摘要
为了解鱼类耐低氧能力与血红蛋白的相关性,测定了大口鲇(Silurus meridionalis)、乌鳢(Channa argus)、加洲鲈(Micropterus salmoides)、斑点叉尾(Ictalurus punetaus)、黄颡鱼(Pelteobagrus fulvidraco)、泥鳅(Misgurnus anguillicaudatus)、黄鳝(Monopterus albus)、草鱼(Ctenopharyngodon idellus)、翘嘴红鲌(Erythroculter ilishaeformis)、鲢(Hypophthalmichthys molitrix)、鳙(Aristichthys nobilis)和鳜(Siniperca chuatsi)12种鱼的窒息点,同时分别对其血红蛋白进行电泳分析。12种鱼的血红蛋白电泳图谱各不相同,大口鲇、乌鳢、加洲鲈、斑点叉尾、黄颡鱼、泥鳅、黄鳝、草鱼、翘嘴红鲌、鲢、鳙、鳜的血红蛋白分子量加权平均值分别为92.93、93.91、94.61、97.78、98.66、98.95、101.34、104.10、104.73、108.06、108.23、111.84 ku,窒息点分别为0.15、0.18、0.30、0.27、0.36、0.26、0.24、0.39、0.42、0.51、0.46、0.59 mg/L。分析发现,这12种鱼类的窒息点与血红蛋白分子量呈极显著正相关(r=0.925,P<0.01)。
To understand the correlation between the hypoxia tolerance ability and the hemoglobin(Hb) in fish. A total of 12 different species of fish, Catfish(Silurus meridionalis), Snakehead(Channa argus), California Perch(Micropterus salmoides), Channel Catfish(Ictalurus punetaus), Yellow Catfish(Pelteobagrus fulvidraco), Loach(Misgurnus anguillicaudatus), Eel(Monopterus albus), Grass Carp(Ctenopharyngodon idellus), Erythroculter Ilishaeformis(Erythroculter ilishaeformis), Silver Carp(Hypophthalmichthys molitrix), Big Head Carp(Aristichthys nobilis) and Chinese Perch(Siniperca chuatsi),were collected from Luhu Fishery and suburban of Wuhan. The hemoglobin was extracted from blood of each species separately. By using vertical polyacrylamide gel electrophoresis technology, the hemoglobin of each species was analyzed. Compared to logarithm of the standard protein molecular weight and mobility of standard curve, the mobility and standard curve of hemoglobin molecular weight was obtained after electrophoresis. With gel image analysis software Bandscan V5.0, the relative content of each hemoglobin band was measured, and the average hemoglobin molecular weight was calculated for each fish. The suffocation points of 12 species fish were determined by closed hydrostatic device. The results showed that the hemoglobin electrophoretograms were not identical(Fig. 1). Their hemoglobin molecular weights were: 92.93, 93.91, 94.61, 97.78, 98.66, 98.95, 101.34, 104.10, 104.73, 108.06, 108.23, 111.84(ku), respectively, and their asphyxiation points were 0.15, 0.18, 0.30, 0.27, 0.36, 0.26, 0.24, 0.39, 0.42, 0.51, 0.46 and 0.59(mg/L), respectively(Table 3). The correlation analysis found that asphyxiation point and the hemoglobin molecular weight were significantly positive correlated(r = 0.925, P < 0.01).
引文
Abdu I A.2004.Oxygen therapeuties:can we tame hemoglobin?Nature Reviews:Drug Discovery,3(2):152-159.
Herrmann J P,Enders E C.2000.Effect of body size on the standard metabolism of horse mackerel.Journal of Fish Biology,57(3):746-760.
Mwangangi D M,Mutungi G.1994.The effects of temperature acclimation on the oxygen consumption and enzyme activity of red and white muscle fibres isolated from the tropical freshwater fish Oreochromis niloticus.Journal of Fish Biology,44(6):1033-1043.
Terwilliger N B.1998.Function adaptations of oxygen-transport proteins.Journal of Experimental Biology,201(8):1085-1098.
蔡磊,白俊杰,李胜杰,等.2012.大口黑鲈幼鱼低温耐受、耗氧率和窒息点研究.上海海洋大学学报,21(6):971-975.
陈宁生,施6芳.1955a.草鱼、白鲢和花鲢的耗氧率.动物学报,7(1):43-57.
陈宁生,施6芳.1955b.饲养鱼窒息现象的研究.水生生物学报,(1):1-6.
陈琴,罗仁生,欧阳贤华,等.2001.斑点叉尾鱼苗阶段耗氧率与窒息点的测定.广西科学院学报,17(1):34-36.
陈松波,陈伟兴,范兆廷.2004.鱼类呼吸代谢研究进展.水产学杂志,17(1):82-88.
杜国玺,张春林,纪慧君.1983.速度滑冰运动员血红蛋白含量赛前、赛后的变化.冰雪运动,5(2):16-26.
樊廷俊,徐彬,荆昭,等.2011.动物血红蛋白结构与功能的研究进展.山东大学学报,46(7):1-6.
姜友陆.1978.物质的量及其单位摩尔的概念和应用.化学通报,(6):34-37.
雷建军,何安尤,施军,等.2005.翘嘴红鲌鱼苗耗氧率和窒息点的观测.广西水产科技,(3):10-12.
罗相忠,邹桂伟,潘光碧.1997.大口鲇耗氧率与窒息点的初步研究.淡水渔业,(3):21-23.
司亚东,陈英鸿,曾继参.1995.鳜鱼的耗氧率及其池塘养殖.水生生物学报,19(4):327-332.
王镜岩,朱胜庚,徐长法.2002.生物化学:上册.3版.北京:高等教育出版社,257-270.
吴鹤龄,林锦湖.1983.遗传学实验方法和技术.北京:高等教育出版社,147-153.
徐兴川,高光明,蒋火金.2004.黄鳝集约化养殖与病害防治新技术.北京:中国农业出版社,35-58.
杨凯,樊启学,张磊,等.2012.黄颡鱼瞬时耗氧率与窒息点的研究.水生态学杂志,33(2):127-131.
叶奕佐.1959.鱼苗、鱼种耗氧率、能需量、窒息点及呼吸系数的初步报告.动物学报,11(2):117-135.
张新铖,朱新平,陈昆慈,等.2012.乌鳢、斑鳢及杂交种耗氧率与窒息点的初步研究.广东农业科学,(9):116-118.
赵振山,印杰,高贵琴,等.1999.泥鳅与大鳞副泥鳅耗氧率与窒息点的研究.水利渔业,19(1):2-3.