS) and tumor necrosis factor-alpha(TNF-α)in some local skin homogenates were measured.The histopathological changes were observed by htoxylin eosin(HE) staining.Result:Compared with blank group,skin of model group was reddish,swollen and ulcerated,epithelium and adnexal were absent,a large number of inflammatory cells and necrosis were adhered to the surface,the local histopathological changes significantly(P<0.01),and NO,NOS,TNF-α were significantly increased(P<0.01).Compared with model group,contents of NO,NOS and TNF-α in the skin tissue of Minlixiao group and Yinhuangshen essential oil group were significantly decreased,and it could remarkable reduce the pathological changes in local tissue morphology(P<0.01).Conclusion:Yinhuangshen essential oil has a good effect in preventing and treating solar dermatitis.
引文
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[11]陈向齐,曾抗,刘向农,等.中波紫外线诱导小鼠表皮日晒伤细胞形成的实验研究[J].福建医科大学学报,2006,40(6):547-549.
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[14]韩桂莲.穴位自血疗法治疗日光性皮炎一则[J].中国民间疗法,2015,23(9):27.
[15]丁蕾.美能联合卡介菌多糖、依巴斯汀在小儿日光性皮炎治疗中的应用[J].齐齐哈尔医学院学报,2015,36(1):10-11.
[16]王造林,王玉光.氟芬那酸丁酯软膏治疗日光皮炎的疗效及安全性分析[J].中外医疗,2015,34(28):138-139.
[17]朱春玲.红光联合苦参油治疗外阴营养不良110例临床护理[J].齐鲁护理杂志,2012,18(13):92-93.
[18]丁礼琴,金家骅.GC测定清凉油中薄荷脑、樟脑、桉油精和丁香酚的含量[J].中国实验方剂学杂志,2014,20(13):85-89.
[19]沈梅芳,李小萌,单琪媛.薄荷化学成分与药理作用研究新进展[J].中华中医药学刊,2012,30(7):1484-1487.
[20]庞渤.大黄油对皮肤细菌感染的治疗观察[J].中国医药指南,2013,11(22):262-263.
[21]白明,刘丹丹,缪君娴,等.不同品种大黄油糊外用抗炎作用及对大鼠皮肤溃疡、痔疮模型的影响[J].中国现代应用药学,2013,30(6):575-581.
[22]咸婧,符江,程锦堂,等.药用大黄地上部分化学成分[J].中国实验方剂学杂志,2017,23(14):45-51.
[23]张燕,刘桂丽,李瑞静,等.木天蓼对VEGF121刺激血管内皮细胞分泌NO的影响[J].中国皮肤性病学杂志,2017,31(8):834-837.
[24]顾锦章,蔡益芹.血清一氧化氮测定在炎症性皮肤病中的意义[J].皮肤病与性病,2013,35(5):249,254.
[25]雒玉辉,李树君.血清IFN-γ、IL-2、TNF-α及Ig E水平变化在老年皮肤瘙痒症发病机制中的作用观察[J].中国医药指南,2018,16(19):40-41.
[26]Jr D O D,Rodrigues E B,Maia M,et al.Cytokines in neovascular age-related macular degeneration:fundamentals of targeted combination therapy[J].Br JOphthalmol,2011,95(12):1631-1637.
[2]潘德海,红梅.浅谈日光性皮炎[J].中老年保健,2015(7):14-15.
[3]李燕,王爱琴,申杰,等.重度植物日光性皮炎一例[J].中国麻风皮肤病杂志,2018,34(6):369,377.
[4]李博文,任茜,曹长青,等.2岁儿童食用灰菜致植物日光性皮炎1例[J].临床合理用药杂志,2018,11(7):21,24.
[5]刘志宏,叶小红,张晶,等.复方芦荟乳膏对豚鼠日晒伤的研究[J].中华中医药杂志,2016,31(12):5294-5296.
[6]王艳,马彦,冯文莉,等.鲜蘑菇致植物日光性皮炎一例[J].中华全科医师杂志,2017,16(12):965-966.
[7]王陈君.高原地区日光性皮炎的临床分析[J].健康之路,2018,17(3):54.
[8]陆凌.中西医结合治疗训练中日光性皮炎患者的临床观察[J].中国民康医学,2018,30(12):77-78,82.
[9]方一妙,马丽俐,林金,等.清热凉血中药口服联合鱼腥草滴眼液湿敷治疗面部日光性皮炎临床观察[J].新中医,2018,50(4):154-157.
[10]王景文.京万红软膏治疗皮肤损伤的临床应用研究进展[J].药物评价研究,2014,37(4):375-380.
[11]陈向齐,曾抗,刘向农,等.中波紫外线诱导小鼠表皮日晒伤细胞形成的实验研究[J].福建医科大学学报,2006,40(6):547-549.
[12]李晓鹏,夏丽真,孙强.外用京万红软膏与绿药膏治疗日晒伤疗效观察[J].北京医学,2015,37(11):1092-1093.
[13]Ozkol H U,Akdeniz N,Ozkol H,et al.Development of phytophoto-dermatitis in two cases related to plantago lanceolata[J].J Toxicol Cutan Ocul Toxicol,2012,31(1):58-60.
[14]韩桂莲.穴位自血疗法治疗日光性皮炎一则[J].中国民间疗法,2015,23(9):27.
[15]丁蕾.美能联合卡介菌多糖、依巴斯汀在小儿日光性皮炎治疗中的应用[J].齐齐哈尔医学院学报,2015,36(1):10-11.
[16]王造林,王玉光.氟芬那酸丁酯软膏治疗日光皮炎的疗效及安全性分析[J].中外医疗,2015,34(28):138-139.
[17]朱春玲.红光联合苦参油治疗外阴营养不良110例临床护理[J].齐鲁护理杂志,2012,18(13):92-93.
[18]丁礼琴,金家骅.GC测定清凉油中薄荷脑、樟脑、桉油精和丁香酚的含量[J].中国实验方剂学杂志,2014,20(13):85-89.
[19]沈梅芳,李小萌,单琪媛.薄荷化学成分与药理作用研究新进展[J].中华中医药学刊,2012,30(7):1484-1487.
[20]庞渤.大黄油对皮肤细菌感染的治疗观察[J].中国医药指南,2013,11(22):262-263.
[21]白明,刘丹丹,缪君娴,等.不同品种大黄油糊外用抗炎作用及对大鼠皮肤溃疡、痔疮模型的影响[J].中国现代应用药学,2013,30(6):575-581.
[22]咸婧,符江,程锦堂,等.药用大黄地上部分化学成分[J].中国实验方剂学杂志,2017,23(14):45-51.
[23]张燕,刘桂丽,李瑞静,等.木天蓼对VEGF121刺激血管内皮细胞分泌NO的影响[J].中国皮肤性病学杂志,2017,31(8):834-837.
[24]顾锦章,蔡益芹.血清一氧化氮测定在炎症性皮肤病中的意义[J].皮肤病与性病,2013,35(5):249,254.
[25]雒玉辉,李树君.血清IFN-γ、IL-2、TNF-α及Ig E水平变化在老年皮肤瘙痒症发病机制中的作用观察[J].中国医药指南,2018,16(19):40-41.
[26]Jr D O D,Rodrigues E B,Maia M,et al.Cytokines in neovascular age-related macular degeneration:fundamentals of targeted combination therapy[J].Br JOphthalmol,2011,95(12):1631-1637.